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MicroRNA-124 promotes hepatic triglyceride accumulation through targeting tribbles homolog 3

An increase in hepatic triglyceride (TG) contents usually results in non-alcoholic fatty liver disease (NAFLD) and related metabolic diseases. However, the mechanisms underlying perturbations of hepatic TG homeostasis remain largely unknown. Here, we showed that MicroRNA-124 was up-regulated in the...

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Autores principales: Liu, Xing, Zhao, Jiejie, Liu, Qi, Xiong, Xuelian, Zhang, Zhijian, Jiao, Yang, Li, Xiaoying, Liu, Bin, Li, Yao, Lu, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5109039/
https://www.ncbi.nlm.nih.gov/pubmed/27845424
http://dx.doi.org/10.1038/srep37170
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author Liu, Xing
Zhao, Jiejie
Liu, Qi
Xiong, Xuelian
Zhang, Zhijian
Jiao, Yang
Li, Xiaoying
Liu, Bin
Li, Yao
Lu, Yan
author_facet Liu, Xing
Zhao, Jiejie
Liu, Qi
Xiong, Xuelian
Zhang, Zhijian
Jiao, Yang
Li, Xiaoying
Liu, Bin
Li, Yao
Lu, Yan
author_sort Liu, Xing
collection PubMed
description An increase in hepatic triglyceride (TG) contents usually results in non-alcoholic fatty liver disease (NAFLD) and related metabolic diseases. However, the mechanisms underlying perturbations of hepatic TG homeostasis remain largely unknown. Here, we showed that MicroRNA-124 was up-regulated in the livers of C57BL/6 mice fed a short-term high-fat-diet (HFD). Adenoviral overexpression of miR-124 in C57BL/6 mice led to accumulation of excessive triglycerides and up-regulation of lipogenic genes in the liver. We further identified tribbles homolog 3 (TRB3) as a direct target of miR-124. AKT signaling, which is negatively regulated by TRB3, was enhanced by miR-124 overexpression. Moreover, restoration of TRB3 expression markedly abolished the effect of miR-124 on hepatic TG metabolism. Therefore, our findings revealed that miR-124 played a role in mediating high-fat-diet induced TG accumulation in the liver.
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spelling pubmed-51090392016-11-25 MicroRNA-124 promotes hepatic triglyceride accumulation through targeting tribbles homolog 3 Liu, Xing Zhao, Jiejie Liu, Qi Xiong, Xuelian Zhang, Zhijian Jiao, Yang Li, Xiaoying Liu, Bin Li, Yao Lu, Yan Sci Rep Article An increase in hepatic triglyceride (TG) contents usually results in non-alcoholic fatty liver disease (NAFLD) and related metabolic diseases. However, the mechanisms underlying perturbations of hepatic TG homeostasis remain largely unknown. Here, we showed that MicroRNA-124 was up-regulated in the livers of C57BL/6 mice fed a short-term high-fat-diet (HFD). Adenoviral overexpression of miR-124 in C57BL/6 mice led to accumulation of excessive triglycerides and up-regulation of lipogenic genes in the liver. We further identified tribbles homolog 3 (TRB3) as a direct target of miR-124. AKT signaling, which is negatively regulated by TRB3, was enhanced by miR-124 overexpression. Moreover, restoration of TRB3 expression markedly abolished the effect of miR-124 on hepatic TG metabolism. Therefore, our findings revealed that miR-124 played a role in mediating high-fat-diet induced TG accumulation in the liver. Nature Publishing Group 2016-11-15 /pmc/articles/PMC5109039/ /pubmed/27845424 http://dx.doi.org/10.1038/srep37170 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Liu, Xing
Zhao, Jiejie
Liu, Qi
Xiong, Xuelian
Zhang, Zhijian
Jiao, Yang
Li, Xiaoying
Liu, Bin
Li, Yao
Lu, Yan
MicroRNA-124 promotes hepatic triglyceride accumulation through targeting tribbles homolog 3
title MicroRNA-124 promotes hepatic triglyceride accumulation through targeting tribbles homolog 3
title_full MicroRNA-124 promotes hepatic triglyceride accumulation through targeting tribbles homolog 3
title_fullStr MicroRNA-124 promotes hepatic triglyceride accumulation through targeting tribbles homolog 3
title_full_unstemmed MicroRNA-124 promotes hepatic triglyceride accumulation through targeting tribbles homolog 3
title_short MicroRNA-124 promotes hepatic triglyceride accumulation through targeting tribbles homolog 3
title_sort microrna-124 promotes hepatic triglyceride accumulation through targeting tribbles homolog 3
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5109039/
https://www.ncbi.nlm.nih.gov/pubmed/27845424
http://dx.doi.org/10.1038/srep37170
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