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Suppression of Rac1 Signaling by Influenza A Virus NS1 Facilitates Viral Replication

Influenza A virus (IAV) is a major human pathogen with the potential to become pandemic. IAV contains only eight RNA segments; thus, the virus must fully exploit the host cellular machinery to facilitate its own replication. In an effort to comprehensively characterize the host machinery taken over...

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Autores principales: Jiang, Wei, Sheng, Chunjie, Gu, Xiuling, Liu, Dong, Yao, Chen, Gao, Shijuan, Chen, Shuai, Huang, Yinghui, Huang, Wenlin, Fang, Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5116764/
https://www.ncbi.nlm.nih.gov/pubmed/27869202
http://dx.doi.org/10.1038/srep35041
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author Jiang, Wei
Sheng, Chunjie
Gu, Xiuling
Liu, Dong
Yao, Chen
Gao, Shijuan
Chen, Shuai
Huang, Yinghui
Huang, Wenlin
Fang, Min
author_facet Jiang, Wei
Sheng, Chunjie
Gu, Xiuling
Liu, Dong
Yao, Chen
Gao, Shijuan
Chen, Shuai
Huang, Yinghui
Huang, Wenlin
Fang, Min
author_sort Jiang, Wei
collection PubMed
description Influenza A virus (IAV) is a major human pathogen with the potential to become pandemic. IAV contains only eight RNA segments; thus, the virus must fully exploit the host cellular machinery to facilitate its own replication. In an effort to comprehensively characterize the host machinery taken over by IAV in mammalian cells, we generated stable A549 cell lines with over-expression of the viral non-structural protein (NS1) to investigate the potential host factors that might be modulated by the NS1 protein. We found that the viral NS1 protein directly interacted with cellular Rac1 and facilitated viral replication. Further research revealed that NS1 down-regulated Rac1 activity via post-translational modifications. Therefore, our results demonstrated that IAV blocked Rac1-mediated host cell signal transduction through the NS1 protein to facilitate its own replication. Our findings provide a novel insight into the mechanism of IAV replication and indicate new avenues for the development of potential therapeutic targets.
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spelling pubmed-51167642016-11-28 Suppression of Rac1 Signaling by Influenza A Virus NS1 Facilitates Viral Replication Jiang, Wei Sheng, Chunjie Gu, Xiuling Liu, Dong Yao, Chen Gao, Shijuan Chen, Shuai Huang, Yinghui Huang, Wenlin Fang, Min Sci Rep Article Influenza A virus (IAV) is a major human pathogen with the potential to become pandemic. IAV contains only eight RNA segments; thus, the virus must fully exploit the host cellular machinery to facilitate its own replication. In an effort to comprehensively characterize the host machinery taken over by IAV in mammalian cells, we generated stable A549 cell lines with over-expression of the viral non-structural protein (NS1) to investigate the potential host factors that might be modulated by the NS1 protein. We found that the viral NS1 protein directly interacted with cellular Rac1 and facilitated viral replication. Further research revealed that NS1 down-regulated Rac1 activity via post-translational modifications. Therefore, our results demonstrated that IAV blocked Rac1-mediated host cell signal transduction through the NS1 protein to facilitate its own replication. Our findings provide a novel insight into the mechanism of IAV replication and indicate new avenues for the development of potential therapeutic targets. Nature Publishing Group 2016-11-21 /pmc/articles/PMC5116764/ /pubmed/27869202 http://dx.doi.org/10.1038/srep35041 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Jiang, Wei
Sheng, Chunjie
Gu, Xiuling
Liu, Dong
Yao, Chen
Gao, Shijuan
Chen, Shuai
Huang, Yinghui
Huang, Wenlin
Fang, Min
Suppression of Rac1 Signaling by Influenza A Virus NS1 Facilitates Viral Replication
title Suppression of Rac1 Signaling by Influenza A Virus NS1 Facilitates Viral Replication
title_full Suppression of Rac1 Signaling by Influenza A Virus NS1 Facilitates Viral Replication
title_fullStr Suppression of Rac1 Signaling by Influenza A Virus NS1 Facilitates Viral Replication
title_full_unstemmed Suppression of Rac1 Signaling by Influenza A Virus NS1 Facilitates Viral Replication
title_short Suppression of Rac1 Signaling by Influenza A Virus NS1 Facilitates Viral Replication
title_sort suppression of rac1 signaling by influenza a virus ns1 facilitates viral replication
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5116764/
https://www.ncbi.nlm.nih.gov/pubmed/27869202
http://dx.doi.org/10.1038/srep35041
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