Cargando…

Advances in Imaging Techniques and Genetically Encoded Probes for Photoacoustic Imaging

Photoacoustic (PA) imaging is a rapidly emerging biomedical imaging modality that is capable of visualizing cellular and molecular functions with high detection sensitivity and spatial resolution in deep tissue. Great efforts and progress have been made on the development of various PA imaging techn...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Chengbo, Gong, Xiaojing, Lin, Riqiang, Liu, Feng, Chen, Jingqin, Wang, Zhiyong, Song, Liang, Chu, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5118604/
https://www.ncbi.nlm.nih.gov/pubmed/27877244
http://dx.doi.org/10.7150/thno.15878
_version_ 1782468957575315456
author Liu, Chengbo
Gong, Xiaojing
Lin, Riqiang
Liu, Feng
Chen, Jingqin
Wang, Zhiyong
Song, Liang
Chu, Jun
author_facet Liu, Chengbo
Gong, Xiaojing
Lin, Riqiang
Liu, Feng
Chen, Jingqin
Wang, Zhiyong
Song, Liang
Chu, Jun
author_sort Liu, Chengbo
collection PubMed
description Photoacoustic (PA) imaging is a rapidly emerging biomedical imaging modality that is capable of visualizing cellular and molecular functions with high detection sensitivity and spatial resolution in deep tissue. Great efforts and progress have been made on the development of various PA imaging technologies with improved resolution and sensitivity over the past two decades. Various PA probes with high contrast have also been extensively developed, with many important biomedical applications. In comparison with chemical dyes and nanoparticles, genetically encoded probes offer easier labeling of defined cells within tissues or proteins of interest within a cell, have higher stability in vivo, and eliminate the need for delivery of exogenous substances. Genetically encoded probes have thus attracted increasing attention from researchers in engineering and biomedicine. In this review, we aim to provide an overview of the existing PA imaging technologies and genetically encoded PA probes, and describe further improvements in PA imaging techniques and the near-infrared photochromic protein BphP1, the most sensitive genetically encoded probe thus far, as well as the potential biomedical applications of BphP1-based PA imaging in vivo.
format Online
Article
Text
id pubmed-5118604
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Ivyspring International Publisher
record_format MEDLINE/PubMed
spelling pubmed-51186042016-11-22 Advances in Imaging Techniques and Genetically Encoded Probes for Photoacoustic Imaging Liu, Chengbo Gong, Xiaojing Lin, Riqiang Liu, Feng Chen, Jingqin Wang, Zhiyong Song, Liang Chu, Jun Theranostics Review Photoacoustic (PA) imaging is a rapidly emerging biomedical imaging modality that is capable of visualizing cellular and molecular functions with high detection sensitivity and spatial resolution in deep tissue. Great efforts and progress have been made on the development of various PA imaging technologies with improved resolution and sensitivity over the past two decades. Various PA probes with high contrast have also been extensively developed, with many important biomedical applications. In comparison with chemical dyes and nanoparticles, genetically encoded probes offer easier labeling of defined cells within tissues or proteins of interest within a cell, have higher stability in vivo, and eliminate the need for delivery of exogenous substances. Genetically encoded probes have thus attracted increasing attention from researchers in engineering and biomedicine. In this review, we aim to provide an overview of the existing PA imaging technologies and genetically encoded PA probes, and describe further improvements in PA imaging techniques and the near-infrared photochromic protein BphP1, the most sensitive genetically encoded probe thus far, as well as the potential biomedical applications of BphP1-based PA imaging in vivo. Ivyspring International Publisher 2016-10-07 /pmc/articles/PMC5118604/ /pubmed/27877244 http://dx.doi.org/10.7150/thno.15878 Text en © Ivyspring International Publisher. Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited. See http://ivyspring.com/terms for terms and conditions.
spellingShingle Review
Liu, Chengbo
Gong, Xiaojing
Lin, Riqiang
Liu, Feng
Chen, Jingqin
Wang, Zhiyong
Song, Liang
Chu, Jun
Advances in Imaging Techniques and Genetically Encoded Probes for Photoacoustic Imaging
title Advances in Imaging Techniques and Genetically Encoded Probes for Photoacoustic Imaging
title_full Advances in Imaging Techniques and Genetically Encoded Probes for Photoacoustic Imaging
title_fullStr Advances in Imaging Techniques and Genetically Encoded Probes for Photoacoustic Imaging
title_full_unstemmed Advances in Imaging Techniques and Genetically Encoded Probes for Photoacoustic Imaging
title_short Advances in Imaging Techniques and Genetically Encoded Probes for Photoacoustic Imaging
title_sort advances in imaging techniques and genetically encoded probes for photoacoustic imaging
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5118604/
https://www.ncbi.nlm.nih.gov/pubmed/27877244
http://dx.doi.org/10.7150/thno.15878
work_keys_str_mv AT liuchengbo advancesinimagingtechniquesandgeneticallyencodedprobesforphotoacousticimaging
AT gongxiaojing advancesinimagingtechniquesandgeneticallyencodedprobesforphotoacousticimaging
AT linriqiang advancesinimagingtechniquesandgeneticallyencodedprobesforphotoacousticimaging
AT liufeng advancesinimagingtechniquesandgeneticallyencodedprobesforphotoacousticimaging
AT chenjingqin advancesinimagingtechniquesandgeneticallyencodedprobesforphotoacousticimaging
AT wangzhiyong advancesinimagingtechniquesandgeneticallyencodedprobesforphotoacousticimaging
AT songliang advancesinimagingtechniquesandgeneticallyencodedprobesforphotoacousticimaging
AT chujun advancesinimagingtechniquesandgeneticallyencodedprobesforphotoacousticimaging