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Preventive effects of kilohertz frequency electrical stimulation on sepsis-induced muscle atrophy

OBJECTIVES: The present study sought to evaluate the effect of electrical stimulation (ES) by using kilohertz frequency on muscle atrophy induced by sepsis. METHODS: Seventeen male ICR mice were randomly divided into 3 groups: control, lipopolysaccharide (LPS)-injected for 4 days, LPS plus ES (LPS+E...

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Autores principales: Tanaka, M., Tanaka, K., Tategaki, J., Fujino, H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Society of Musculoskeletal and Neuronal Interactions 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5114358/
https://www.ncbi.nlm.nih.gov/pubmed/27282459
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author Tanaka, M.
Tanaka, K.
Tategaki, J.
Fujino, H.
author_facet Tanaka, M.
Tanaka, K.
Tategaki, J.
Fujino, H.
author_sort Tanaka, M.
collection PubMed
description OBJECTIVES: The present study sought to evaluate the effect of electrical stimulation (ES) by using kilohertz frequency on muscle atrophy induced by sepsis. METHODS: Seventeen male ICR mice were randomly divided into 3 groups: control, lipopolysaccharide (LPS)-injected for 4 days, LPS plus ES (LPS+ES). Sepsis was induced by 4 days of an intraperitoneal LPS injection (10 µg/g body weight/day). LPS+ES animals received the LPS injections and ES twice a day for 4 days. ELISA and western blot analysis determined the plasma levels of inflammatory cytokines and ubiquitinated proteins, while the tibialis anterior muscles were weighed and muscle fiber cross-sectional area (CSA) were measured to assess muscle atrophy, which were analyzed by Student’s t-test and ANOVA. RESULTS: LPS induced increased plasma levels of inflammatory cytokines, significant muscle mass loss (LPS:-29.0%, LPS+ES:-23.1%), decreased fiber cross-sectional area, and an up-regulation of atrogin-1 and ubiquitinated proteins in the tibialis anterior muscle compared with the control. ES attenuated the sepsis-induced loss of muscle mass and decreased fiber CSA, as well as attenuated the atrogin-1 and ubiquitinated protein up-regulation. CONCLUSIONS: Electrical stimulation may prevent sepsis-induced muscle atrophy through ubiquitin-proteasome pathway inhibition.
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spelling pubmed-51143582016-11-21 Preventive effects of kilohertz frequency electrical stimulation on sepsis-induced muscle atrophy Tanaka, M. Tanaka, K. Tategaki, J. Fujino, H. J Musculoskelet Neuronal Interact Original Article OBJECTIVES: The present study sought to evaluate the effect of electrical stimulation (ES) by using kilohertz frequency on muscle atrophy induced by sepsis. METHODS: Seventeen male ICR mice were randomly divided into 3 groups: control, lipopolysaccharide (LPS)-injected for 4 days, LPS plus ES (LPS+ES). Sepsis was induced by 4 days of an intraperitoneal LPS injection (10 µg/g body weight/day). LPS+ES animals received the LPS injections and ES twice a day for 4 days. ELISA and western blot analysis determined the plasma levels of inflammatory cytokines and ubiquitinated proteins, while the tibialis anterior muscles were weighed and muscle fiber cross-sectional area (CSA) were measured to assess muscle atrophy, which were analyzed by Student’s t-test and ANOVA. RESULTS: LPS induced increased plasma levels of inflammatory cytokines, significant muscle mass loss (LPS:-29.0%, LPS+ES:-23.1%), decreased fiber cross-sectional area, and an up-regulation of atrogin-1 and ubiquitinated proteins in the tibialis anterior muscle compared with the control. ES attenuated the sepsis-induced loss of muscle mass and decreased fiber CSA, as well as attenuated the atrogin-1 and ubiquitinated protein up-regulation. CONCLUSIONS: Electrical stimulation may prevent sepsis-induced muscle atrophy through ubiquitin-proteasome pathway inhibition. International Society of Musculoskeletal and Neuronal Interactions 2016-06 /pmc/articles/PMC5114358/ /pubmed/27282459 Text en Copyright: © Journal of Musculoskeletal and Neuronal Interactions http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Tanaka, M.
Tanaka, K.
Tategaki, J.
Fujino, H.
Preventive effects of kilohertz frequency electrical stimulation on sepsis-induced muscle atrophy
title Preventive effects of kilohertz frequency electrical stimulation on sepsis-induced muscle atrophy
title_full Preventive effects of kilohertz frequency electrical stimulation on sepsis-induced muscle atrophy
title_fullStr Preventive effects of kilohertz frequency electrical stimulation on sepsis-induced muscle atrophy
title_full_unstemmed Preventive effects of kilohertz frequency electrical stimulation on sepsis-induced muscle atrophy
title_short Preventive effects of kilohertz frequency electrical stimulation on sepsis-induced muscle atrophy
title_sort preventive effects of kilohertz frequency electrical stimulation on sepsis-induced muscle atrophy
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5114358/
https://www.ncbi.nlm.nih.gov/pubmed/27282459
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