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Non-invasive measurement of melanin-derived radicals in living mouse tail using X-band EPR

The aim of this experiment is to measure in vivo generation of melanin-derived radicals non-invasively, as a quantifiable index of radio-biological effect. Melanin-derived radicals in a living intact mouse tail tip were non-invasively measured in very simple way using an X-band electron paramagnetic...

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Autores principales: Ogawa, Yukihiro, Ueno, Megumi, Sekine-Suzuki, Emiko, Nakanishi, Ikuo, Matsumoto, Ken-ichiro, Fujisaki, Shingo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: the Society for Free Radical Research Japan 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5110934/
https://www.ncbi.nlm.nih.gov/pubmed/27895382
http://dx.doi.org/10.3164/jcbn.15-99
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author Ogawa, Yukihiro
Ueno, Megumi
Sekine-Suzuki, Emiko
Nakanishi, Ikuo
Matsumoto, Ken-ichiro
Fujisaki, Shingo
author_facet Ogawa, Yukihiro
Ueno, Megumi
Sekine-Suzuki, Emiko
Nakanishi, Ikuo
Matsumoto, Ken-ichiro
Fujisaki, Shingo
author_sort Ogawa, Yukihiro
collection PubMed
description The aim of this experiment is to measure in vivo generation of melanin-derived radicals non-invasively, as a quantifiable index of radio-biological effect. Melanin-derived radicals in a living intact mouse tail tip were non-invasively measured in very simple way using an X-band electron paramagnetic resonance spectrometer. Colored mouse strains, C57BL/6NCr, BDF1, and C3H/He, have clear EPR signal corresponding to melanin-derived radicals in the tail tip; however, albino mouse strains, BALB/cCr, ddY, ICR, have no EPR signals. An X-ray fraction of 2 Gy/day (1 Gy/min) was repeatedly irradiated to a C3H/He mouse tail skin every Monday to Friday for 4 weeks. In comparison to before starting irradiation, the C3H/He mouse tail skin became darker, like a suntan. The melanin-derived radicals in C3H/He mouse tail skin were increased in association with X-ray fractions. Melanin-derived radicals in mouse tail skin can be readily and chronologically measurable by using X-band EPR spectrometer, and can be a marker for a radiobiological effect in the skin.
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spelling pubmed-51109342016-11-28 Non-invasive measurement of melanin-derived radicals in living mouse tail using X-band EPR Ogawa, Yukihiro Ueno, Megumi Sekine-Suzuki, Emiko Nakanishi, Ikuo Matsumoto, Ken-ichiro Fujisaki, Shingo J Clin Biochem Nutr Original Article The aim of this experiment is to measure in vivo generation of melanin-derived radicals non-invasively, as a quantifiable index of radio-biological effect. Melanin-derived radicals in a living intact mouse tail tip were non-invasively measured in very simple way using an X-band electron paramagnetic resonance spectrometer. Colored mouse strains, C57BL/6NCr, BDF1, and C3H/He, have clear EPR signal corresponding to melanin-derived radicals in the tail tip; however, albino mouse strains, BALB/cCr, ddY, ICR, have no EPR signals. An X-ray fraction of 2 Gy/day (1 Gy/min) was repeatedly irradiated to a C3H/He mouse tail skin every Monday to Friday for 4 weeks. In comparison to before starting irradiation, the C3H/He mouse tail skin became darker, like a suntan. The melanin-derived radicals in C3H/He mouse tail skin were increased in association with X-ray fractions. Melanin-derived radicals in mouse tail skin can be readily and chronologically measurable by using X-band EPR spectrometer, and can be a marker for a radiobiological effect in the skin. the Society for Free Radical Research Japan 2016-11 2016-10-08 /pmc/articles/PMC5110934/ /pubmed/27895382 http://dx.doi.org/10.3164/jcbn.15-99 Text en Copyright © 2016 JCBN This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Ogawa, Yukihiro
Ueno, Megumi
Sekine-Suzuki, Emiko
Nakanishi, Ikuo
Matsumoto, Ken-ichiro
Fujisaki, Shingo
Non-invasive measurement of melanin-derived radicals in living mouse tail using X-band EPR
title Non-invasive measurement of melanin-derived radicals in living mouse tail using X-band EPR
title_full Non-invasive measurement of melanin-derived radicals in living mouse tail using X-band EPR
title_fullStr Non-invasive measurement of melanin-derived radicals in living mouse tail using X-band EPR
title_full_unstemmed Non-invasive measurement of melanin-derived radicals in living mouse tail using X-band EPR
title_short Non-invasive measurement of melanin-derived radicals in living mouse tail using X-band EPR
title_sort non-invasive measurement of melanin-derived radicals in living mouse tail using x-band epr
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5110934/
https://www.ncbi.nlm.nih.gov/pubmed/27895382
http://dx.doi.org/10.3164/jcbn.15-99
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