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Natural zwitterionic betaine enables cells to survive ultrarapid cryopreservation
Cryoprotectants (CPAs) play a critical role in cryopreservation because they can resist the cell damage caused by the freezing process. Current state-of-the-art CPAs are mainly based on an organic solvent dimethyl sulfoxide (DMSO), and several DMSO-cryopreserved cell products have been brought to ma...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5118695/ https://www.ncbi.nlm.nih.gov/pubmed/27874036 http://dx.doi.org/10.1038/srep37458 |
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author | Yang, Jing Cai, Nana Zhai, Hongwen Zhang, Jiamin Zhu, Yingnan Zhang, Lei |
author_facet | Yang, Jing Cai, Nana Zhai, Hongwen Zhang, Jiamin Zhu, Yingnan Zhang, Lei |
author_sort | Yang, Jing |
collection | PubMed |
description | Cryoprotectants (CPAs) play a critical role in cryopreservation because they can resist the cell damage caused by the freezing process. Current state-of-the-art CPAs are mainly based on an organic solvent dimethyl sulfoxide (DMSO), and several DMSO-cryopreserved cell products have been brought to market. However, the intrinsic toxicity and complex freezing protocol of DMSO still remain as the bottleneck of the wide use for clinical applications. Herein, we reported that betaine, a natural zwitterionic molecule, could serve as a nontoxic and high efficient CPA. At optimum concentration of betaine, different cell types exhibited exceptional post-thaw survival efficiency with ultrarapid freezing protocol, which was straightforward, cost efficient but difficult to succeed using DMSO. Moreover, betaine showed negligible cytotoxicity even after long-term exposure of cells. Mechanistically, we hypothesized that betaine could be ultra-rapidly taken up by cells for intracellular protection during the freezing process. This technology unlocks the possibility of alternating the traditional toxic CPAs and is applicable to a variety of clinical applications. |
format | Online Article Text |
id | pubmed-5118695 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-51186952016-11-28 Natural zwitterionic betaine enables cells to survive ultrarapid cryopreservation Yang, Jing Cai, Nana Zhai, Hongwen Zhang, Jiamin Zhu, Yingnan Zhang, Lei Sci Rep Article Cryoprotectants (CPAs) play a critical role in cryopreservation because they can resist the cell damage caused by the freezing process. Current state-of-the-art CPAs are mainly based on an organic solvent dimethyl sulfoxide (DMSO), and several DMSO-cryopreserved cell products have been brought to market. However, the intrinsic toxicity and complex freezing protocol of DMSO still remain as the bottleneck of the wide use for clinical applications. Herein, we reported that betaine, a natural zwitterionic molecule, could serve as a nontoxic and high efficient CPA. At optimum concentration of betaine, different cell types exhibited exceptional post-thaw survival efficiency with ultrarapid freezing protocol, which was straightforward, cost efficient but difficult to succeed using DMSO. Moreover, betaine showed negligible cytotoxicity even after long-term exposure of cells. Mechanistically, we hypothesized that betaine could be ultra-rapidly taken up by cells for intracellular protection during the freezing process. This technology unlocks the possibility of alternating the traditional toxic CPAs and is applicable to a variety of clinical applications. Nature Publishing Group 2016-11-22 /pmc/articles/PMC5118695/ /pubmed/27874036 http://dx.doi.org/10.1038/srep37458 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Yang, Jing Cai, Nana Zhai, Hongwen Zhang, Jiamin Zhu, Yingnan Zhang, Lei Natural zwitterionic betaine enables cells to survive ultrarapid cryopreservation |
title | Natural zwitterionic betaine enables cells to survive ultrarapid cryopreservation |
title_full | Natural zwitterionic betaine enables cells to survive ultrarapid cryopreservation |
title_fullStr | Natural zwitterionic betaine enables cells to survive ultrarapid cryopreservation |
title_full_unstemmed | Natural zwitterionic betaine enables cells to survive ultrarapid cryopreservation |
title_short | Natural zwitterionic betaine enables cells to survive ultrarapid cryopreservation |
title_sort | natural zwitterionic betaine enables cells to survive ultrarapid cryopreservation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5118695/ https://www.ncbi.nlm.nih.gov/pubmed/27874036 http://dx.doi.org/10.1038/srep37458 |
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