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In vitro characterization of human dental pulp stem cells isolated by three different methods
OBJECTIVES: In this study, we characterized human dental pulp cells (HDPCs) obtained by different culture methods to establish the most suitable methodology for dental tissue engineering and regenerative endodontic applications. MATERIALS AND METHODS: HDPCs were isolated by the outgrowth method (HDP...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Academy of Conservative Dentistry
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5107430/ https://www.ncbi.nlm.nih.gov/pubmed/27847750 http://dx.doi.org/10.5395/rde.2016.41.4.283 |
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author | Jang, Ji-Hyun Lee, Hyeon-Woo Cho, Kyu Min Shin, Hee-Woong Kang, Mo Kwan Park, Sang Hyuk Kim, Euiseong |
author_facet | Jang, Ji-Hyun Lee, Hyeon-Woo Cho, Kyu Min Shin, Hee-Woong Kang, Mo Kwan Park, Sang Hyuk Kim, Euiseong |
author_sort | Jang, Ji-Hyun |
collection | PubMed |
description | OBJECTIVES: In this study, we characterized human dental pulp cells (HDPCs) obtained by different culture methods to establish the most suitable methodology for dental tissue engineering and regenerative endodontic applications. MATERIALS AND METHODS: HDPCs were isolated by the outgrowth method (HDPCs-OG), the enzymatic digestion method (collagenase/dispase/trypsin, HDPCs-ED), or the combination of both methods (HDPCs-Combined). The expression of mesenchymal stem cell markers (CD105, CD90, and CD73) was investigated. In vitro differentiation capacities of HDPCs into adipogenic, osteogenic, and chondrogenic lineages were compared. Differentiation markers were analyzed by quantitative reverse-transcription polymerase chain reaction (RT-PCR) and western blotting. RESULTS: Our data indicated that whole HDPCs-ED, HPDCs-OG, and HDPCs-Combined could be differentiated into adipogenic, chrondrogenic, and osteogenic cell types. However, we found that the methods for isolating and culturing HDPCs influence the differentiation capacities of cells. HDPCs-OG and HDPCs-ED were preferably differentiated into adipogenic and osteogenic cells, respectively. Differentiation markers shown by RT-PCR and western blotting analysis were mostly upregulated in the treated groups compared with the control groups. CONCLUSIONS: Our findings confirmed that cell populations formed by two different culture methods and the combined culture method exhibited different properties. The results of this study could provide an insight into regenerative endodontic treatment using HDPCs. |
format | Online Article Text |
id | pubmed-5107430 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | The Korean Academy of Conservative Dentistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-51074302016-11-15 In vitro characterization of human dental pulp stem cells isolated by three different methods Jang, Ji-Hyun Lee, Hyeon-Woo Cho, Kyu Min Shin, Hee-Woong Kang, Mo Kwan Park, Sang Hyuk Kim, Euiseong Restor Dent Endod Research Article OBJECTIVES: In this study, we characterized human dental pulp cells (HDPCs) obtained by different culture methods to establish the most suitable methodology for dental tissue engineering and regenerative endodontic applications. MATERIALS AND METHODS: HDPCs were isolated by the outgrowth method (HDPCs-OG), the enzymatic digestion method (collagenase/dispase/trypsin, HDPCs-ED), or the combination of both methods (HDPCs-Combined). The expression of mesenchymal stem cell markers (CD105, CD90, and CD73) was investigated. In vitro differentiation capacities of HDPCs into adipogenic, osteogenic, and chondrogenic lineages were compared. Differentiation markers were analyzed by quantitative reverse-transcription polymerase chain reaction (RT-PCR) and western blotting. RESULTS: Our data indicated that whole HDPCs-ED, HPDCs-OG, and HDPCs-Combined could be differentiated into adipogenic, chrondrogenic, and osteogenic cell types. However, we found that the methods for isolating and culturing HDPCs influence the differentiation capacities of cells. HDPCs-OG and HDPCs-ED were preferably differentiated into adipogenic and osteogenic cells, respectively. Differentiation markers shown by RT-PCR and western blotting analysis were mostly upregulated in the treated groups compared with the control groups. CONCLUSIONS: Our findings confirmed that cell populations formed by two different culture methods and the combined culture method exhibited different properties. The results of this study could provide an insight into regenerative endodontic treatment using HDPCs. The Korean Academy of Conservative Dentistry 2016-11 2016-10-12 /pmc/articles/PMC5107430/ /pubmed/27847750 http://dx.doi.org/10.5395/rde.2016.41.4.283 Text en ©Copyrights 2016. The Korean Academy of Conservative Dentistry. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Jang, Ji-Hyun Lee, Hyeon-Woo Cho, Kyu Min Shin, Hee-Woong Kang, Mo Kwan Park, Sang Hyuk Kim, Euiseong In vitro characterization of human dental pulp stem cells isolated by three different methods |
title | In vitro characterization of human dental pulp stem cells isolated by three different methods |
title_full | In vitro characterization of human dental pulp stem cells isolated by three different methods |
title_fullStr | In vitro characterization of human dental pulp stem cells isolated by three different methods |
title_full_unstemmed | In vitro characterization of human dental pulp stem cells isolated by three different methods |
title_short | In vitro characterization of human dental pulp stem cells isolated by three different methods |
title_sort | in vitro characterization of human dental pulp stem cells isolated by three different methods |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5107430/ https://www.ncbi.nlm.nih.gov/pubmed/27847750 http://dx.doi.org/10.5395/rde.2016.41.4.283 |
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