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Molecular detection and characterization of Brucella species in raw informally marketed milk from Uganda
This study identified and characterized Brucella species in the informal milk chain in Uganda. A total of 324 cattle bulk milk samples were screened for the genus Brucella by real-time PCR with primers targeting the bcsp31 gene and further characterized by the omp25 gene. Of the samples tested, 6.5%...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Co-Action Publishing
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5107632/ https://www.ncbi.nlm.nih.gov/pubmed/27839533 http://dx.doi.org/10.3402/iee.v6.32442 |
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author | Hoffman, Tove Rock, Kim Mugizi, Denis Rwabiita Muradrasoli, Shaman Lindahl-Rajala, Elisabeth Erume, Joseph Magnusson, Ulf Lundkvist, Åke Boqvist, Sofia |
author_facet | Hoffman, Tove Rock, Kim Mugizi, Denis Rwabiita Muradrasoli, Shaman Lindahl-Rajala, Elisabeth Erume, Joseph Magnusson, Ulf Lundkvist, Åke Boqvist, Sofia |
author_sort | Hoffman, Tove |
collection | PubMed |
description | This study identified and characterized Brucella species in the informal milk chain in Uganda. A total of 324 cattle bulk milk samples were screened for the genus Brucella by real-time PCR with primers targeting the bcsp31 gene and further characterized by the omp25 gene. Of the samples tested, 6.5% were positive for Brucella species. In the omp25 phylogeny, the study sequences were found to form a separate clade within the branch containing B. abortus sequences. The study shows that informally marketed cattle milk in Uganda is a likely risk factor for human brucellosis and confirms that B. abortus is present in the cattle population. This information is important for potential future control measures, such as vaccination of cattle. |
format | Online Article Text |
id | pubmed-5107632 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Co-Action Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-51076322016-11-18 Molecular detection and characterization of Brucella species in raw informally marketed milk from Uganda Hoffman, Tove Rock, Kim Mugizi, Denis Rwabiita Muradrasoli, Shaman Lindahl-Rajala, Elisabeth Erume, Joseph Magnusson, Ulf Lundkvist, Åke Boqvist, Sofia Infect Ecol Epidemiol Short Communication This study identified and characterized Brucella species in the informal milk chain in Uganda. A total of 324 cattle bulk milk samples were screened for the genus Brucella by real-time PCR with primers targeting the bcsp31 gene and further characterized by the omp25 gene. Of the samples tested, 6.5% were positive for Brucella species. In the omp25 phylogeny, the study sequences were found to form a separate clade within the branch containing B. abortus sequences. The study shows that informally marketed cattle milk in Uganda is a likely risk factor for human brucellosis and confirms that B. abortus is present in the cattle population. This information is important for potential future control measures, such as vaccination of cattle. Co-Action Publishing 2016-11-11 /pmc/articles/PMC5107632/ /pubmed/27839533 http://dx.doi.org/10.3402/iee.v6.32442 Text en © 2016 Tove Hoffman et al. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Communication Hoffman, Tove Rock, Kim Mugizi, Denis Rwabiita Muradrasoli, Shaman Lindahl-Rajala, Elisabeth Erume, Joseph Magnusson, Ulf Lundkvist, Åke Boqvist, Sofia Molecular detection and characterization of Brucella species in raw informally marketed milk from Uganda |
title | Molecular detection and characterization of Brucella species in raw informally marketed milk from Uganda |
title_full | Molecular detection and characterization of Brucella species in raw informally marketed milk from Uganda |
title_fullStr | Molecular detection and characterization of Brucella species in raw informally marketed milk from Uganda |
title_full_unstemmed | Molecular detection and characterization of Brucella species in raw informally marketed milk from Uganda |
title_short | Molecular detection and characterization of Brucella species in raw informally marketed milk from Uganda |
title_sort | molecular detection and characterization of brucella species in raw informally marketed milk from uganda |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5107632/ https://www.ncbi.nlm.nih.gov/pubmed/27839533 http://dx.doi.org/10.3402/iee.v6.32442 |
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