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Altered pattern of tumor necrosis factor-alpha production in peripheral blood monocytes from Crohn's disease
AIM: To evaluate the inflammatory state in Crohn’s disease (CD) patients and correlate it with genetic background and microbial spreading. METHODS: By means of flow cytometry, production of tumor necrosis factor-alpha (TNF-α) was measured in peripheral blood monocytes from patients suffering from CD...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Baishideng Publishing Group Inc
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5107593/ https://www.ncbi.nlm.nih.gov/pubmed/27895399 http://dx.doi.org/10.3748/wjg.v22.i41.9117 |
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author | Loganes, Claudia Pin, Alessia Naviglio, Samuele Girardelli, Martina Bianco, Anna Monica Martelossi, Stefano Tommasini, Alberto Piscianz, Elisa |
author_facet | Loganes, Claudia Pin, Alessia Naviglio, Samuele Girardelli, Martina Bianco, Anna Monica Martelossi, Stefano Tommasini, Alberto Piscianz, Elisa |
author_sort | Loganes, Claudia |
collection | PubMed |
description | AIM: To evaluate the inflammatory state in Crohn’s disease (CD) patients and correlate it with genetic background and microbial spreading. METHODS: By means of flow cytometry, production of tumor necrosis factor-alpha (TNF-α) was measured in peripheral blood monocytes from patients suffering from CD, ulcerative colitis (UC) and in healthy subjects after stimulation of the NOD2 and TLR pathways. CD patients were genotyped for the three most common NOD2 variants (R702W, G908R and L1007Pfs*2) and basal production of TNF-α was correlated to NOD2 genotype. Also, production of TNF-α was correlated to plasmatic levels of LPS Binding Protein (LBP), soluble (s) CD14 and to the activity state of the disease. RESULTS: The patients with CD were characterized by a significantly higher monocyte basal expression of TNF-α compared with healthy subjects and UC patients, and after stimulation with Pam(3)CSK(4) (ligand of TLR2/1) and MDP-L18 (ligand of NOD2) this difference was maintained, while other microbial stimuli (LPS, ligand of TLR4 and PolyI:C, ligand of TLR3) induced massive activation in CD monocytes as well as in UC and in healthy control cells. There was no significant difference in the production of TNF-α between patients who carried CD-associated heterozygous or homozygous variants in NOD2 and patients with wild type NOD2 genotype. Although serum LBP levels have been shown to correlate positively with the state of activity of the disease, TNF-α production did not show a clear correlation with either LBP or sCD14 levels in plasma. Moreover, no clear correlation was seen between TNF-α production and activity indices in either CD or UC. CONCLUSION: Peripheral monocytes from CD express higher basal and stimulated TNF-α than controls, regardless of NOD2 genotype and without a clear correlation with disease activity. |
format | Online Article Text |
id | pubmed-5107593 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Baishideng Publishing Group Inc |
record_format | MEDLINE/PubMed |
spelling | pubmed-51075932016-11-28 Altered pattern of tumor necrosis factor-alpha production in peripheral blood monocytes from Crohn's disease Loganes, Claudia Pin, Alessia Naviglio, Samuele Girardelli, Martina Bianco, Anna Monica Martelossi, Stefano Tommasini, Alberto Piscianz, Elisa World J Gastroenterol Basic Study AIM: To evaluate the inflammatory state in Crohn’s disease (CD) patients and correlate it with genetic background and microbial spreading. METHODS: By means of flow cytometry, production of tumor necrosis factor-alpha (TNF-α) was measured in peripheral blood monocytes from patients suffering from CD, ulcerative colitis (UC) and in healthy subjects after stimulation of the NOD2 and TLR pathways. CD patients were genotyped for the three most common NOD2 variants (R702W, G908R and L1007Pfs*2) and basal production of TNF-α was correlated to NOD2 genotype. Also, production of TNF-α was correlated to plasmatic levels of LPS Binding Protein (LBP), soluble (s) CD14 and to the activity state of the disease. RESULTS: The patients with CD were characterized by a significantly higher monocyte basal expression of TNF-α compared with healthy subjects and UC patients, and after stimulation with Pam(3)CSK(4) (ligand of TLR2/1) and MDP-L18 (ligand of NOD2) this difference was maintained, while other microbial stimuli (LPS, ligand of TLR4 and PolyI:C, ligand of TLR3) induced massive activation in CD monocytes as well as in UC and in healthy control cells. There was no significant difference in the production of TNF-α between patients who carried CD-associated heterozygous or homozygous variants in NOD2 and patients with wild type NOD2 genotype. Although serum LBP levels have been shown to correlate positively with the state of activity of the disease, TNF-α production did not show a clear correlation with either LBP or sCD14 levels in plasma. Moreover, no clear correlation was seen between TNF-α production and activity indices in either CD or UC. CONCLUSION: Peripheral monocytes from CD express higher basal and stimulated TNF-α than controls, regardless of NOD2 genotype and without a clear correlation with disease activity. Baishideng Publishing Group Inc 2016-11-07 2016-11-07 /pmc/articles/PMC5107593/ /pubmed/27895399 http://dx.doi.org/10.3748/wjg.v22.i41.9117 Text en ©The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved. http://creativecommons.org/licenses/by-nc/4.0/ This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. |
spellingShingle | Basic Study Loganes, Claudia Pin, Alessia Naviglio, Samuele Girardelli, Martina Bianco, Anna Monica Martelossi, Stefano Tommasini, Alberto Piscianz, Elisa Altered pattern of tumor necrosis factor-alpha production in peripheral blood monocytes from Crohn's disease |
title | Altered pattern of tumor necrosis factor-alpha production in peripheral blood monocytes from Crohn's disease |
title_full | Altered pattern of tumor necrosis factor-alpha production in peripheral blood monocytes from Crohn's disease |
title_fullStr | Altered pattern of tumor necrosis factor-alpha production in peripheral blood monocytes from Crohn's disease |
title_full_unstemmed | Altered pattern of tumor necrosis factor-alpha production in peripheral blood monocytes from Crohn's disease |
title_short | Altered pattern of tumor necrosis factor-alpha production in peripheral blood monocytes from Crohn's disease |
title_sort | altered pattern of tumor necrosis factor-alpha production in peripheral blood monocytes from crohn's disease |
topic | Basic Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5107593/ https://www.ncbi.nlm.nih.gov/pubmed/27895399 http://dx.doi.org/10.3748/wjg.v22.i41.9117 |
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