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Experimental challenge of pregnant cattle with the putative abortifacient Waddlia chondrophila

Waddlia chondrophila is a Gram-negative intracellular bacterial organism that is related to classical chlamydial species and has been implicated as a cause of abortion in cattle. Despite an increasing number of observational studies linking W. chondrophila infection to cattle abortion, little direct...

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Autores principales: Wheelhouse, Nicholas, Flockhart, Allen, Aitchison, Kevin, Livingstone, Morag, Finlayson, Jeanie, Flachon, Virginie, Sellal, Eric, Dagleish, Mark P., Longbottom, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5107887/
https://www.ncbi.nlm.nih.gov/pubmed/27841350
http://dx.doi.org/10.1038/srep37150
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author Wheelhouse, Nicholas
Flockhart, Allen
Aitchison, Kevin
Livingstone, Morag
Finlayson, Jeanie
Flachon, Virginie
Sellal, Eric
Dagleish, Mark P.
Longbottom, David
author_facet Wheelhouse, Nicholas
Flockhart, Allen
Aitchison, Kevin
Livingstone, Morag
Finlayson, Jeanie
Flachon, Virginie
Sellal, Eric
Dagleish, Mark P.
Longbottom, David
author_sort Wheelhouse, Nicholas
collection PubMed
description Waddlia chondrophila is a Gram-negative intracellular bacterial organism that is related to classical chlamydial species and has been implicated as a cause of abortion in cattle. Despite an increasing number of observational studies linking W. chondrophila infection to cattle abortion, little direct experimental evidence exists. Given this paucity of direct evidence the current study was carried out to investigate whether experimental challenge of pregnant cattle with W. chondrophila would result in infection and abortion. Nine pregnant Friesian-Holstein heifers received 2 × 10(8) inclusion forming units (IFU) W. chondrophila intravenously on day 105–110 of pregnancy, while four negative-control animals underwent mock challenge. Only one of the challenged animals showed pathogen-associated lesions, with the organism being detected in the diseased placenta. Importantly, the organism was re-isolated and its identity confirmed by whole genome sequencing, confirming Koch’s third and fourth postulates. However, while infection of the placenta was observed, the experimental challenge in this study did not confirm the abortifacient potential of the organism.
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spelling pubmed-51078872016-11-22 Experimental challenge of pregnant cattle with the putative abortifacient Waddlia chondrophila Wheelhouse, Nicholas Flockhart, Allen Aitchison, Kevin Livingstone, Morag Finlayson, Jeanie Flachon, Virginie Sellal, Eric Dagleish, Mark P. Longbottom, David Sci Rep Article Waddlia chondrophila is a Gram-negative intracellular bacterial organism that is related to classical chlamydial species and has been implicated as a cause of abortion in cattle. Despite an increasing number of observational studies linking W. chondrophila infection to cattle abortion, little direct experimental evidence exists. Given this paucity of direct evidence the current study was carried out to investigate whether experimental challenge of pregnant cattle with W. chondrophila would result in infection and abortion. Nine pregnant Friesian-Holstein heifers received 2 × 10(8) inclusion forming units (IFU) W. chondrophila intravenously on day 105–110 of pregnancy, while four negative-control animals underwent mock challenge. Only one of the challenged animals showed pathogen-associated lesions, with the organism being detected in the diseased placenta. Importantly, the organism was re-isolated and its identity confirmed by whole genome sequencing, confirming Koch’s third and fourth postulates. However, while infection of the placenta was observed, the experimental challenge in this study did not confirm the abortifacient potential of the organism. Nature Publishing Group 2016-11-14 /pmc/articles/PMC5107887/ /pubmed/27841350 http://dx.doi.org/10.1038/srep37150 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Wheelhouse, Nicholas
Flockhart, Allen
Aitchison, Kevin
Livingstone, Morag
Finlayson, Jeanie
Flachon, Virginie
Sellal, Eric
Dagleish, Mark P.
Longbottom, David
Experimental challenge of pregnant cattle with the putative abortifacient Waddlia chondrophila
title Experimental challenge of pregnant cattle with the putative abortifacient Waddlia chondrophila
title_full Experimental challenge of pregnant cattle with the putative abortifacient Waddlia chondrophila
title_fullStr Experimental challenge of pregnant cattle with the putative abortifacient Waddlia chondrophila
title_full_unstemmed Experimental challenge of pregnant cattle with the putative abortifacient Waddlia chondrophila
title_short Experimental challenge of pregnant cattle with the putative abortifacient Waddlia chondrophila
title_sort experimental challenge of pregnant cattle with the putative abortifacient waddlia chondrophila
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5107887/
https://www.ncbi.nlm.nih.gov/pubmed/27841350
http://dx.doi.org/10.1038/srep37150
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