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A comparative study of the morphometry of sperm head components in cattle, sheep, and pigs with a computer-assisted fluorescence method

The aim of this study was to compare the sperm nuclear and acrosomal morphometry of three species of domestic artiodactyls; cattle (Bos taurus), sheep (Ovis aries), and pigs (Sus scrofa). Semen smears of twenty ejaculates from each species were fixed and labeled with a propidium iodide-Pisum sativum...

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Autores principales: Yániz, Jesús L, Capistrós, Sara, Vicente-Fiel, Sandra, Hidalgo, Carlos O, Santolaria, Pilar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5109873/
https://www.ncbi.nlm.nih.gov/pubmed/27624987
http://dx.doi.org/10.4103/1008-682X.186877
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author Yániz, Jesús L
Capistrós, Sara
Vicente-Fiel, Sandra
Hidalgo, Carlos O
Santolaria, Pilar
author_facet Yániz, Jesús L
Capistrós, Sara
Vicente-Fiel, Sandra
Hidalgo, Carlos O
Santolaria, Pilar
author_sort Yániz, Jesús L
collection PubMed
description The aim of this study was to compare the sperm nuclear and acrosomal morphometry of three species of domestic artiodactyls; cattle (Bos taurus), sheep (Ovis aries), and pigs (Sus scrofa). Semen smears of twenty ejaculates from each species were fixed and labeled with a propidium iodide-Pisum sativum agglutinin (PI/PSA) combination. Digital images of the sperm nucleus, acrosome, and whole sperm head were captured and analyzed. The use of the PI/PSA combination and CASA-Morph fluorescence-based method allowed the capture, morphometric analysis, and differentiation of most sperm nuclei, acrosomes and whole heads, and the assessment of acrosomal integrity with a high precision in the three species studied. For the size of the head and nuclear area, the relationship between the three species may be summarized as bull > ram > boar. However, for the other morphometric parameters (length, width, and perimeter), there were differences in the relationships between species for sperm nuclei and whole sperm heads. Bull sperm acrosomes were clearly smaller than those in the other species studied and covered a smaller proportion of the sperm head. The acrosomal morphology, small in the bull, large and broad in the sheep, and large, long, and with a pronounced equatorial segment curve in the boar, was species-characteristic. It was concluded that there are clear variations in the size and shape of the sperm head components between the three species studied, the acrosome being the structure showing the most variability, allowing a clear distinction of the spermatozoa of each species.
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spelling pubmed-51098732016-11-25 A comparative study of the morphometry of sperm head components in cattle, sheep, and pigs with a computer-assisted fluorescence method Yániz, Jesús L Capistrós, Sara Vicente-Fiel, Sandra Hidalgo, Carlos O Santolaria, Pilar Asian J Androl Invited Original Article The aim of this study was to compare the sperm nuclear and acrosomal morphometry of three species of domestic artiodactyls; cattle (Bos taurus), sheep (Ovis aries), and pigs (Sus scrofa). Semen smears of twenty ejaculates from each species were fixed and labeled with a propidium iodide-Pisum sativum agglutinin (PI/PSA) combination. Digital images of the sperm nucleus, acrosome, and whole sperm head were captured and analyzed. The use of the PI/PSA combination and CASA-Morph fluorescence-based method allowed the capture, morphometric analysis, and differentiation of most sperm nuclei, acrosomes and whole heads, and the assessment of acrosomal integrity with a high precision in the three species studied. For the size of the head and nuclear area, the relationship between the three species may be summarized as bull > ram > boar. However, for the other morphometric parameters (length, width, and perimeter), there were differences in the relationships between species for sperm nuclei and whole sperm heads. Bull sperm acrosomes were clearly smaller than those in the other species studied and covered a smaller proportion of the sperm head. The acrosomal morphology, small in the bull, large and broad in the sheep, and large, long, and with a pronounced equatorial segment curve in the boar, was species-characteristic. It was concluded that there are clear variations in the size and shape of the sperm head components between the three species studied, the acrosome being the structure showing the most variability, allowing a clear distinction of the spermatozoa of each species. Medknow Publications & Media Pvt Ltd 2016 2016-09-13 /pmc/articles/PMC5109873/ /pubmed/27624987 http://dx.doi.org/10.4103/1008-682X.186877 Text en Copyright: © 2016 AJA, SIMM & SJTU http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Invited Original Article
Yániz, Jesús L
Capistrós, Sara
Vicente-Fiel, Sandra
Hidalgo, Carlos O
Santolaria, Pilar
A comparative study of the morphometry of sperm head components in cattle, sheep, and pigs with a computer-assisted fluorescence method
title A comparative study of the morphometry of sperm head components in cattle, sheep, and pigs with a computer-assisted fluorescence method
title_full A comparative study of the morphometry of sperm head components in cattle, sheep, and pigs with a computer-assisted fluorescence method
title_fullStr A comparative study of the morphometry of sperm head components in cattle, sheep, and pigs with a computer-assisted fluorescence method
title_full_unstemmed A comparative study of the morphometry of sperm head components in cattle, sheep, and pigs with a computer-assisted fluorescence method
title_short A comparative study of the morphometry of sperm head components in cattle, sheep, and pigs with a computer-assisted fluorescence method
title_sort comparative study of the morphometry of sperm head components in cattle, sheep, and pigs with a computer-assisted fluorescence method
topic Invited Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5109873/
https://www.ncbi.nlm.nih.gov/pubmed/27624987
http://dx.doi.org/10.4103/1008-682X.186877
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