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Comparison of high-titer lactic acid fermentation from NaOH- and NH(3)-H(2)O(2)-pretreated corncob by Bacillus coagulans using simultaneous saccharification and fermentation

Lignocellulose is one of the most abundant renewable feedstocks that has attracted considerable attention as a substrate for biofuel and biochemical production. One such biochemical product, lactic acid, is an important fermentation product because of its great potential for the production of biodeg...

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Autores principales: Zhang, Zhenting, Xie, Yuejiao, He, Xiaolan, Li, Xinli, Hu, Jinlong, Ruan, Zhiyong, Zhao, Shumiao, Peng, Nan, Liang, Yunxiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5112544/
https://www.ncbi.nlm.nih.gov/pubmed/27853308
http://dx.doi.org/10.1038/srep37245
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author Zhang, Zhenting
Xie, Yuejiao
He, Xiaolan
Li, Xinli
Hu, Jinlong
Ruan, Zhiyong
Zhao, Shumiao
Peng, Nan
Liang, Yunxiang
author_facet Zhang, Zhenting
Xie, Yuejiao
He, Xiaolan
Li, Xinli
Hu, Jinlong
Ruan, Zhiyong
Zhao, Shumiao
Peng, Nan
Liang, Yunxiang
author_sort Zhang, Zhenting
collection PubMed
description Lignocellulose is one of the most abundant renewable feedstocks that has attracted considerable attention as a substrate for biofuel and biochemical production. One such biochemical product, lactic acid, is an important fermentation product because of its great potential for the production of biodegradable and biocompatible polylactic acid. High-titer lactic acid production from lignocellulosic materials has been achieved recently; however, it requires biodetoxification or results in large amounts of waste washing water. In this study, we employed two alkaline pretreatment methods and compared their effects on lactic acid fermentation of pretreated corncob by Bacillus coagulans LA204 using fed-batch simultaneous saccharification and fermentation under non-sterile conditions. The lactic acid titer, yield, and productivity from 16% (w/w) NaOH-pretreated and washed corncob were 122.99 g/L, 0.77 g/g corncob, and 1.37 g/L/h, respectively, and from 16% NH(3)-H(2)O(2)-pretreated and washed corncob were 118.60 g/L, 0.74 g/g corncob, and 1.32 g/L/h, respectively. Importantly, the lactic acid titer, yield, and productivity from 18.4% NH(3)-H(2)O(2)-pretreated and unwashed corncob by using fed-batch simultaneous saccharification and fermentation reached 79.47 g/L, 0.43 g/g corncob, and 1.10 g/L/h, respectively, demonstrating that this method is possible for industrial applications and saves washing water.
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spelling pubmed-51125442016-11-23 Comparison of high-titer lactic acid fermentation from NaOH- and NH(3)-H(2)O(2)-pretreated corncob by Bacillus coagulans using simultaneous saccharification and fermentation Zhang, Zhenting Xie, Yuejiao He, Xiaolan Li, Xinli Hu, Jinlong Ruan, Zhiyong Zhao, Shumiao Peng, Nan Liang, Yunxiang Sci Rep Article Lignocellulose is one of the most abundant renewable feedstocks that has attracted considerable attention as a substrate for biofuel and biochemical production. One such biochemical product, lactic acid, is an important fermentation product because of its great potential for the production of biodegradable and biocompatible polylactic acid. High-titer lactic acid production from lignocellulosic materials has been achieved recently; however, it requires biodetoxification or results in large amounts of waste washing water. In this study, we employed two alkaline pretreatment methods and compared their effects on lactic acid fermentation of pretreated corncob by Bacillus coagulans LA204 using fed-batch simultaneous saccharification and fermentation under non-sterile conditions. The lactic acid titer, yield, and productivity from 16% (w/w) NaOH-pretreated and washed corncob were 122.99 g/L, 0.77 g/g corncob, and 1.37 g/L/h, respectively, and from 16% NH(3)-H(2)O(2)-pretreated and washed corncob were 118.60 g/L, 0.74 g/g corncob, and 1.32 g/L/h, respectively. Importantly, the lactic acid titer, yield, and productivity from 18.4% NH(3)-H(2)O(2)-pretreated and unwashed corncob by using fed-batch simultaneous saccharification and fermentation reached 79.47 g/L, 0.43 g/g corncob, and 1.10 g/L/h, respectively, demonstrating that this method is possible for industrial applications and saves washing water. Nature Publishing Group 2016-11-17 /pmc/articles/PMC5112544/ /pubmed/27853308 http://dx.doi.org/10.1038/srep37245 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Zhang, Zhenting
Xie, Yuejiao
He, Xiaolan
Li, Xinli
Hu, Jinlong
Ruan, Zhiyong
Zhao, Shumiao
Peng, Nan
Liang, Yunxiang
Comparison of high-titer lactic acid fermentation from NaOH- and NH(3)-H(2)O(2)-pretreated corncob by Bacillus coagulans using simultaneous saccharification and fermentation
title Comparison of high-titer lactic acid fermentation from NaOH- and NH(3)-H(2)O(2)-pretreated corncob by Bacillus coagulans using simultaneous saccharification and fermentation
title_full Comparison of high-titer lactic acid fermentation from NaOH- and NH(3)-H(2)O(2)-pretreated corncob by Bacillus coagulans using simultaneous saccharification and fermentation
title_fullStr Comparison of high-titer lactic acid fermentation from NaOH- and NH(3)-H(2)O(2)-pretreated corncob by Bacillus coagulans using simultaneous saccharification and fermentation
title_full_unstemmed Comparison of high-titer lactic acid fermentation from NaOH- and NH(3)-H(2)O(2)-pretreated corncob by Bacillus coagulans using simultaneous saccharification and fermentation
title_short Comparison of high-titer lactic acid fermentation from NaOH- and NH(3)-H(2)O(2)-pretreated corncob by Bacillus coagulans using simultaneous saccharification and fermentation
title_sort comparison of high-titer lactic acid fermentation from naoh- and nh(3)-h(2)o(2)-pretreated corncob by bacillus coagulans using simultaneous saccharification and fermentation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5112544/
https://www.ncbi.nlm.nih.gov/pubmed/27853308
http://dx.doi.org/10.1038/srep37245
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