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Nanochannel Electroporation as a Platform for Living Cell Interrogation in Acute Myeloid Leukemia

A living cell interrogation platform based on nanochannel electroporation is demonstrated with analysis of RNAs in single cells. This minimally invasive process is based on individual cells and allows both multi‐target analysis and stimulus‐response analysis by sequential deliveries. The unique plat...

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Autores principales: Zhao, Xi, Huang, Xiaomeng, Wang, Xinmei, Wu, Yun, Eisfeld, Ann‐Kathrin, Schwind, Sebastian, Gallego‐Perez, Daniel, Boukany, Pouyan E., Marcucci, Guido I., Lee, Ly James
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5115302/
https://www.ncbi.nlm.nih.gov/pubmed/27980918
http://dx.doi.org/10.1002/advs.201500111
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author Zhao, Xi
Huang, Xiaomeng
Wang, Xinmei
Wu, Yun
Eisfeld, Ann‐Kathrin
Schwind, Sebastian
Gallego‐Perez, Daniel
Boukany, Pouyan E.
Marcucci, Guido I.
Lee, Ly James
author_facet Zhao, Xi
Huang, Xiaomeng
Wang, Xinmei
Wu, Yun
Eisfeld, Ann‐Kathrin
Schwind, Sebastian
Gallego‐Perez, Daniel
Boukany, Pouyan E.
Marcucci, Guido I.
Lee, Ly James
author_sort Zhao, Xi
collection PubMed
description A living cell interrogation platform based on nanochannel electroporation is demonstrated with analysis of RNAs in single cells. This minimally invasive process is based on individual cells and allows both multi‐target analysis and stimulus‐response analysis by sequential deliveries. The unique platform possesses a great potential to the comprehensive and lysis‐free nucleic acid analysis on rare or hard‐to‐transfect cells. [Image: see text]
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spelling pubmed-51153022016-12-15 Nanochannel Electroporation as a Platform for Living Cell Interrogation in Acute Myeloid Leukemia Zhao, Xi Huang, Xiaomeng Wang, Xinmei Wu, Yun Eisfeld, Ann‐Kathrin Schwind, Sebastian Gallego‐Perez, Daniel Boukany, Pouyan E. Marcucci, Guido I. Lee, Ly James Adv Sci (Weinh) Communications A living cell interrogation platform based on nanochannel electroporation is demonstrated with analysis of RNAs in single cells. This minimally invasive process is based on individual cells and allows both multi‐target analysis and stimulus‐response analysis by sequential deliveries. The unique platform possesses a great potential to the comprehensive and lysis‐free nucleic acid analysis on rare or hard‐to‐transfect cells. [Image: see text] John Wiley and Sons Inc. 2015-07-16 /pmc/articles/PMC5115302/ /pubmed/27980918 http://dx.doi.org/10.1002/advs.201500111 Text en © 2015 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Communications
Zhao, Xi
Huang, Xiaomeng
Wang, Xinmei
Wu, Yun
Eisfeld, Ann‐Kathrin
Schwind, Sebastian
Gallego‐Perez, Daniel
Boukany, Pouyan E.
Marcucci, Guido I.
Lee, Ly James
Nanochannel Electroporation as a Platform for Living Cell Interrogation in Acute Myeloid Leukemia
title Nanochannel Electroporation as a Platform for Living Cell Interrogation in Acute Myeloid Leukemia
title_full Nanochannel Electroporation as a Platform for Living Cell Interrogation in Acute Myeloid Leukemia
title_fullStr Nanochannel Electroporation as a Platform for Living Cell Interrogation in Acute Myeloid Leukemia
title_full_unstemmed Nanochannel Electroporation as a Platform for Living Cell Interrogation in Acute Myeloid Leukemia
title_short Nanochannel Electroporation as a Platform for Living Cell Interrogation in Acute Myeloid Leukemia
title_sort nanochannel electroporation as a platform for living cell interrogation in acute myeloid leukemia
topic Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5115302/
https://www.ncbi.nlm.nih.gov/pubmed/27980918
http://dx.doi.org/10.1002/advs.201500111
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