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Scarcity of autoreactive human blood IgA(+) memory B cells
Class‐switched memory B cells are key components of the “reactive” humoral immunity, which ensures a fast and massive secretion of high‐affinity antigen‐specific antibodies upon antigenic challenge. In humans, IgA class‐switched (IgA(+)) memory B cells and IgA antibodies are abundant in the blood. A...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5113776/ https://www.ncbi.nlm.nih.gov/pubmed/27469325 http://dx.doi.org/10.1002/eji.201646446 |
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author | Prigent, Julie Lorin, Valérie Kök, Ayrin Hieu, Thierry Bourgeau, Salomé Mouquet, Hugo |
author_facet | Prigent, Julie Lorin, Valérie Kök, Ayrin Hieu, Thierry Bourgeau, Salomé Mouquet, Hugo |
author_sort | Prigent, Julie |
collection | PubMed |
description | Class‐switched memory B cells are key components of the “reactive” humoral immunity, which ensures a fast and massive secretion of high‐affinity antigen‐specific antibodies upon antigenic challenge. In humans, IgA class‐switched (IgA(+)) memory B cells and IgA antibodies are abundant in the blood. Although circulating IgA(+) memory B cells and their corresponding secreted immunoglobulins likely possess major protective and/or regulatory immune roles, little is known about their specificity and function. Here, we show that IgA(+) and IgG(+) memory B‐cell antibodies cloned from the same healthy humans share common immunoglobulin gene features. IgA and IgG memory antibodies have comparable lack of reactivity to vaccines, common mucosa‐tropic viruses and commensal bacteria. However, the IgA(+) memory B‐cell compartment contains fewer polyreactive clones and importantly, only rare self‐reactive clones compared to IgG(+) memory B cells. Self‐reactivity of IgAs is acquired following B‐cell affinity maturation but not antibody class switching. Together, our data suggest the existence of different regulatory mechanisms for removing autoreactive clones from the IgG(+) and IgA(+) memory B‐cell repertoires, and/or different maturation pathways potentially reflecting the distinct nature and localization of the cognate antigens recognized by individual B‐cell populations. |
format | Online Article Text |
id | pubmed-5113776 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-51137762016-12-02 Scarcity of autoreactive human blood IgA(+) memory B cells Prigent, Julie Lorin, Valérie Kök, Ayrin Hieu, Thierry Bourgeau, Salomé Mouquet, Hugo Eur J Immunol Adaptive immunity Class‐switched memory B cells are key components of the “reactive” humoral immunity, which ensures a fast and massive secretion of high‐affinity antigen‐specific antibodies upon antigenic challenge. In humans, IgA class‐switched (IgA(+)) memory B cells and IgA antibodies are abundant in the blood. Although circulating IgA(+) memory B cells and their corresponding secreted immunoglobulins likely possess major protective and/or regulatory immune roles, little is known about their specificity and function. Here, we show that IgA(+) and IgG(+) memory B‐cell antibodies cloned from the same healthy humans share common immunoglobulin gene features. IgA and IgG memory antibodies have comparable lack of reactivity to vaccines, common mucosa‐tropic viruses and commensal bacteria. However, the IgA(+) memory B‐cell compartment contains fewer polyreactive clones and importantly, only rare self‐reactive clones compared to IgG(+) memory B cells. Self‐reactivity of IgAs is acquired following B‐cell affinity maturation but not antibody class switching. Together, our data suggest the existence of different regulatory mechanisms for removing autoreactive clones from the IgG(+) and IgA(+) memory B‐cell repertoires, and/or different maturation pathways potentially reflecting the distinct nature and localization of the cognate antigens recognized by individual B‐cell populations. John Wiley and Sons Inc. 2016-08-25 2016-10 /pmc/articles/PMC5113776/ /pubmed/27469325 http://dx.doi.org/10.1002/eji.201646446 Text en © 2016 The Authors. European Journal of Immunology published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs (http://creativecommons.org/licenses/by-nc-nd/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Adaptive immunity Prigent, Julie Lorin, Valérie Kök, Ayrin Hieu, Thierry Bourgeau, Salomé Mouquet, Hugo Scarcity of autoreactive human blood IgA(+) memory B cells |
title | Scarcity of autoreactive human blood IgA(+) memory B cells |
title_full | Scarcity of autoreactive human blood IgA(+) memory B cells |
title_fullStr | Scarcity of autoreactive human blood IgA(+) memory B cells |
title_full_unstemmed | Scarcity of autoreactive human blood IgA(+) memory B cells |
title_short | Scarcity of autoreactive human blood IgA(+) memory B cells |
title_sort | scarcity of autoreactive human blood iga(+) memory b cells |
topic | Adaptive immunity |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5113776/ https://www.ncbi.nlm.nih.gov/pubmed/27469325 http://dx.doi.org/10.1002/eji.201646446 |
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