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Identification of Species of Nontuberculous Mycobacteria Clinical Isolates from 8 Provinces of China

Pulmonary diseases caused by nontuberculous mycobacteria (NTM) are increasing in incidence and prevalence worldwide. In this study, we identified NTM species of the clinical isolates from 8 provinces in China, in order to preliminarily provide some basic scientific data in the different species and...

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Autores principales: Liu, Haican, Lian, Lulu, Jiang, Yi, Huang, Mingxiang, Tan, Yunhong, Zhao, Xiuqin, Zhang, Jingrui, Yu, Qin, Liu, Jiao, Dong, Haiyan, Lu, Bing, Wu, Yimou, Wan, Kanglin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5110891/
https://www.ncbi.nlm.nih.gov/pubmed/27882322
http://dx.doi.org/10.1155/2016/2153910
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author Liu, Haican
Lian, Lulu
Jiang, Yi
Huang, Mingxiang
Tan, Yunhong
Zhao, Xiuqin
Zhang, Jingrui
Yu, Qin
Liu, Jiao
Dong, Haiyan
Lu, Bing
Wu, Yimou
Wan, Kanglin
author_facet Liu, Haican
Lian, Lulu
Jiang, Yi
Huang, Mingxiang
Tan, Yunhong
Zhao, Xiuqin
Zhang, Jingrui
Yu, Qin
Liu, Jiao
Dong, Haiyan
Lu, Bing
Wu, Yimou
Wan, Kanglin
author_sort Liu, Haican
collection PubMed
description Pulmonary diseases caused by nontuberculous mycobacteria (NTM) are increasing in incidence and prevalence worldwide. In this study, we identified NTM species of the clinical isolates from 8 provinces in China, in order to preliminarily provide some basic scientific data in the different species and distribution of NTM related to pulmonary disease in China. A total of 523 clinical isolates from patients with tuberculosis (TB) diagnosed clinically from 2005 to 2012 were identified to the species using conventional and molecular methods, including multilocus PCR, rpoB and hsp65 PCR-PRA, hsp65, rpoB, and 16S-23S internal transcribed spacer region sequencing. The isolates were identified into 3 bacterium genera, including NTM, Gordonia bronchialis, and Nocardia farcinica, and, for the 488 NTM isolates, 27 species were identified. For all the 27 species of NTM which were found to cause pulmonary infections in humans, the most prevalent species was M. intracellulare, followed by M. avium and M. abscessus. And seven other species were for the first time identified in patients with TB in China. NTM species identification is very important for distinguishing between tuberculosis and NTM pulmonary diseases, and the species diversity drives the creation of diverse and integrated identification methods with higher accuracy and efficacy.
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spelling pubmed-51108912016-11-23 Identification of Species of Nontuberculous Mycobacteria Clinical Isolates from 8 Provinces of China Liu, Haican Lian, Lulu Jiang, Yi Huang, Mingxiang Tan, Yunhong Zhao, Xiuqin Zhang, Jingrui Yu, Qin Liu, Jiao Dong, Haiyan Lu, Bing Wu, Yimou Wan, Kanglin Biomed Res Int Research Article Pulmonary diseases caused by nontuberculous mycobacteria (NTM) are increasing in incidence and prevalence worldwide. In this study, we identified NTM species of the clinical isolates from 8 provinces in China, in order to preliminarily provide some basic scientific data in the different species and distribution of NTM related to pulmonary disease in China. A total of 523 clinical isolates from patients with tuberculosis (TB) diagnosed clinically from 2005 to 2012 were identified to the species using conventional and molecular methods, including multilocus PCR, rpoB and hsp65 PCR-PRA, hsp65, rpoB, and 16S-23S internal transcribed spacer region sequencing. The isolates were identified into 3 bacterium genera, including NTM, Gordonia bronchialis, and Nocardia farcinica, and, for the 488 NTM isolates, 27 species were identified. For all the 27 species of NTM which were found to cause pulmonary infections in humans, the most prevalent species was M. intracellulare, followed by M. avium and M. abscessus. And seven other species were for the first time identified in patients with TB in China. NTM species identification is very important for distinguishing between tuberculosis and NTM pulmonary diseases, and the species diversity drives the creation of diverse and integrated identification methods with higher accuracy and efficacy. Hindawi Publishing Corporation 2016 2016-11-02 /pmc/articles/PMC5110891/ /pubmed/27882322 http://dx.doi.org/10.1155/2016/2153910 Text en Copyright © 2016 Haican Liu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Liu, Haican
Lian, Lulu
Jiang, Yi
Huang, Mingxiang
Tan, Yunhong
Zhao, Xiuqin
Zhang, Jingrui
Yu, Qin
Liu, Jiao
Dong, Haiyan
Lu, Bing
Wu, Yimou
Wan, Kanglin
Identification of Species of Nontuberculous Mycobacteria Clinical Isolates from 8 Provinces of China
title Identification of Species of Nontuberculous Mycobacteria Clinical Isolates from 8 Provinces of China
title_full Identification of Species of Nontuberculous Mycobacteria Clinical Isolates from 8 Provinces of China
title_fullStr Identification of Species of Nontuberculous Mycobacteria Clinical Isolates from 8 Provinces of China
title_full_unstemmed Identification of Species of Nontuberculous Mycobacteria Clinical Isolates from 8 Provinces of China
title_short Identification of Species of Nontuberculous Mycobacteria Clinical Isolates from 8 Provinces of China
title_sort identification of species of nontuberculous mycobacteria clinical isolates from 8 provinces of china
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5110891/
https://www.ncbi.nlm.nih.gov/pubmed/27882322
http://dx.doi.org/10.1155/2016/2153910
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