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Rapid construction of a whole-genome transposon insertion collection for Shewanella oneidensis by Knockout Sudoku
Whole-genome knockout collections are invaluable for connecting gene sequence to function, yet traditionally, their construction has required an extraordinary technical effort. Here we report a method for the construction and purification of a curated whole-genome collection of single-gene transposo...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5109470/ https://www.ncbi.nlm.nih.gov/pubmed/27830751 http://dx.doi.org/10.1038/ncomms13270 |