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Isolation and Characterization of Acinetobacter baumannii Recovered from Campylobacter Selective Medium

Acinetobacter baumannii, a Gram-negative opportunistic pathogen, is known to cause multidrug resistant infections. This organism has primarily been isolated from clinical environments and its environmental reservoirs remain largely unknown. In the present study, we recovered seven isolates of A. bau...

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Autores principales: Fernando, Dinesh M., Khan, Izhar U. H., Patidar, Rakesh, Lapen, David R., Talbot, Guylaine, Topp, Edward, Kumar, Ayush
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5114274/
https://www.ncbi.nlm.nih.gov/pubmed/27917170
http://dx.doi.org/10.3389/fmicb.2016.01871
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author Fernando, Dinesh M.
Khan, Izhar U. H.
Patidar, Rakesh
Lapen, David R.
Talbot, Guylaine
Topp, Edward
Kumar, Ayush
author_facet Fernando, Dinesh M.
Khan, Izhar U. H.
Patidar, Rakesh
Lapen, David R.
Talbot, Guylaine
Topp, Edward
Kumar, Ayush
author_sort Fernando, Dinesh M.
collection PubMed
description Acinetobacter baumannii, a Gram-negative opportunistic pathogen, is known to cause multidrug resistant infections. This organism has primarily been isolated from clinical environments and its environmental reservoirs remain largely unknown. In the present study, we recovered seven isolates of A. baumannii growing under conditions selective for Campylobacter spp. (microaerophilic at 42°C and in the presence of antibiotics) from dairy cattle manure storage tank or surface water impacted by livestock effluents. Antibiotic susceptibility tests revealed that all of these isolates were less susceptible to at least two different clinically relevant antibiotics, compared to the type strain A. baumannii ATCC17978. Expression of resistance-nodulation-division efflux pumps, an important mechanism of intrinsic resistance in these organisms, was analyzed, and adeB was found to be overexpressed in one and adeJ was overexpressed in three isolates. Comparison of these isolates using genomic DNA Macro-Restriction Fragment Pattern Analysis (MRFPA) revealed relatively low relatedness among themselves or with some of the clinical isolates from previous studies. This study suggests that A. baumannii isolates are capable of growing under selective conditions for Campylobacter spp. and that this organism can be present in manure and water.
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spelling pubmed-51142742016-12-02 Isolation and Characterization of Acinetobacter baumannii Recovered from Campylobacter Selective Medium Fernando, Dinesh M. Khan, Izhar U. H. Patidar, Rakesh Lapen, David R. Talbot, Guylaine Topp, Edward Kumar, Ayush Front Microbiol Microbiology Acinetobacter baumannii, a Gram-negative opportunistic pathogen, is known to cause multidrug resistant infections. This organism has primarily been isolated from clinical environments and its environmental reservoirs remain largely unknown. In the present study, we recovered seven isolates of A. baumannii growing under conditions selective for Campylobacter spp. (microaerophilic at 42°C and in the presence of antibiotics) from dairy cattle manure storage tank or surface water impacted by livestock effluents. Antibiotic susceptibility tests revealed that all of these isolates were less susceptible to at least two different clinically relevant antibiotics, compared to the type strain A. baumannii ATCC17978. Expression of resistance-nodulation-division efflux pumps, an important mechanism of intrinsic resistance in these organisms, was analyzed, and adeB was found to be overexpressed in one and adeJ was overexpressed in three isolates. Comparison of these isolates using genomic DNA Macro-Restriction Fragment Pattern Analysis (MRFPA) revealed relatively low relatedness among themselves or with some of the clinical isolates from previous studies. This study suggests that A. baumannii isolates are capable of growing under selective conditions for Campylobacter spp. and that this organism can be present in manure and water. Frontiers Media S.A. 2016-11-18 /pmc/articles/PMC5114274/ /pubmed/27917170 http://dx.doi.org/10.3389/fmicb.2016.01871 Text en Copyright © 2016 Fernando, Khan, Patidar, Lapen, Talbot, Topp and Kumar. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Fernando, Dinesh M.
Khan, Izhar U. H.
Patidar, Rakesh
Lapen, David R.
Talbot, Guylaine
Topp, Edward
Kumar, Ayush
Isolation and Characterization of Acinetobacter baumannii Recovered from Campylobacter Selective Medium
title Isolation and Characterization of Acinetobacter baumannii Recovered from Campylobacter Selective Medium
title_full Isolation and Characterization of Acinetobacter baumannii Recovered from Campylobacter Selective Medium
title_fullStr Isolation and Characterization of Acinetobacter baumannii Recovered from Campylobacter Selective Medium
title_full_unstemmed Isolation and Characterization of Acinetobacter baumannii Recovered from Campylobacter Selective Medium
title_short Isolation and Characterization of Acinetobacter baumannii Recovered from Campylobacter Selective Medium
title_sort isolation and characterization of acinetobacter baumannii recovered from campylobacter selective medium
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5114274/
https://www.ncbi.nlm.nih.gov/pubmed/27917170
http://dx.doi.org/10.3389/fmicb.2016.01871
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