Cargando…

Spatial organization of astrocytes in ferret visual cortex

Astrocytes form an intricate partnership with neural circuits to influence numerous cellular and synaptic processes. One prominent organizational feature of astrocytes is the “tiling” of the brain with non‐overlapping territories. There are some documented species and brain region–specific astrocyte...

Descripción completa

Detalles Bibliográficos
Autores principales: López‐Hidalgo, Mónica, Hoover, Walter B., Schummers, James
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5113791/
https://www.ncbi.nlm.nih.gov/pubmed/27072916
http://dx.doi.org/10.1002/cne.24015
_version_ 1782468246120693760
author López‐Hidalgo, Mónica
Hoover, Walter B.
Schummers, James
author_facet López‐Hidalgo, Mónica
Hoover, Walter B.
Schummers, James
author_sort López‐Hidalgo, Mónica
collection PubMed
description Astrocytes form an intricate partnership with neural circuits to influence numerous cellular and synaptic processes. One prominent organizational feature of astrocytes is the “tiling” of the brain with non‐overlapping territories. There are some documented species and brain region–specific astrocyte specializations, but the extent of astrocyte diversity and circuit specificity are still unknown. We quantitatively defined the rules that govern the spatial arrangement of astrocyte somata and territory overlap in ferret visual cortex using a combination of in vivo two‐photon imaging, morphological reconstruction, immunostaining, and model simulations. We found that ferret astrocytes share, on average, half of their territory with other astrocytes. However, a specific class of astrocytes, abundant in thalamo‐recipient cortical layers (“kissing” astrocytes), overlap markedly less. Together, these results demonstrate novel features of astrocyte organization indicating that different classes of astrocytes are arranged in a circuit‐specific manner and that tiling does not apply universally across brain regions and species. J. Comp. Neurol. 524:3561–3576, 2016. © 2016 The Authors The Journal of Comparative Neurology Published by Wiley Periodicals, Inc.
format Online
Article
Text
id pubmed-5113791
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-51137912016-12-02 Spatial organization of astrocytes in ferret visual cortex López‐Hidalgo, Mónica Hoover, Walter B. Schummers, James J Comp Neurol Research Articles Astrocytes form an intricate partnership with neural circuits to influence numerous cellular and synaptic processes. One prominent organizational feature of astrocytes is the “tiling” of the brain with non‐overlapping territories. There are some documented species and brain region–specific astrocyte specializations, but the extent of astrocyte diversity and circuit specificity are still unknown. We quantitatively defined the rules that govern the spatial arrangement of astrocyte somata and territory overlap in ferret visual cortex using a combination of in vivo two‐photon imaging, morphological reconstruction, immunostaining, and model simulations. We found that ferret astrocytes share, on average, half of their territory with other astrocytes. However, a specific class of astrocytes, abundant in thalamo‐recipient cortical layers (“kissing” astrocytes), overlap markedly less. Together, these results demonstrate novel features of astrocyte organization indicating that different classes of astrocytes are arranged in a circuit‐specific manner and that tiling does not apply universally across brain regions and species. J. Comp. Neurol. 524:3561–3576, 2016. © 2016 The Authors The Journal of Comparative Neurology Published by Wiley Periodicals, Inc. John Wiley and Sons Inc. 2016-08-11 2016-12-01 /pmc/articles/PMC5113791/ /pubmed/27072916 http://dx.doi.org/10.1002/cne.24015 Text en © 2016 The Authors The Journal of Comparative Neurology Published by Wiley Periodicals, Inc. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial (http://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Research Articles
López‐Hidalgo, Mónica
Hoover, Walter B.
Schummers, James
Spatial organization of astrocytes in ferret visual cortex
title Spatial organization of astrocytes in ferret visual cortex
title_full Spatial organization of astrocytes in ferret visual cortex
title_fullStr Spatial organization of astrocytes in ferret visual cortex
title_full_unstemmed Spatial organization of astrocytes in ferret visual cortex
title_short Spatial organization of astrocytes in ferret visual cortex
title_sort spatial organization of astrocytes in ferret visual cortex
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5113791/
https://www.ncbi.nlm.nih.gov/pubmed/27072916
http://dx.doi.org/10.1002/cne.24015
work_keys_str_mv AT lopezhidalgomonica spatialorganizationofastrocytesinferretvisualcortex
AT hooverwalterb spatialorganizationofastrocytesinferretvisualcortex
AT schummersjames spatialorganizationofastrocytesinferretvisualcortex