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Advances in analyzing RNA diversity in eukaryotic transcriptomes: peering through the Omics lens
Alternative splicing, polyadenylation, and chemical modifications of RNA generate astonishing complexity within eukaryotic transcriptomes. The last decade has brought numerous advances in sequencing technologies that allow biologists to investigate these phenomena with greater depth and accuracy whi...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
F1000Research
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5112568/ https://www.ncbi.nlm.nih.gov/pubmed/27909578 http://dx.doi.org/10.12688/f1000research.9511.1 |
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author | Bangru, Sushant Kalsotra, Auinash |
author_facet | Bangru, Sushant Kalsotra, Auinash |
author_sort | Bangru, Sushant |
collection | PubMed |
description | Alternative splicing, polyadenylation, and chemical modifications of RNA generate astonishing complexity within eukaryotic transcriptomes. The last decade has brought numerous advances in sequencing technologies that allow biologists to investigate these phenomena with greater depth and accuracy while reducing time and cost. A commensurate development in biochemical techniques for the enrichment and analysis of different RNA variants has accompanied the advancement of global sequencing analysis platforms. Here, we present a detailed overview of the latest biochemical methods, along with bioinformatics pipelines that have aided in identifying different RNA variants. We also highlight the ongoing developments and challenges associated with RNA variant detection and quantification, including sample heterogeneity and isolation, as well as ‘Omics’ big data handling. |
format | Online Article Text |
id | pubmed-5112568 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | F1000Research |
record_format | MEDLINE/PubMed |
spelling | pubmed-51125682016-11-30 Advances in analyzing RNA diversity in eukaryotic transcriptomes: peering through the Omics lens Bangru, Sushant Kalsotra, Auinash F1000Res Review Alternative splicing, polyadenylation, and chemical modifications of RNA generate astonishing complexity within eukaryotic transcriptomes. The last decade has brought numerous advances in sequencing technologies that allow biologists to investigate these phenomena with greater depth and accuracy while reducing time and cost. A commensurate development in biochemical techniques for the enrichment and analysis of different RNA variants has accompanied the advancement of global sequencing analysis platforms. Here, we present a detailed overview of the latest biochemical methods, along with bioinformatics pipelines that have aided in identifying different RNA variants. We also highlight the ongoing developments and challenges associated with RNA variant detection and quantification, including sample heterogeneity and isolation, as well as ‘Omics’ big data handling. F1000Research 2016-11-14 /pmc/articles/PMC5112568/ /pubmed/27909578 http://dx.doi.org/10.12688/f1000research.9511.1 Text en Copyright: © 2016 Bangru S and Kalsotra A http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Review Bangru, Sushant Kalsotra, Auinash Advances in analyzing RNA diversity in eukaryotic transcriptomes: peering through the Omics lens |
title | Advances in analyzing RNA diversity in eukaryotic transcriptomes: peering through the Omics lens |
title_full | Advances in analyzing RNA diversity in eukaryotic transcriptomes: peering through the Omics lens |
title_fullStr | Advances in analyzing RNA diversity in eukaryotic transcriptomes: peering through the Omics lens |
title_full_unstemmed | Advances in analyzing RNA diversity in eukaryotic transcriptomes: peering through the Omics lens |
title_short | Advances in analyzing RNA diversity in eukaryotic transcriptomes: peering through the Omics lens |
title_sort | advances in analyzing rna diversity in eukaryotic transcriptomes: peering through the omics lens |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5112568/ https://www.ncbi.nlm.nih.gov/pubmed/27909578 http://dx.doi.org/10.12688/f1000research.9511.1 |
work_keys_str_mv | AT bangrusushant advancesinanalyzingrnadiversityineukaryotictranscriptomespeeringthroughtheomicslens AT kalsotraauinash advancesinanalyzingrnadiversityineukaryotictranscriptomespeeringthroughtheomicslens |