Cargando…
Identification of Species of Nontuberculous Mycobacteria Clinical Isolates from 8 Provinces of China
Pulmonary diseases caused by nontuberculous mycobacteria (NTM) are increasing in incidence and prevalence worldwide. In this study, we identified NTM species of the clinical isolates from 8 provinces in China, in order to preliminarily provide some basic scientific data in the different species and...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5110891/ https://www.ncbi.nlm.nih.gov/pubmed/27882322 http://dx.doi.org/10.1155/2016/2153910 |
_version_ | 1782467764630323200 |
---|---|
author | Liu, Haican Lian, Lulu Jiang, Yi Huang, Mingxiang Tan, Yunhong Zhao, Xiuqin Zhang, Jingrui Yu, Qin Liu, Jiao Dong, Haiyan Lu, Bing Wu, Yimou Wan, Kanglin |
author_facet | Liu, Haican Lian, Lulu Jiang, Yi Huang, Mingxiang Tan, Yunhong Zhao, Xiuqin Zhang, Jingrui Yu, Qin Liu, Jiao Dong, Haiyan Lu, Bing Wu, Yimou Wan, Kanglin |
author_sort | Liu, Haican |
collection | PubMed |
description | Pulmonary diseases caused by nontuberculous mycobacteria (NTM) are increasing in incidence and prevalence worldwide. In this study, we identified NTM species of the clinical isolates from 8 provinces in China, in order to preliminarily provide some basic scientific data in the different species and distribution of NTM related to pulmonary disease in China. A total of 523 clinical isolates from patients with tuberculosis (TB) diagnosed clinically from 2005 to 2012 were identified to the species using conventional and molecular methods, including multilocus PCR, rpoB and hsp65 PCR-PRA, hsp65, rpoB, and 16S-23S internal transcribed spacer region sequencing. The isolates were identified into 3 bacterium genera, including NTM, Gordonia bronchialis, and Nocardia farcinica, and, for the 488 NTM isolates, 27 species were identified. For all the 27 species of NTM which were found to cause pulmonary infections in humans, the most prevalent species was M. intracellulare, followed by M. avium and M. abscessus. And seven other species were for the first time identified in patients with TB in China. NTM species identification is very important for distinguishing between tuberculosis and NTM pulmonary diseases, and the species diversity drives the creation of diverse and integrated identification methods with higher accuracy and efficacy. |
format | Online Article Text |
id | pubmed-5110891 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-51108912016-11-23 Identification of Species of Nontuberculous Mycobacteria Clinical Isolates from 8 Provinces of China Liu, Haican Lian, Lulu Jiang, Yi Huang, Mingxiang Tan, Yunhong Zhao, Xiuqin Zhang, Jingrui Yu, Qin Liu, Jiao Dong, Haiyan Lu, Bing Wu, Yimou Wan, Kanglin Biomed Res Int Research Article Pulmonary diseases caused by nontuberculous mycobacteria (NTM) are increasing in incidence and prevalence worldwide. In this study, we identified NTM species of the clinical isolates from 8 provinces in China, in order to preliminarily provide some basic scientific data in the different species and distribution of NTM related to pulmonary disease in China. A total of 523 clinical isolates from patients with tuberculosis (TB) diagnosed clinically from 2005 to 2012 were identified to the species using conventional and molecular methods, including multilocus PCR, rpoB and hsp65 PCR-PRA, hsp65, rpoB, and 16S-23S internal transcribed spacer region sequencing. The isolates were identified into 3 bacterium genera, including NTM, Gordonia bronchialis, and Nocardia farcinica, and, for the 488 NTM isolates, 27 species were identified. For all the 27 species of NTM which were found to cause pulmonary infections in humans, the most prevalent species was M. intracellulare, followed by M. avium and M. abscessus. And seven other species were for the first time identified in patients with TB in China. NTM species identification is very important for distinguishing between tuberculosis and NTM pulmonary diseases, and the species diversity drives the creation of diverse and integrated identification methods with higher accuracy and efficacy. Hindawi Publishing Corporation 2016 2016-11-02 /pmc/articles/PMC5110891/ /pubmed/27882322 http://dx.doi.org/10.1155/2016/2153910 Text en Copyright © 2016 Haican Liu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Liu, Haican Lian, Lulu Jiang, Yi Huang, Mingxiang Tan, Yunhong Zhao, Xiuqin Zhang, Jingrui Yu, Qin Liu, Jiao Dong, Haiyan Lu, Bing Wu, Yimou Wan, Kanglin Identification of Species of Nontuberculous Mycobacteria Clinical Isolates from 8 Provinces of China |
title | Identification of Species of Nontuberculous Mycobacteria Clinical Isolates from 8 Provinces of China |
title_full | Identification of Species of Nontuberculous Mycobacteria Clinical Isolates from 8 Provinces of China |
title_fullStr | Identification of Species of Nontuberculous Mycobacteria Clinical Isolates from 8 Provinces of China |
title_full_unstemmed | Identification of Species of Nontuberculous Mycobacteria Clinical Isolates from 8 Provinces of China |
title_short | Identification of Species of Nontuberculous Mycobacteria Clinical Isolates from 8 Provinces of China |
title_sort | identification of species of nontuberculous mycobacteria clinical isolates from 8 provinces of china |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5110891/ https://www.ncbi.nlm.nih.gov/pubmed/27882322 http://dx.doi.org/10.1155/2016/2153910 |
work_keys_str_mv | AT liuhaican identificationofspeciesofnontuberculousmycobacteriaclinicalisolatesfrom8provincesofchina AT lianlulu identificationofspeciesofnontuberculousmycobacteriaclinicalisolatesfrom8provincesofchina AT jiangyi identificationofspeciesofnontuberculousmycobacteriaclinicalisolatesfrom8provincesofchina AT huangmingxiang identificationofspeciesofnontuberculousmycobacteriaclinicalisolatesfrom8provincesofchina AT tanyunhong identificationofspeciesofnontuberculousmycobacteriaclinicalisolatesfrom8provincesofchina AT zhaoxiuqin identificationofspeciesofnontuberculousmycobacteriaclinicalisolatesfrom8provincesofchina AT zhangjingrui identificationofspeciesofnontuberculousmycobacteriaclinicalisolatesfrom8provincesofchina AT yuqin identificationofspeciesofnontuberculousmycobacteriaclinicalisolatesfrom8provincesofchina AT liujiao identificationofspeciesofnontuberculousmycobacteriaclinicalisolatesfrom8provincesofchina AT donghaiyan identificationofspeciesofnontuberculousmycobacteriaclinicalisolatesfrom8provincesofchina AT lubing identificationofspeciesofnontuberculousmycobacteriaclinicalisolatesfrom8provincesofchina AT wuyimou identificationofspeciesofnontuberculousmycobacteriaclinicalisolatesfrom8provincesofchina AT wankanglin identificationofspeciesofnontuberculousmycobacteriaclinicalisolatesfrom8provincesofchina |