Application of target capture sequencing of exons and conserved non-coding sequences to 20 inbred rat strains

We report sequence data obtained by our recently devised target capture method TargetEC applied to 20 inbred rat strains. This method encompasses not only all annotated exons but also highly conserved non-coding sequences shared among vertebrates. The total length of the target regions covers 146.8 ...

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Autores principales: Yoshihara, Minako, Sato, Tetsuya, Saito, Daisuke, Ohara, Osamu, Kuramoto, Takashi, Suyama, Mikita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Data in Brief
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5114524/
https://www.ncbi.nlm.nih.gov/pubmed/27882299
http://dx.doi.org/10.1016/j.gdata.2016.11.010
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author Yoshihara, Minako
Sato, Tetsuya
Saito, Daisuke
Ohara, Osamu
Kuramoto, Takashi
Suyama, Mikita
author_facet Yoshihara, Minako
Sato, Tetsuya
Saito, Daisuke
Ohara, Osamu
Kuramoto, Takashi
Suyama, Mikita
author_sort Yoshihara, Minako
collection PubMed
description We report sequence data obtained by our recently devised target capture method TargetEC applied to 20 inbred rat strains. This method encompasses not only all annotated exons but also highly conserved non-coding sequences shared among vertebrates. The total length of the target regions covers 146.8 Mb. On an average, we obtained 31.7 × depth of target coverage and identified 154,330 SNVs and 24,368 INDELs for each strain. This corresponds to 470,037 unique SNVs and 68,652 unique INDELs among the 20 strains. The sequence data can be accessed at DDBJ/EMBL/GenBank under accession number PRJDB4648, and the identified variants have been deposited at http://bioinfo.sls.kyushu-u.ac.jp/rat_target_capture/20_strains.vcf.gz.
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spelling pubmed-51145242016-11-23 Application of target capture sequencing of exons and conserved non-coding sequences to 20 inbred rat strains Yoshihara, Minako Sato, Tetsuya Saito, Daisuke Ohara, Osamu Kuramoto, Takashi Suyama, Mikita Genom Data Data in Brief We report sequence data obtained by our recently devised target capture method TargetEC applied to 20 inbred rat strains. This method encompasses not only all annotated exons but also highly conserved non-coding sequences shared among vertebrates. The total length of the target regions covers 146.8 Mb. On an average, we obtained 31.7 × depth of target coverage and identified 154,330 SNVs and 24,368 INDELs for each strain. This corresponds to 470,037 unique SNVs and 68,652 unique INDELs among the 20 strains. The sequence data can be accessed at DDBJ/EMBL/GenBank under accession number PRJDB4648, and the identified variants have been deposited at http://bioinfo.sls.kyushu-u.ac.jp/rat_target_capture/20_strains.vcf.gz. Elsevier 2016-11-14 /pmc/articles/PMC5114524/ /pubmed/27882299 http://dx.doi.org/10.1016/j.gdata.2016.11.010 Text en © 2016 Stellenbosch University http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Data in Brief
Yoshihara, Minako
Sato, Tetsuya
Saito, Daisuke
Ohara, Osamu
Kuramoto, Takashi
Suyama, Mikita
Application of target capture sequencing of exons and conserved non-coding sequences to 20 inbred rat strains
title Application of target capture sequencing of exons and conserved non-coding sequences to 20 inbred rat strains
title_full Application of target capture sequencing of exons and conserved non-coding sequences to 20 inbred rat strains
title_fullStr Application of target capture sequencing of exons and conserved non-coding sequences to 20 inbred rat strains
title_full_unstemmed Application of target capture sequencing of exons and conserved non-coding sequences to 20 inbred rat strains
title_short Application of target capture sequencing of exons and conserved non-coding sequences to 20 inbred rat strains
title_sort application of target capture sequencing of exons and conserved non-coding sequences to 20 inbred rat strains
topic Data in Brief
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5114524/
https://www.ncbi.nlm.nih.gov/pubmed/27882299
http://dx.doi.org/10.1016/j.gdata.2016.11.010
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