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Postnatal regulation of MAMDC4 in the porcine intestinal epithelium is influenced by bacterial colonization

The MAM domain‐containing 4 (MAMDC4) protein is associated with the unique endocytotic mechanism observed in the intestine of mammals during the immediate postnatal period. Transcriptional expression of MAMDC4 was substantially upregulated at birth in both the piglet jejunum and ileum and its expres...

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Autores principales: Pasternak, Alex J., Hamonic, Glenn M., Van Kessel, Andrew, Wilson, Heather L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5112496/
https://www.ncbi.nlm.nih.gov/pubmed/27821716
http://dx.doi.org/10.14814/phy2.13018
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author Pasternak, Alex J.
Hamonic, Glenn M.
Van Kessel, Andrew
Wilson, Heather L.
author_facet Pasternak, Alex J.
Hamonic, Glenn M.
Van Kessel, Andrew
Wilson, Heather L.
author_sort Pasternak, Alex J.
collection PubMed
description The MAM domain‐containing 4 (MAMDC4) protein is associated with the unique endocytotic mechanism observed in the intestine of mammals during the immediate postnatal period. Transcriptional expression of MAMDC4 was substantially upregulated at birth in both the piglet jejunum and ileum and its expression decreases after birth. The protein was found localized specifically to the apical region of the luminal epithelium, however, MAMDC4 protein expression was lost at day 10 and 15 in the jejunum and ileum, respectively, and was not associated with “fetal” enterocyte replacement. Although spatial variation in the subcellular localization of Claudin 1 (CLDN1) was noted at day 3, the loss of MAMDC4 at later stages of development did not appear to have any effect on the tight junction structure. Germ‐free (GF) piglets and piglets whose gastrointestinal flora consists exclusively of Escherichia coli (EC) or Lactobacillus fermentum (LF) maintained MAMDC4 protein expression to 14 days of age in distal regions of the small intestine whereas those with conventionalized intestinal flora (CV) showed no MAMDC4 protein at this age. MAMDC4 protein expression was most pronounced in the LF and GF colonized piglets which showed staining in the epithelial cells at 75% and 95% of the length of the small intestine, respectively, which matched that of the newborn. In contrast, EC animals showed only a low abundance at these regions as well as a discontinuous staining pattern. Collectively these results suggest that maturation of MAMDC4 expression in the porcine epithelium occurs more rapidly than what is reported in previously studied rodent species. Furthermore, intestinal bacterial colonization is a major regulator of MAMDC4 in a manner specific to bacterial species and independent of enterocyte turnover.
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spelling pubmed-51124962016-11-25 Postnatal regulation of MAMDC4 in the porcine intestinal epithelium is influenced by bacterial colonization Pasternak, Alex J. Hamonic, Glenn M. Van Kessel, Andrew Wilson, Heather L. Physiol Rep Original Research The MAM domain‐containing 4 (MAMDC4) protein is associated with the unique endocytotic mechanism observed in the intestine of mammals during the immediate postnatal period. Transcriptional expression of MAMDC4 was substantially upregulated at birth in both the piglet jejunum and ileum and its expression decreases after birth. The protein was found localized specifically to the apical region of the luminal epithelium, however, MAMDC4 protein expression was lost at day 10 and 15 in the jejunum and ileum, respectively, and was not associated with “fetal” enterocyte replacement. Although spatial variation in the subcellular localization of Claudin 1 (CLDN1) was noted at day 3, the loss of MAMDC4 at later stages of development did not appear to have any effect on the tight junction structure. Germ‐free (GF) piglets and piglets whose gastrointestinal flora consists exclusively of Escherichia coli (EC) or Lactobacillus fermentum (LF) maintained MAMDC4 protein expression to 14 days of age in distal regions of the small intestine whereas those with conventionalized intestinal flora (CV) showed no MAMDC4 protein at this age. MAMDC4 protein expression was most pronounced in the LF and GF colonized piglets which showed staining in the epithelial cells at 75% and 95% of the length of the small intestine, respectively, which matched that of the newborn. In contrast, EC animals showed only a low abundance at these regions as well as a discontinuous staining pattern. Collectively these results suggest that maturation of MAMDC4 expression in the porcine epithelium occurs more rapidly than what is reported in previously studied rodent species. Furthermore, intestinal bacterial colonization is a major regulator of MAMDC4 in a manner specific to bacterial species and independent of enterocyte turnover. John Wiley and Sons Inc. 2016-11-08 /pmc/articles/PMC5112496/ /pubmed/27821716 http://dx.doi.org/10.14814/phy2.13018 Text en © 2016 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Pasternak, Alex J.
Hamonic, Glenn M.
Van Kessel, Andrew
Wilson, Heather L.
Postnatal regulation of MAMDC4 in the porcine intestinal epithelium is influenced by bacterial colonization
title Postnatal regulation of MAMDC4 in the porcine intestinal epithelium is influenced by bacterial colonization
title_full Postnatal regulation of MAMDC4 in the porcine intestinal epithelium is influenced by bacterial colonization
title_fullStr Postnatal regulation of MAMDC4 in the porcine intestinal epithelium is influenced by bacterial colonization
title_full_unstemmed Postnatal regulation of MAMDC4 in the porcine intestinal epithelium is influenced by bacterial colonization
title_short Postnatal regulation of MAMDC4 in the porcine intestinal epithelium is influenced by bacterial colonization
title_sort postnatal regulation of mamdc4 in the porcine intestinal epithelium is influenced by bacterial colonization
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5112496/
https://www.ncbi.nlm.nih.gov/pubmed/27821716
http://dx.doi.org/10.14814/phy2.13018
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