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Analysis of alternative lengthening of telomere markers in BRCA1 defective cells
Telomeres are specialized structures responsible for the chromosome end protection. Previous studies have revealed that defective BRCA1 may lead to elevated telomere fusions and accelerated telomere shortening. In addition, BRCA1 associates with promyelocytic leukemia (PML) bodies in alternative len...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5113789/ https://www.ncbi.nlm.nih.gov/pubmed/27295426 http://dx.doi.org/10.1002/gcc.22386 |
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author | Kargaran, Parisa K. Yasaei, Hemad Anjomani‐Virmouni, Sara Mangiapane, Giovanna Slijepcevic, Predrag |
author_facet | Kargaran, Parisa K. Yasaei, Hemad Anjomani‐Virmouni, Sara Mangiapane, Giovanna Slijepcevic, Predrag |
author_sort | Kargaran, Parisa K. |
collection | PubMed |
description | Telomeres are specialized structures responsible for the chromosome end protection. Previous studies have revealed that defective BRCA1 may lead to elevated telomere fusions and accelerated telomere shortening. In addition, BRCA1 associates with promyelocytic leukemia (PML) bodies in alternative lengthening of telomeres (ALTs) positive cells. We report here elevated recombination rates at telomeres in cells from human BRCA1 mutation carriers and in mouse embryonic stem cells lacking both copies of functional Brca1. An increased recombination rate at telomeres is one of the signs of ALT. To investigate this possibility further we employed the C‐circle assay that identifies ALT unequivocally. Our results revealed elevated levels of ALT activity in Brca1 defective mouse cells. Similar results were obtained when the same cells were assayed for the presence of another ALT marker, namely the frequency of PML bodies. These results suggest that BRCA1 may act as a repressor of ALT. © 2016 The Authors Genes, Chromosomes & Cancer Published by Wiley Periodicals, Inc. |
format | Online Article Text |
id | pubmed-5113789 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-51137892016-12-02 Analysis of alternative lengthening of telomere markers in BRCA1 defective cells Kargaran, Parisa K. Yasaei, Hemad Anjomani‐Virmouni, Sara Mangiapane, Giovanna Slijepcevic, Predrag Genes Chromosomes Cancer Research Articles Telomeres are specialized structures responsible for the chromosome end protection. Previous studies have revealed that defective BRCA1 may lead to elevated telomere fusions and accelerated telomere shortening. In addition, BRCA1 associates with promyelocytic leukemia (PML) bodies in alternative lengthening of telomeres (ALTs) positive cells. We report here elevated recombination rates at telomeres in cells from human BRCA1 mutation carriers and in mouse embryonic stem cells lacking both copies of functional Brca1. An increased recombination rate at telomeres is one of the signs of ALT. To investigate this possibility further we employed the C‐circle assay that identifies ALT unequivocally. Our results revealed elevated levels of ALT activity in Brca1 defective mouse cells. Similar results were obtained when the same cells were assayed for the presence of another ALT marker, namely the frequency of PML bodies. These results suggest that BRCA1 may act as a repressor of ALT. © 2016 The Authors Genes, Chromosomes & Cancer Published by Wiley Periodicals, Inc. John Wiley and Sons Inc. 2016-07-26 2016-11 /pmc/articles/PMC5113789/ /pubmed/27295426 http://dx.doi.org/10.1002/gcc.22386 Text en © 2016 The Authors Genes, Chromosomes & Cancer Published by Wiley Periodicals, Inc. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs (http://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Research Articles Kargaran, Parisa K. Yasaei, Hemad Anjomani‐Virmouni, Sara Mangiapane, Giovanna Slijepcevic, Predrag Analysis of alternative lengthening of telomere markers in BRCA1 defective cells |
title | Analysis of alternative lengthening of telomere markers in BRCA1 defective cells |
title_full | Analysis of alternative lengthening of telomere markers in BRCA1 defective cells |
title_fullStr | Analysis of alternative lengthening of telomere markers in BRCA1 defective cells |
title_full_unstemmed | Analysis of alternative lengthening of telomere markers in BRCA1 defective cells |
title_short | Analysis of alternative lengthening of telomere markers in BRCA1 defective cells |
title_sort | analysis of alternative lengthening of telomere markers in brca1 defective cells |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5113789/ https://www.ncbi.nlm.nih.gov/pubmed/27295426 http://dx.doi.org/10.1002/gcc.22386 |
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