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Biomechanics of subcellular structures by non-invasive Brillouin microscopy
Cellular biomechanics play a pivotal role in the pathophysiology of several diseases. Unfortunately, current methods to measure biomechanical properties are invasive and mostly limited to the surface of a cell. As a result, the mechanical behaviour of subcellular structures and organelles remains po...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5109042/ https://www.ncbi.nlm.nih.gov/pubmed/27845411 http://dx.doi.org/10.1038/srep37217 |
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author | Antonacci, Giuseppe Braakman, Sietse |
author_facet | Antonacci, Giuseppe Braakman, Sietse |
author_sort | Antonacci, Giuseppe |
collection | PubMed |
description | Cellular biomechanics play a pivotal role in the pathophysiology of several diseases. Unfortunately, current methods to measure biomechanical properties are invasive and mostly limited to the surface of a cell. As a result, the mechanical behaviour of subcellular structures and organelles remains poorly characterised. Here, we show three-dimensional biomechanical images of single cells obtained with non-invasive, non-destructive Brillouin microscopy with an unprecedented spatial resolution. Our results quantify the longitudinal elastic modulus of subcellular structures. In particular, we found the nucleoli to be stiffer than both the nuclear envelope (p < 0.0001) and the surrounding cytoplasm (p < 0.0001). Moreover, we demonstrate the mechanical response of cells to Latrunculin-A, a drug that reduces cell stiffness by preventing cytoskeletal assembly. Our technique can therefore generate valuable insights into cellular biomechanics and its role in pathophysiology. |
format | Online Article Text |
id | pubmed-5109042 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-51090422016-11-25 Biomechanics of subcellular structures by non-invasive Brillouin microscopy Antonacci, Giuseppe Braakman, Sietse Sci Rep Article Cellular biomechanics play a pivotal role in the pathophysiology of several diseases. Unfortunately, current methods to measure biomechanical properties are invasive and mostly limited to the surface of a cell. As a result, the mechanical behaviour of subcellular structures and organelles remains poorly characterised. Here, we show three-dimensional biomechanical images of single cells obtained with non-invasive, non-destructive Brillouin microscopy with an unprecedented spatial resolution. Our results quantify the longitudinal elastic modulus of subcellular structures. In particular, we found the nucleoli to be stiffer than both the nuclear envelope (p < 0.0001) and the surrounding cytoplasm (p < 0.0001). Moreover, we demonstrate the mechanical response of cells to Latrunculin-A, a drug that reduces cell stiffness by preventing cytoskeletal assembly. Our technique can therefore generate valuable insights into cellular biomechanics and its role in pathophysiology. Nature Publishing Group 2016-11-15 /pmc/articles/PMC5109042/ /pubmed/27845411 http://dx.doi.org/10.1038/srep37217 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Antonacci, Giuseppe Braakman, Sietse Biomechanics of subcellular structures by non-invasive Brillouin microscopy |
title | Biomechanics of subcellular structures by non-invasive Brillouin microscopy |
title_full | Biomechanics of subcellular structures by non-invasive Brillouin microscopy |
title_fullStr | Biomechanics of subcellular structures by non-invasive Brillouin microscopy |
title_full_unstemmed | Biomechanics of subcellular structures by non-invasive Brillouin microscopy |
title_short | Biomechanics of subcellular structures by non-invasive Brillouin microscopy |
title_sort | biomechanics of subcellular structures by non-invasive brillouin microscopy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5109042/ https://www.ncbi.nlm.nih.gov/pubmed/27845411 http://dx.doi.org/10.1038/srep37217 |
work_keys_str_mv | AT antonaccigiuseppe biomechanicsofsubcellularstructuresbynoninvasivebrillouinmicroscopy AT braakmansietse biomechanicsofsubcellularstructuresbynoninvasivebrillouinmicroscopy |