The transcriptional activator GaaR of Aspergillus niger is required for release and utilization of d‐galacturonic acid from pectin

We identified the d‐galacturonic acid (GA)‐responsive transcriptional activator GaaR of the saprotrophic fungus, Aspergillus niger, which was found to be essential for growth on GA and polygalacturonic acid (PGA). Growth of the ΔgaaR strain was reduced on complex pectins. Genome‐wide expression anal...

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Autores principales: Alazi, Ebru, Niu, Jing, Kowalczyk, Joanna E., Peng, Mao, Aguilar Pontes, Maria Victoria, van Kan, Jan A. L., Visser, Jaap, de Vries, Ronald P., Ram, Arthur F. J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Research Letters
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5111758/
https://www.ncbi.nlm.nih.gov/pubmed/27174630
http://dx.doi.org/10.1002/1873-3468.12211
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author Alazi, Ebru
Niu, Jing
Kowalczyk, Joanna E.
Peng, Mao
Aguilar Pontes, Maria Victoria
van Kan, Jan A. L.
Visser, Jaap
de Vries, Ronald P.
Ram, Arthur F. J.
author_facet Alazi, Ebru
Niu, Jing
Kowalczyk, Joanna E.
Peng, Mao
Aguilar Pontes, Maria Victoria
van Kan, Jan A. L.
Visser, Jaap
de Vries, Ronald P.
Ram, Arthur F. J.
author_sort Alazi, Ebru
collection PubMed
description We identified the d‐galacturonic acid (GA)‐responsive transcriptional activator GaaR of the saprotrophic fungus, Aspergillus niger, which was found to be essential for growth on GA and polygalacturonic acid (PGA). Growth of the ΔgaaR strain was reduced on complex pectins. Genome‐wide expression analysis showed that GaaR is required for the expression of genes necessary to release GA from PGA and more complex pectins, to transport GA into the cell, and to induce the GA catabolic pathway. Residual growth of ΔgaaR on complex pectins is likely due to the expression of pectinases acting on rhamnogalacturonan and subsequent metabolism of the monosaccharides other than GA.
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spelling pubmed-51117582016-11-16 The transcriptional activator GaaR of Aspergillus niger is required for release and utilization of d‐galacturonic acid from pectin Alazi, Ebru Niu, Jing Kowalczyk, Joanna E. Peng, Mao Aguilar Pontes, Maria Victoria van Kan, Jan A. L. Visser, Jaap de Vries, Ronald P. Ram, Arthur F. J. FEBS Lett Research Letters We identified the d‐galacturonic acid (GA)‐responsive transcriptional activator GaaR of the saprotrophic fungus, Aspergillus niger, which was found to be essential for growth on GA and polygalacturonic acid (PGA). Growth of the ΔgaaR strain was reduced on complex pectins. Genome‐wide expression analysis showed that GaaR is required for the expression of genes necessary to release GA from PGA and more complex pectins, to transport GA into the cell, and to induce the GA catabolic pathway. Residual growth of ΔgaaR on complex pectins is likely due to the expression of pectinases acting on rhamnogalacturonan and subsequent metabolism of the monosaccharides other than GA. John Wiley and Sons Inc. 2016-05-30 2016-06 /pmc/articles/PMC5111758/ /pubmed/27174630 http://dx.doi.org/10.1002/1873-3468.12211 Text en © 2016 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs (http://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Research Letters
Alazi, Ebru
Niu, Jing
Kowalczyk, Joanna E.
Peng, Mao
Aguilar Pontes, Maria Victoria
van Kan, Jan A. L.
Visser, Jaap
de Vries, Ronald P.
Ram, Arthur F. J.
The transcriptional activator GaaR of Aspergillus niger is required for release and utilization of d‐galacturonic acid from pectin
title The transcriptional activator GaaR of Aspergillus niger is required for release and utilization of d‐galacturonic acid from pectin
title_full The transcriptional activator GaaR of Aspergillus niger is required for release and utilization of d‐galacturonic acid from pectin
title_fullStr The transcriptional activator GaaR of Aspergillus niger is required for release and utilization of d‐galacturonic acid from pectin
title_full_unstemmed The transcriptional activator GaaR of Aspergillus niger is required for release and utilization of d‐galacturonic acid from pectin
title_short The transcriptional activator GaaR of Aspergillus niger is required for release and utilization of d‐galacturonic acid from pectin
title_sort transcriptional activator gaar of aspergillus niger is required for release and utilization of d‐galacturonic acid from pectin
topic Research Letters
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5111758/
https://www.ncbi.nlm.nih.gov/pubmed/27174630
http://dx.doi.org/10.1002/1873-3468.12211
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