Complete Nucleotide Sequence of an Australian Isolate of Turnip mosaic virus before and after Seven Years of Serial Passaging

The complete genome sequence of an Australian isolate of Turnip mosaic virus was determined by Sanger sequencing. After seven years of serial passaging by mechanical inoculation, the isolate was resequenced by RNA sequencing (RNA-Seq). Eighteen single nucleotide polymorphisms were identified between...

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Autores principales: Pretorius, Lara, Moyle, Richard L., Dalton-Morgan, Jessica, Hussein, Nasser, Schenk, Peer M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2016
Materias:
Viruses
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5114374/
https://www.ncbi.nlm.nih.gov/pubmed/27856582
http://dx.doi.org/10.1128/genomeA.01269-16
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author Pretorius, Lara
Moyle, Richard L.
Dalton-Morgan, Jessica
Hussein, Nasser
Schenk, Peer M.
author_facet Pretorius, Lara
Moyle, Richard L.
Dalton-Morgan, Jessica
Hussein, Nasser
Schenk, Peer M.
author_sort Pretorius, Lara
collection PubMed
description The complete genome sequence of an Australian isolate of Turnip mosaic virus was determined by Sanger sequencing. After seven years of serial passaging by mechanical inoculation, the isolate was resequenced by RNA sequencing (RNA-Seq). Eighteen single nucleotide polymorphisms were identified between the isolates. Both isolates had 96% identity to isolate AUST10.
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spelling pubmed-51143742016-11-30 Complete Nucleotide Sequence of an Australian Isolate of Turnip mosaic virus before and after Seven Years of Serial Passaging Pretorius, Lara Moyle, Richard L. Dalton-Morgan, Jessica Hussein, Nasser Schenk, Peer M. Genome Announc Viruses The complete genome sequence of an Australian isolate of Turnip mosaic virus was determined by Sanger sequencing. After seven years of serial passaging by mechanical inoculation, the isolate was resequenced by RNA sequencing (RNA-Seq). Eighteen single nucleotide polymorphisms were identified between the isolates. Both isolates had 96% identity to isolate AUST10. American Society for Microbiology 2016-11-17 /pmc/articles/PMC5114374/ /pubmed/27856582 http://dx.doi.org/10.1128/genomeA.01269-16 Text en Copyright © 2016 Pretorius et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Viruses
Pretorius, Lara
Moyle, Richard L.
Dalton-Morgan, Jessica
Hussein, Nasser
Schenk, Peer M.
Complete Nucleotide Sequence of an Australian Isolate of Turnip mosaic virus before and after Seven Years of Serial Passaging
title Complete Nucleotide Sequence of an Australian Isolate of Turnip mosaic virus before and after Seven Years of Serial Passaging
title_full Complete Nucleotide Sequence of an Australian Isolate of Turnip mosaic virus before and after Seven Years of Serial Passaging
title_fullStr Complete Nucleotide Sequence of an Australian Isolate of Turnip mosaic virus before and after Seven Years of Serial Passaging
title_full_unstemmed Complete Nucleotide Sequence of an Australian Isolate of Turnip mosaic virus before and after Seven Years of Serial Passaging
title_short Complete Nucleotide Sequence of an Australian Isolate of Turnip mosaic virus before and after Seven Years of Serial Passaging
title_sort complete nucleotide sequence of an australian isolate of turnip mosaic virus before and after seven years of serial passaging
topic Viruses
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5114374/
https://www.ncbi.nlm.nih.gov/pubmed/27856582
http://dx.doi.org/10.1128/genomeA.01269-16
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