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Immunodetection of NETs in Paraffin-Embedded Tissue

The pathogenic potential of neutrophil extracellular traps (NETs) was recently described, and their detection in tissue could serve as a prognostic marker. NETs are delicate and filigree structures; hence good tissue preservation is essential for their detection. Indeed, analysis of paraffin-embedde...

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Autores principales: Brinkmann, Volker, Abu Abed, Ulrike, Goosmann, Christian, Zychlinsky, Arturo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5118445/
https://www.ncbi.nlm.nih.gov/pubmed/27920776
http://dx.doi.org/10.3389/fimmu.2016.00513
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author Brinkmann, Volker
Abu Abed, Ulrike
Goosmann, Christian
Zychlinsky, Arturo
author_facet Brinkmann, Volker
Abu Abed, Ulrike
Goosmann, Christian
Zychlinsky, Arturo
author_sort Brinkmann, Volker
collection PubMed
description The pathogenic potential of neutrophil extracellular traps (NETs) was recently described, and their detection in tissue could serve as a prognostic marker. NETs are delicate and filigree structures; hence good tissue preservation is essential for their detection. Indeed, analysis of paraffin-embedded tissue has proven superior to the study of cryo sections. Though, under favorable conditions, the presence of NETs can be detected in tissue sections stained with histological dyes, definitive identification of NETs needs the colocalization of immunofluorescent signals for both nuclear and granular (or cytoplasmic) NET components. We tested diverse antigen retrieval methods and various combinations of commercially available antibodies and present here staining protocols to detect NETs in human and murine tissue sections.
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spelling pubmed-51184452016-12-05 Immunodetection of NETs in Paraffin-Embedded Tissue Brinkmann, Volker Abu Abed, Ulrike Goosmann, Christian Zychlinsky, Arturo Front Immunol Immunology The pathogenic potential of neutrophil extracellular traps (NETs) was recently described, and their detection in tissue could serve as a prognostic marker. NETs are delicate and filigree structures; hence good tissue preservation is essential for their detection. Indeed, analysis of paraffin-embedded tissue has proven superior to the study of cryo sections. Though, under favorable conditions, the presence of NETs can be detected in tissue sections stained with histological dyes, definitive identification of NETs needs the colocalization of immunofluorescent signals for both nuclear and granular (or cytoplasmic) NET components. We tested diverse antigen retrieval methods and various combinations of commercially available antibodies and present here staining protocols to detect NETs in human and murine tissue sections. Frontiers Media S.A. 2016-11-22 /pmc/articles/PMC5118445/ /pubmed/27920776 http://dx.doi.org/10.3389/fimmu.2016.00513 Text en Copyright © 2016 Brinkmann, Abu Abed, Goosmann and Zychlinsky. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Brinkmann, Volker
Abu Abed, Ulrike
Goosmann, Christian
Zychlinsky, Arturo
Immunodetection of NETs in Paraffin-Embedded Tissue
title Immunodetection of NETs in Paraffin-Embedded Tissue
title_full Immunodetection of NETs in Paraffin-Embedded Tissue
title_fullStr Immunodetection of NETs in Paraffin-Embedded Tissue
title_full_unstemmed Immunodetection of NETs in Paraffin-Embedded Tissue
title_short Immunodetection of NETs in Paraffin-Embedded Tissue
title_sort immunodetection of nets in paraffin-embedded tissue
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5118445/
https://www.ncbi.nlm.nih.gov/pubmed/27920776
http://dx.doi.org/10.3389/fimmu.2016.00513
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