Quantitative fluorescence spectroscopy and flow cytometry analyses of cell-penetrating peptides internalization pathways: optimization, pitfalls, comparison with mass spectrometry quantification

The mechanism of cell-penetrating peptides entry into cells is unclear, preventing the development of more efficient vectors for biotechnological or therapeutic purposes. Here, we developed a protocol relying on fluorometry to distinguish endocytosis from direct membrane translocation, using Penetra...

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Detalles Bibliográficos
Autores principales: Illien, Françoise, Rodriguez, Nicolas, Amoura, Mehdi, Joliot, Alain, Pallerla, Manjula, Cribier, Sophie, Burlina, Fabienne, Sagan, Sandrine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5107916/
https://www.ncbi.nlm.nih.gov/pubmed/27841303
http://dx.doi.org/10.1038/srep36938

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5107916/
https://www.ncbi.nlm.nih.gov/pubmed/27841303
http://dx.doi.org/10.1038/srep36938

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