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Oral polymorphonuclear neutrophil characteristics in relation to oral health: a cross-sectional, observational clinical study

Polymorphonuclear neutrophils (PMNs) have a major role in the innate immune system. However, little is known about PMN contribution in relation to oral health. The objective of this study was to investigate the numbers and functional characteristics of oral PMNs (oPMNs) compared with circulatory PMN...

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Autores principales: Rijkschroeff, Patrick, Jansen, Ineke D C, van der Weijden, Fridus A, Keijser, Bart J F, Loos, Bruno G, Nicu, Elena A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5113092/
https://www.ncbi.nlm.nih.gov/pubmed/27515277
http://dx.doi.org/10.1038/ijos.2016.23
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author Rijkschroeff, Patrick
Jansen, Ineke D C
van der Weijden, Fridus A
Keijser, Bart J F
Loos, Bruno G
Nicu, Elena A
author_facet Rijkschroeff, Patrick
Jansen, Ineke D C
van der Weijden, Fridus A
Keijser, Bart J F
Loos, Bruno G
Nicu, Elena A
author_sort Rijkschroeff, Patrick
collection PubMed
description Polymorphonuclear neutrophils (PMNs) have a major role in the innate immune system. However, little is known about PMN contribution in relation to oral health. The objective of this study was to investigate the numbers and functional characteristics of oral PMNs (oPMNs) compared with circulatory PMNs (cPMNs). Oral rinse and venous blood samples were obtained from 268 systemically and orally healthy volunteers in a cross-sectional observational study. PMN counts, cell cycle analysis and cellular activation state were investigated. Also, reactive oxygen species (ROS) production was analyzed, with and without bacterial stimulation (Fusobacterium nucleatum). In males, 1.2 × 10(6)±1.0 × 10(6) oPMNs were collected, and showed a tendency to correlate with the levels of gingival bleeding (r=0.215, P=0.008). Comparable oPMNs counts were found among females (1.0 × 10(6)±0.7 × 10(6)). More late-stage apoptotic/necrotic cells were found among the oPMNs (53.1%) compared with the cPMNs (8.5% P<0.001). Without additional stimulation, oPMNs were more activated than cPMNs, as indicated by higher expression of CD11b, CD63 and CD66b, and higher constitutive ROS levels (P<0.001). Notably, in response to bacterial stimulation, oPMNs released comparable ROS levels as cPMNs (P=0.042). In conclusion, this study provides data on viable oPMNs showing high levels of activation in orally and systemically healthy individuals, free of apparent caries lesions and periodontal disease. These data suggests that although the oPMNs are in a more mature stage of their life cycle compared with the cPMNs, oPMNs are still responsive to stimulation, which indicates their functional potential and possible contribution to a healthy oral ecosystem.
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spelling pubmed-51130922016-12-06 Oral polymorphonuclear neutrophil characteristics in relation to oral health: a cross-sectional, observational clinical study Rijkschroeff, Patrick Jansen, Ineke D C van der Weijden, Fridus A Keijser, Bart J F Loos, Bruno G Nicu, Elena A Int J Oral Sci Original Article Polymorphonuclear neutrophils (PMNs) have a major role in the innate immune system. However, little is known about PMN contribution in relation to oral health. The objective of this study was to investigate the numbers and functional characteristics of oral PMNs (oPMNs) compared with circulatory PMNs (cPMNs). Oral rinse and venous blood samples were obtained from 268 systemically and orally healthy volunteers in a cross-sectional observational study. PMN counts, cell cycle analysis and cellular activation state were investigated. Also, reactive oxygen species (ROS) production was analyzed, with and without bacterial stimulation (Fusobacterium nucleatum). In males, 1.2 × 10(6)±1.0 × 10(6) oPMNs were collected, and showed a tendency to correlate with the levels of gingival bleeding (r=0.215, P=0.008). Comparable oPMNs counts were found among females (1.0 × 10(6)±0.7 × 10(6)). More late-stage apoptotic/necrotic cells were found among the oPMNs (53.1%) compared with the cPMNs (8.5% P<0.001). Without additional stimulation, oPMNs were more activated than cPMNs, as indicated by higher expression of CD11b, CD63 and CD66b, and higher constitutive ROS levels (P<0.001). Notably, in response to bacterial stimulation, oPMNs released comparable ROS levels as cPMNs (P=0.042). In conclusion, this study provides data on viable oPMNs showing high levels of activation in orally and systemically healthy individuals, free of apparent caries lesions and periodontal disease. These data suggests that although the oPMNs are in a more mature stage of their life cycle compared with the cPMNs, oPMNs are still responsive to stimulation, which indicates their functional potential and possible contribution to a healthy oral ecosystem. Nature Publishing Group 2016-09 2016-08-12 /pmc/articles/PMC5113092/ /pubmed/27515277 http://dx.doi.org/10.1038/ijos.2016.23 Text en Copyright © 2016 International Journal of Oral Science http://creativecommons.org/licenses/by-nc-sa/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/
spellingShingle Original Article
Rijkschroeff, Patrick
Jansen, Ineke D C
van der Weijden, Fridus A
Keijser, Bart J F
Loos, Bruno G
Nicu, Elena A
Oral polymorphonuclear neutrophil characteristics in relation to oral health: a cross-sectional, observational clinical study
title Oral polymorphonuclear neutrophil characteristics in relation to oral health: a cross-sectional, observational clinical study
title_full Oral polymorphonuclear neutrophil characteristics in relation to oral health: a cross-sectional, observational clinical study
title_fullStr Oral polymorphonuclear neutrophil characteristics in relation to oral health: a cross-sectional, observational clinical study
title_full_unstemmed Oral polymorphonuclear neutrophil characteristics in relation to oral health: a cross-sectional, observational clinical study
title_short Oral polymorphonuclear neutrophil characteristics in relation to oral health: a cross-sectional, observational clinical study
title_sort oral polymorphonuclear neutrophil characteristics in relation to oral health: a cross-sectional, observational clinical study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5113092/
https://www.ncbi.nlm.nih.gov/pubmed/27515277
http://dx.doi.org/10.1038/ijos.2016.23
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