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Cloning and characterization of TaVIP2 gene from Triticum aestivum and functional analysis in Nicotiana tabacum
Wheat is recalcitrant to genetic transformation. A potential solution is to manipulate the expression of some host proteins involved in T-DNA integration process. VirE2 interacting protein 2 (VIP2) plays an important role in T-DNA transport and integration. In this study, a TaVIP2 gene was cloned fr...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5114603/ https://www.ncbi.nlm.nih.gov/pubmed/27857194 http://dx.doi.org/10.1038/srep37602 |
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author | Zhao, Pei Wang, Ke Lin, Zhishan Zhang, Wei Du, Lipu Zhang, Yunlong Ye, Xingguo |
author_facet | Zhao, Pei Wang, Ke Lin, Zhishan Zhang, Wei Du, Lipu Zhang, Yunlong Ye, Xingguo |
author_sort | Zhao, Pei |
collection | PubMed |
description | Wheat is recalcitrant to genetic transformation. A potential solution is to manipulate the expression of some host proteins involved in T-DNA integration process. VirE2 interacting protein 2 (VIP2) plays an important role in T-DNA transport and integration. In this study, a TaVIP2 gene was cloned from common wheat. Southern blot and allele-specific polymerase chain reaction (AS-PCR) combined with an online chromosomal location software tool revealed that three TaVIP2 genes were located on wheat chromosomes 1AL, 1BL, and 1DL. These three homoeoallelic TaVIP2 genes all contained 13 exons and 12 introns, and their coding sequences were the same; there were a few single nucleotide polymorphisms (SNPs) among the three genes. The heterologous expression of the TaVIP2 gene in tobacco led to enhancement of the Agrobacterium-mediated transformation efficiency up to 2.5-fold. Transgenic tobacco plants expressing TaVIP2 showed enhanced resistance to powdery mildew. Further quantitative real-time PCR (qRT-PCR) revealed that overexpression of TaVIP2 in transgenic tobacco up-regulated the expression of an endogenous gene, NtPR-1, which likely contributed to powdery mildew resistance in transgenic tobacco. Our study indicates that the TaVIP2 gene may be highly useful in efforts to improve Agrobacterium-mediated transformation efficiency and to enhance powdery mildew resistance in wheat. |
format | Online Article Text |
id | pubmed-5114603 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-51146032016-11-25 Cloning and characterization of TaVIP2 gene from Triticum aestivum and functional analysis in Nicotiana tabacum Zhao, Pei Wang, Ke Lin, Zhishan Zhang, Wei Du, Lipu Zhang, Yunlong Ye, Xingguo Sci Rep Article Wheat is recalcitrant to genetic transformation. A potential solution is to manipulate the expression of some host proteins involved in T-DNA integration process. VirE2 interacting protein 2 (VIP2) plays an important role in T-DNA transport and integration. In this study, a TaVIP2 gene was cloned from common wheat. Southern blot and allele-specific polymerase chain reaction (AS-PCR) combined with an online chromosomal location software tool revealed that three TaVIP2 genes were located on wheat chromosomes 1AL, 1BL, and 1DL. These three homoeoallelic TaVIP2 genes all contained 13 exons and 12 introns, and their coding sequences were the same; there were a few single nucleotide polymorphisms (SNPs) among the three genes. The heterologous expression of the TaVIP2 gene in tobacco led to enhancement of the Agrobacterium-mediated transformation efficiency up to 2.5-fold. Transgenic tobacco plants expressing TaVIP2 showed enhanced resistance to powdery mildew. Further quantitative real-time PCR (qRT-PCR) revealed that overexpression of TaVIP2 in transgenic tobacco up-regulated the expression of an endogenous gene, NtPR-1, which likely contributed to powdery mildew resistance in transgenic tobacco. Our study indicates that the TaVIP2 gene may be highly useful in efforts to improve Agrobacterium-mediated transformation efficiency and to enhance powdery mildew resistance in wheat. Nature Publishing Group 2016-11-18 /pmc/articles/PMC5114603/ /pubmed/27857194 http://dx.doi.org/10.1038/srep37602 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Zhao, Pei Wang, Ke Lin, Zhishan Zhang, Wei Du, Lipu Zhang, Yunlong Ye, Xingguo Cloning and characterization of TaVIP2 gene from Triticum aestivum and functional analysis in Nicotiana tabacum |
title | Cloning and characterization of TaVIP2 gene from Triticum aestivum and functional analysis in Nicotiana tabacum |
title_full | Cloning and characterization of TaVIP2 gene from Triticum aestivum and functional analysis in Nicotiana tabacum |
title_fullStr | Cloning and characterization of TaVIP2 gene from Triticum aestivum and functional analysis in Nicotiana tabacum |
title_full_unstemmed | Cloning and characterization of TaVIP2 gene from Triticum aestivum and functional analysis in Nicotiana tabacum |
title_short | Cloning and characterization of TaVIP2 gene from Triticum aestivum and functional analysis in Nicotiana tabacum |
title_sort | cloning and characterization of tavip2 gene from triticum aestivum and functional analysis in nicotiana tabacum |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5114603/ https://www.ncbi.nlm.nih.gov/pubmed/27857194 http://dx.doi.org/10.1038/srep37602 |
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