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The Mutation Profile of Calreticulin in Patients with Myeloproliferative Neoplasms and Acute Leukemia
OBJECTIVE: Calreticulin (CALR) plays important roles in cell proliferation, apoptosis, and immune responses. CALR mutations were described recently in Janus kinase 2 gene (JAK2)-negative or MPL-negative primary myelofibrosis (PMF) and essential thrombocythemia (ET) patients. CALR trails JAK2 as the...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Galenos Publishing
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5111462/ https://www.ncbi.nlm.nih.gov/pubmed/26377485 http://dx.doi.org/10.4274/tjh.2015.0220 |
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author | Wang, Jingyi Hao, Jianguo He, Na Ji, Chunyan Ma, Daoxin |
author_facet | Wang, Jingyi Hao, Jianguo He, Na Ji, Chunyan Ma, Daoxin |
author_sort | Wang, Jingyi |
collection | PubMed |
description | OBJECTIVE: Calreticulin (CALR) plays important roles in cell proliferation, apoptosis, and immune responses. CALR mutations were described recently in Janus kinase 2 gene (JAK2)-negative or MPL-negative primary myelofibrosis (PMF) and essential thrombocythemia (ET) patients. CALR trails JAK2 as the second most mutated gene in myeloproliferative neoplasms (MPNs). However, little is known about CALR mutation in Chinese patients with leukemia. In the present study, a cohort of 305 Chinese patients with hematopoietic neoplasms was screened for CALR mutations, with the aim of uncovering the frequency of CALR mutations in leukemia and MPNs. MATERIALS AND METHODS: Polymerase chain reaction and direct sequencing were performed to analyze mutations of CALR in 305 patients with hematopoietic malignancies, including 135 acute myeloid leukemia patients, 57 acute lymphoblastic leukemia patients, and 113 MPN patients. RESULTS: CALR mutations were found in 10.6% (12 of 113) of samples from patients with MPNs. CALR mutations were determined in 11.3% (6 of 53), 21.7% (5 of 23), and 9.1% (1/11) of patients with ET, PMF, and unclassifiable MPN, respectively. CONCLUSION: We showed that MPN patients carrying CALR mutations presented with higher platelet counts and lower hemoglobin levels compared to those with mutated JAK2. However, all of the leukemia patients had negative results for CALR mutations. |
format | Online Article Text |
id | pubmed-5111462 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Galenos Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-51114622016-11-21 The Mutation Profile of Calreticulin in Patients with Myeloproliferative Neoplasms and Acute Leukemia Wang, Jingyi Hao, Jianguo He, Na Ji, Chunyan Ma, Daoxin Turk J Haematol Research Article OBJECTIVE: Calreticulin (CALR) plays important roles in cell proliferation, apoptosis, and immune responses. CALR mutations were described recently in Janus kinase 2 gene (JAK2)-negative or MPL-negative primary myelofibrosis (PMF) and essential thrombocythemia (ET) patients. CALR trails JAK2 as the second most mutated gene in myeloproliferative neoplasms (MPNs). However, little is known about CALR mutation in Chinese patients with leukemia. In the present study, a cohort of 305 Chinese patients with hematopoietic neoplasms was screened for CALR mutations, with the aim of uncovering the frequency of CALR mutations in leukemia and MPNs. MATERIALS AND METHODS: Polymerase chain reaction and direct sequencing were performed to analyze mutations of CALR in 305 patients with hematopoietic malignancies, including 135 acute myeloid leukemia patients, 57 acute lymphoblastic leukemia patients, and 113 MPN patients. RESULTS: CALR mutations were found in 10.6% (12 of 113) of samples from patients with MPNs. CALR mutations were determined in 11.3% (6 of 53), 21.7% (5 of 23), and 9.1% (1/11) of patients with ET, PMF, and unclassifiable MPN, respectively. CONCLUSION: We showed that MPN patients carrying CALR mutations presented with higher platelet counts and lower hemoglobin levels compared to those with mutated JAK2. However, all of the leukemia patients had negative results for CALR mutations. Galenos Publishing 2016-09 2016-08-19 /pmc/articles/PMC5111462/ /pubmed/26377485 http://dx.doi.org/10.4274/tjh.2015.0220 Text en © Turkish Journal of Hematology, Published by Galenos Publishing. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wang, Jingyi Hao, Jianguo He, Na Ji, Chunyan Ma, Daoxin The Mutation Profile of Calreticulin in Patients with Myeloproliferative Neoplasms and Acute Leukemia |
title | The Mutation Profile of Calreticulin in Patients with Myeloproliferative Neoplasms and Acute Leukemia |
title_full | The Mutation Profile of Calreticulin in Patients with Myeloproliferative Neoplasms and Acute Leukemia |
title_fullStr | The Mutation Profile of Calreticulin in Patients with Myeloproliferative Neoplasms and Acute Leukemia |
title_full_unstemmed | The Mutation Profile of Calreticulin in Patients with Myeloproliferative Neoplasms and Acute Leukemia |
title_short | The Mutation Profile of Calreticulin in Patients with Myeloproliferative Neoplasms and Acute Leukemia |
title_sort | mutation profile of calreticulin in patients with myeloproliferative neoplasms and acute leukemia |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5111462/ https://www.ncbi.nlm.nih.gov/pubmed/26377485 http://dx.doi.org/10.4274/tjh.2015.0220 |
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