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Advances in analyzing RNA diversity in eukaryotic transcriptomes: peering through the Omics lens

Alternative splicing, polyadenylation, and chemical modifications of RNA generate astonishing complexity within eukaryotic transcriptomes. The last decade has brought numerous advances in sequencing technologies that allow biologists to investigate these phenomena with greater depth and accuracy whi...

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Detalles Bibliográficos
Autores principales: Bangru, Sushant, Kalsotra, Auinash
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000Research 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5112568/
https://www.ncbi.nlm.nih.gov/pubmed/27909578
http://dx.doi.org/10.12688/f1000research.9511.1
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author Bangru, Sushant
Kalsotra, Auinash
author_facet Bangru, Sushant
Kalsotra, Auinash
author_sort Bangru, Sushant
collection PubMed
description Alternative splicing, polyadenylation, and chemical modifications of RNA generate astonishing complexity within eukaryotic transcriptomes. The last decade has brought numerous advances in sequencing technologies that allow biologists to investigate these phenomena with greater depth and accuracy while reducing time and cost. A commensurate development in biochemical techniques for the enrichment and analysis of different RNA variants has accompanied the advancement of global sequencing analysis platforms. Here, we present a detailed overview of the latest biochemical methods, along with bioinformatics pipelines that have aided in identifying different RNA variants. We also highlight the ongoing developments and challenges associated with RNA variant detection and quantification, including sample heterogeneity and isolation, as well as ‘Omics’ big data handling.
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spelling pubmed-51125682016-11-30 Advances in analyzing RNA diversity in eukaryotic transcriptomes: peering through the Omics lens Bangru, Sushant Kalsotra, Auinash F1000Res Review Alternative splicing, polyadenylation, and chemical modifications of RNA generate astonishing complexity within eukaryotic transcriptomes. The last decade has brought numerous advances in sequencing technologies that allow biologists to investigate these phenomena with greater depth and accuracy while reducing time and cost. A commensurate development in biochemical techniques for the enrichment and analysis of different RNA variants has accompanied the advancement of global sequencing analysis platforms. Here, we present a detailed overview of the latest biochemical methods, along with bioinformatics pipelines that have aided in identifying different RNA variants. We also highlight the ongoing developments and challenges associated with RNA variant detection and quantification, including sample heterogeneity and isolation, as well as ‘Omics’ big data handling. F1000Research 2016-11-14 /pmc/articles/PMC5112568/ /pubmed/27909578 http://dx.doi.org/10.12688/f1000research.9511.1 Text en Copyright: © 2016 Bangru S and Kalsotra A http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review
Bangru, Sushant
Kalsotra, Auinash
Advances in analyzing RNA diversity in eukaryotic transcriptomes: peering through the Omics lens
title Advances in analyzing RNA diversity in eukaryotic transcriptomes: peering through the Omics lens
title_full Advances in analyzing RNA diversity in eukaryotic transcriptomes: peering through the Omics lens
title_fullStr Advances in analyzing RNA diversity in eukaryotic transcriptomes: peering through the Omics lens
title_full_unstemmed Advances in analyzing RNA diversity in eukaryotic transcriptomes: peering through the Omics lens
title_short Advances in analyzing RNA diversity in eukaryotic transcriptomes: peering through the Omics lens
title_sort advances in analyzing rna diversity in eukaryotic transcriptomes: peering through the omics lens
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5112568/
https://www.ncbi.nlm.nih.gov/pubmed/27909578
http://dx.doi.org/10.12688/f1000research.9511.1
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