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Transcriptome Profiling of Watermelon Root in Response to Short-Term Osmotic Stress

Osmotic stress adversely affects the growth, fruit quality and yield of watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai). Increasing the tolerance of watermelon to osmotic stress caused by factors such as high salt and water deficit is an effective way to improve crop survival in osmotic...

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Autores principales: Yang, Yongchao, Mo, Yanling, Yang, Xiaozheng, Zhang, Haifei, Wang, Yongqi, Li, Hao, Wei, Chunhua, Zhang, Xian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5115733/
https://www.ncbi.nlm.nih.gov/pubmed/27861528
http://dx.doi.org/10.1371/journal.pone.0166314
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author Yang, Yongchao
Mo, Yanling
Yang, Xiaozheng
Zhang, Haifei
Wang, Yongqi
Li, Hao
Wei, Chunhua
Zhang, Xian
author_facet Yang, Yongchao
Mo, Yanling
Yang, Xiaozheng
Zhang, Haifei
Wang, Yongqi
Li, Hao
Wei, Chunhua
Zhang, Xian
author_sort Yang, Yongchao
collection PubMed
description Osmotic stress adversely affects the growth, fruit quality and yield of watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai). Increasing the tolerance of watermelon to osmotic stress caused by factors such as high salt and water deficit is an effective way to improve crop survival in osmotic stress environments. Roots are important organs in water absorption and are involved in the initial response to osmosis stress; however, few studies have examined the underlying mechanism of tolerance to osmotic stress in watermelon roots. For better understanding of this mechanism, the inbred watermelon accession M08, which exhibits relatively high tolerance to water deficits, was treated with 20% polyethylene glycol (PEG) 6000. The root samples were harvested at 6 h after PEG treatment and untreated samples were used as controls. Transcriptome analyses were carried out by Illumina RNA sequencing. A total of 5246 differentially expressed genes were identified. Gene ontology enrichment and biochemical pathway analyses of these 5246 genes showed that short-term osmotic stress affected osmotic adjustment, signal transduction, hormone responses, cell division, cell cycle and ribosome, and M08 may repress root growth to adapt osmotic stress. The results of this study describe the watermelon root transcriptome under osmotic stress and propose new insight into watermelon root responses to osmotic stress at the transcriptome level. Accordingly, these results allow us to better understand the molecular mechanisms of watermelon in response to drought stress and will facilitate watermelon breeding projects to improve drought tolerance.
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spelling pubmed-51157332016-12-08 Transcriptome Profiling of Watermelon Root in Response to Short-Term Osmotic Stress Yang, Yongchao Mo, Yanling Yang, Xiaozheng Zhang, Haifei Wang, Yongqi Li, Hao Wei, Chunhua Zhang, Xian PLoS One Research Article Osmotic stress adversely affects the growth, fruit quality and yield of watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai). Increasing the tolerance of watermelon to osmotic stress caused by factors such as high salt and water deficit is an effective way to improve crop survival in osmotic stress environments. Roots are important organs in water absorption and are involved in the initial response to osmosis stress; however, few studies have examined the underlying mechanism of tolerance to osmotic stress in watermelon roots. For better understanding of this mechanism, the inbred watermelon accession M08, which exhibits relatively high tolerance to water deficits, was treated with 20% polyethylene glycol (PEG) 6000. The root samples were harvested at 6 h after PEG treatment and untreated samples were used as controls. Transcriptome analyses were carried out by Illumina RNA sequencing. A total of 5246 differentially expressed genes were identified. Gene ontology enrichment and biochemical pathway analyses of these 5246 genes showed that short-term osmotic stress affected osmotic adjustment, signal transduction, hormone responses, cell division, cell cycle and ribosome, and M08 may repress root growth to adapt osmotic stress. The results of this study describe the watermelon root transcriptome under osmotic stress and propose new insight into watermelon root responses to osmotic stress at the transcriptome level. Accordingly, these results allow us to better understand the molecular mechanisms of watermelon in response to drought stress and will facilitate watermelon breeding projects to improve drought tolerance. Public Library of Science 2016-11-18 /pmc/articles/PMC5115733/ /pubmed/27861528 http://dx.doi.org/10.1371/journal.pone.0166314 Text en © 2016 Yang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Yang, Yongchao
Mo, Yanling
Yang, Xiaozheng
Zhang, Haifei
Wang, Yongqi
Li, Hao
Wei, Chunhua
Zhang, Xian
Transcriptome Profiling of Watermelon Root in Response to Short-Term Osmotic Stress
title Transcriptome Profiling of Watermelon Root in Response to Short-Term Osmotic Stress
title_full Transcriptome Profiling of Watermelon Root in Response to Short-Term Osmotic Stress
title_fullStr Transcriptome Profiling of Watermelon Root in Response to Short-Term Osmotic Stress
title_full_unstemmed Transcriptome Profiling of Watermelon Root in Response to Short-Term Osmotic Stress
title_short Transcriptome Profiling of Watermelon Root in Response to Short-Term Osmotic Stress
title_sort transcriptome profiling of watermelon root in response to short-term osmotic stress
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5115733/
https://www.ncbi.nlm.nih.gov/pubmed/27861528
http://dx.doi.org/10.1371/journal.pone.0166314
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