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Extraocular motoneuron pools develop along a dorsoventral axis in zebrafish, Danio rerio

Both spatial and temporal cues determine the fate of immature neurons. A major challenge at the interface of developmental and systems neuroscience is to relate this spatiotemporal trajectory of maturation to circuit‐level functional organization. This study examined the development of two extraocul...

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Autores principales: Greaney, Marie R., Privorotskiy, Ann E., D'Elia, Kristen P., Schoppik, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5116274/
https://www.ncbi.nlm.nih.gov/pubmed/27197595
http://dx.doi.org/10.1002/cne.24042
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author Greaney, Marie R.
Privorotskiy, Ann E.
D'Elia, Kristen P.
Schoppik, David
author_facet Greaney, Marie R.
Privorotskiy, Ann E.
D'Elia, Kristen P.
Schoppik, David
author_sort Greaney, Marie R.
collection PubMed
description Both spatial and temporal cues determine the fate of immature neurons. A major challenge at the interface of developmental and systems neuroscience is to relate this spatiotemporal trajectory of maturation to circuit‐level functional organization. This study examined the development of two extraocular motor nuclei (nIII and nIV), structures in which a motoneuron's identity, or choice of muscle partner, defines its behavioral role. We used retro‐orbital dye fills, in combination with fluorescent markers for motoneuron location and birthdate, to probe spatial and temporal organization of the oculomotor (nIII) and trochlear (nIV) nuclei in the larval zebrafish. We describe a dorsoventral organization of the four nIII motoneuron pools, in which inferior and medial rectus motoneurons occupy dorsal nIII, while inferior oblique and superior rectus motoneurons occupy distinct divisions of ventral nIII. Dorsal nIII motoneurons are, moreover, born before motoneurons of ventral nIII and nIV. The order of neurogenesis can therefore account for the dorsoventral organization of nIII and may play a primary role in determining motoneuron identity. We propose that the temporal development of extraocular motoneurons plays a key role in assembling a functional oculomotor circuit. J. Comp. Neurol. 525:65–78, 2017. © 2016 The Authors The Journal of Comparative Neurology Published by Wiley Periodicals, Inc.
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spelling pubmed-51162742016-12-02 Extraocular motoneuron pools develop along a dorsoventral axis in zebrafish, Danio rerio Greaney, Marie R. Privorotskiy, Ann E. D'Elia, Kristen P. Schoppik, David J Comp Neurol Research Articles Both spatial and temporal cues determine the fate of immature neurons. A major challenge at the interface of developmental and systems neuroscience is to relate this spatiotemporal trajectory of maturation to circuit‐level functional organization. This study examined the development of two extraocular motor nuclei (nIII and nIV), structures in which a motoneuron's identity, or choice of muscle partner, defines its behavioral role. We used retro‐orbital dye fills, in combination with fluorescent markers for motoneuron location and birthdate, to probe spatial and temporal organization of the oculomotor (nIII) and trochlear (nIV) nuclei in the larval zebrafish. We describe a dorsoventral organization of the four nIII motoneuron pools, in which inferior and medial rectus motoneurons occupy dorsal nIII, while inferior oblique and superior rectus motoneurons occupy distinct divisions of ventral nIII. Dorsal nIII motoneurons are, moreover, born before motoneurons of ventral nIII and nIV. The order of neurogenesis can therefore account for the dorsoventral organization of nIII and may play a primary role in determining motoneuron identity. We propose that the temporal development of extraocular motoneurons plays a key role in assembling a functional oculomotor circuit. J. Comp. Neurol. 525:65–78, 2017. © 2016 The Authors The Journal of Comparative Neurology Published by Wiley Periodicals, Inc. John Wiley and Sons Inc. 2016-06-01 2017-01-01 /pmc/articles/PMC5116274/ /pubmed/27197595 http://dx.doi.org/10.1002/cne.24042 Text en © 2016 The Authors The Journal of Comparative Neurology Published by Wiley Periodicals, Inc. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Greaney, Marie R.
Privorotskiy, Ann E.
D'Elia, Kristen P.
Schoppik, David
Extraocular motoneuron pools develop along a dorsoventral axis in zebrafish, Danio rerio
title Extraocular motoneuron pools develop along a dorsoventral axis in zebrafish, Danio rerio
title_full Extraocular motoneuron pools develop along a dorsoventral axis in zebrafish, Danio rerio
title_fullStr Extraocular motoneuron pools develop along a dorsoventral axis in zebrafish, Danio rerio
title_full_unstemmed Extraocular motoneuron pools develop along a dorsoventral axis in zebrafish, Danio rerio
title_short Extraocular motoneuron pools develop along a dorsoventral axis in zebrafish, Danio rerio
title_sort extraocular motoneuron pools develop along a dorsoventral axis in zebrafish, danio rerio
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5116274/
https://www.ncbi.nlm.nih.gov/pubmed/27197595
http://dx.doi.org/10.1002/cne.24042
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