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Real-Time Detection of a Self-Replicating RNA Enzyme
A system was developed to detect the self-replication of an RNA enzyme in real time. The enzyme is an RNA ligase that undergoes exponential amplification at a constant temperature and can be made to operate in a ligand-dependent manner. The real-time system is based on a fluorimetric readout that di...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5108293/ https://www.ncbi.nlm.nih.gov/pubmed/27706059 http://dx.doi.org/10.3390/molecules21101310 |
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author | Olea, Charles Joyce, Gerald F. |
author_facet | Olea, Charles Joyce, Gerald F. |
author_sort | Olea, Charles |
collection | PubMed |
description | A system was developed to detect the self-replication of an RNA enzyme in real time. The enzyme is an RNA ligase that undergoes exponential amplification at a constant temperature and can be made to operate in a ligand-dependent manner. The real-time system is based on a fluorimetric readout that directly couples the ligation event to an increase in florescence signal that can be monitored using standard instrumentation. The real-time system can also operate entirely with l-RNA, which is not susceptible to degradation by ribonucleases that are present in biological samples. The system is analogous to real-time PCR, but with the potential to detect small molecules, proteins, and other targets that can be recognized by a suitable aptamer. The ligand-dependent self-replication of RNA has potential applications in molecular diagnostics and biosensing that benefit from the rapid, precise, and real-time detection of various target molecules. |
format | Online Article Text |
id | pubmed-5108293 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-51082932017-09-30 Real-Time Detection of a Self-Replicating RNA Enzyme Olea, Charles Joyce, Gerald F. Molecules Article A system was developed to detect the self-replication of an RNA enzyme in real time. The enzyme is an RNA ligase that undergoes exponential amplification at a constant temperature and can be made to operate in a ligand-dependent manner. The real-time system is based on a fluorimetric readout that directly couples the ligation event to an increase in florescence signal that can be monitored using standard instrumentation. The real-time system can also operate entirely with l-RNA, which is not susceptible to degradation by ribonucleases that are present in biological samples. The system is analogous to real-time PCR, but with the potential to detect small molecules, proteins, and other targets that can be recognized by a suitable aptamer. The ligand-dependent self-replication of RNA has potential applications in molecular diagnostics and biosensing that benefit from the rapid, precise, and real-time detection of various target molecules. MDPI 2016-09-30 /pmc/articles/PMC5108293/ /pubmed/27706059 http://dx.doi.org/10.3390/molecules21101310 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Olea, Charles Joyce, Gerald F. Real-Time Detection of a Self-Replicating RNA Enzyme |
title | Real-Time Detection of a Self-Replicating RNA Enzyme |
title_full | Real-Time Detection of a Self-Replicating RNA Enzyme |
title_fullStr | Real-Time Detection of a Self-Replicating RNA Enzyme |
title_full_unstemmed | Real-Time Detection of a Self-Replicating RNA Enzyme |
title_short | Real-Time Detection of a Self-Replicating RNA Enzyme |
title_sort | real-time detection of a self-replicating rna enzyme |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5108293/ https://www.ncbi.nlm.nih.gov/pubmed/27706059 http://dx.doi.org/10.3390/molecules21101310 |
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