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Morphometry and subpopulation structure of Holstein bull spermatozoa: variations in ejaculates and cryopreservation straws

Sperm quality is evaluated for the calculation of sperm dosage in artificial reproductive programs. The most common parameter used is motility, but morphology has a higher potential as a predictor of genetic quality. Morphometry calculations from CASA-Morph technology improve morphological evaluatio...

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Autores principales: Valverde, Anthony, Arenán, Héctor, Sancho, María, Contell, Jesús, Yániz, Jesús, Fernández, Alejandro, Soler, Carles
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5109875/
https://www.ncbi.nlm.nih.gov/pubmed/27678464
http://dx.doi.org/10.4103/1008-682X.187579
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author Valverde, Anthony
Arenán, Héctor
Sancho, María
Contell, Jesús
Yániz, Jesús
Fernández, Alejandro
Soler, Carles
author_facet Valverde, Anthony
Arenán, Héctor
Sancho, María
Contell, Jesús
Yániz, Jesús
Fernández, Alejandro
Soler, Carles
author_sort Valverde, Anthony
collection PubMed
description Sperm quality is evaluated for the calculation of sperm dosage in artificial reproductive programs. The most common parameter used is motility, but morphology has a higher potential as a predictor of genetic quality. Morphometry calculations from CASA-Morph technology improve morphological evaluation and allow mathematical approaches to the problem. Semen from 28 Holstein bulls was collected by artificial vagina, and several ejaculates were studied. After general evaluation, samples were diluted, packaged in 0.25 ml straws, and stored in liquid nitrogen. Two straws per sample were thawed, and slides were processed and stained with Diff-Quik. Samples were analyzed by a CASA-Morph system for eight morphometric parameters. In addition to the “classical” statistical approach, based on variance analysis (revealing differences between animals, ejaculates, and straws), principal component (PC) analysis showed that the variables were grouped into PC1, related to size, and PC2 to shape. Subpopulation structure analysis showed four groups, namely, big, small, short, and narrow from their dominant characteristics, representing 31.0%, 27.3%, 24.1%, and 17.7% of the total population, respectively. The distributions varied between animals and ejaculates, but between straws, there were no differences in only four animals. This modern approach of considering an ejaculate sperm population as divided into subpopulations reflecting quantifiable parameters generated by CASA-Morph systems technology opens a new view on sperm function. This is the first study applying this approach to evaluate different ejaculates and straws from the same individual. More work must be done to improve seminal dose calculations in assisted reproductive programs.
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spelling pubmed-51098752016-11-25 Morphometry and subpopulation structure of Holstein bull spermatozoa: variations in ejaculates and cryopreservation straws Valverde, Anthony Arenán, Héctor Sancho, María Contell, Jesús Yániz, Jesús Fernández, Alejandro Soler, Carles Asian J Androl Invited Original Article Sperm quality is evaluated for the calculation of sperm dosage in artificial reproductive programs. The most common parameter used is motility, but morphology has a higher potential as a predictor of genetic quality. Morphometry calculations from CASA-Morph technology improve morphological evaluation and allow mathematical approaches to the problem. Semen from 28 Holstein bulls was collected by artificial vagina, and several ejaculates were studied. After general evaluation, samples were diluted, packaged in 0.25 ml straws, and stored in liquid nitrogen. Two straws per sample were thawed, and slides were processed and stained with Diff-Quik. Samples were analyzed by a CASA-Morph system for eight morphometric parameters. In addition to the “classical” statistical approach, based on variance analysis (revealing differences between animals, ejaculates, and straws), principal component (PC) analysis showed that the variables were grouped into PC1, related to size, and PC2 to shape. Subpopulation structure analysis showed four groups, namely, big, small, short, and narrow from their dominant characteristics, representing 31.0%, 27.3%, 24.1%, and 17.7% of the total population, respectively. The distributions varied between animals and ejaculates, but between straws, there were no differences in only four animals. This modern approach of considering an ejaculate sperm population as divided into subpopulations reflecting quantifiable parameters generated by CASA-Morph systems technology opens a new view on sperm function. This is the first study applying this approach to evaluate different ejaculates and straws from the same individual. More work must be done to improve seminal dose calculations in assisted reproductive programs. Medknow Publications & Media Pvt Ltd 2016 2016-09-27 /pmc/articles/PMC5109875/ /pubmed/27678464 http://dx.doi.org/10.4103/1008-682X.187579 Text en Copyright: © 2016 AJA, SIMM & SJTU http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Invited Original Article
Valverde, Anthony
Arenán, Héctor
Sancho, María
Contell, Jesús
Yániz, Jesús
Fernández, Alejandro
Soler, Carles
Morphometry and subpopulation structure of Holstein bull spermatozoa: variations in ejaculates and cryopreservation straws
title Morphometry and subpopulation structure of Holstein bull spermatozoa: variations in ejaculates and cryopreservation straws
title_full Morphometry and subpopulation structure of Holstein bull spermatozoa: variations in ejaculates and cryopreservation straws
title_fullStr Morphometry and subpopulation structure of Holstein bull spermatozoa: variations in ejaculates and cryopreservation straws
title_full_unstemmed Morphometry and subpopulation structure of Holstein bull spermatozoa: variations in ejaculates and cryopreservation straws
title_short Morphometry and subpopulation structure of Holstein bull spermatozoa: variations in ejaculates and cryopreservation straws
title_sort morphometry and subpopulation structure of holstein bull spermatozoa: variations in ejaculates and cryopreservation straws
topic Invited Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5109875/
https://www.ncbi.nlm.nih.gov/pubmed/27678464
http://dx.doi.org/10.4103/1008-682X.187579
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