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Natural zwitterionic betaine enables cells to survive ultrarapid cryopreservation

Cryoprotectants (CPAs) play a critical role in cryopreservation because they can resist the cell damage caused by the freezing process. Current state-of-the-art CPAs are mainly based on an organic solvent dimethyl sulfoxide (DMSO), and several DMSO-cryopreserved cell products have been brought to ma...

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Autores principales: Yang, Jing, Cai, Nana, Zhai, Hongwen, Zhang, Jiamin, Zhu, Yingnan, Zhang, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5118695/
https://www.ncbi.nlm.nih.gov/pubmed/27874036
http://dx.doi.org/10.1038/srep37458
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author Yang, Jing
Cai, Nana
Zhai, Hongwen
Zhang, Jiamin
Zhu, Yingnan
Zhang, Lei
author_facet Yang, Jing
Cai, Nana
Zhai, Hongwen
Zhang, Jiamin
Zhu, Yingnan
Zhang, Lei
author_sort Yang, Jing
collection PubMed
description Cryoprotectants (CPAs) play a critical role in cryopreservation because they can resist the cell damage caused by the freezing process. Current state-of-the-art CPAs are mainly based on an organic solvent dimethyl sulfoxide (DMSO), and several DMSO-cryopreserved cell products have been brought to market. However, the intrinsic toxicity and complex freezing protocol of DMSO still remain as the bottleneck of the wide use for clinical applications. Herein, we reported that betaine, a natural zwitterionic molecule, could serve as a nontoxic and high efficient CPA. At optimum concentration of betaine, different cell types exhibited exceptional post-thaw survival efficiency with ultrarapid freezing protocol, which was straightforward, cost efficient but difficult to succeed using DMSO. Moreover, betaine showed negligible cytotoxicity even after long-term exposure of cells. Mechanistically, we hypothesized that betaine could be ultra-rapidly taken up by cells for intracellular protection during the freezing process. This technology unlocks the possibility of alternating the traditional toxic CPAs and is applicable to a variety of clinical applications.
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spelling pubmed-51186952016-11-28 Natural zwitterionic betaine enables cells to survive ultrarapid cryopreservation Yang, Jing Cai, Nana Zhai, Hongwen Zhang, Jiamin Zhu, Yingnan Zhang, Lei Sci Rep Article Cryoprotectants (CPAs) play a critical role in cryopreservation because they can resist the cell damage caused by the freezing process. Current state-of-the-art CPAs are mainly based on an organic solvent dimethyl sulfoxide (DMSO), and several DMSO-cryopreserved cell products have been brought to market. However, the intrinsic toxicity and complex freezing protocol of DMSO still remain as the bottleneck of the wide use for clinical applications. Herein, we reported that betaine, a natural zwitterionic molecule, could serve as a nontoxic and high efficient CPA. At optimum concentration of betaine, different cell types exhibited exceptional post-thaw survival efficiency with ultrarapid freezing protocol, which was straightforward, cost efficient but difficult to succeed using DMSO. Moreover, betaine showed negligible cytotoxicity even after long-term exposure of cells. Mechanistically, we hypothesized that betaine could be ultra-rapidly taken up by cells for intracellular protection during the freezing process. This technology unlocks the possibility of alternating the traditional toxic CPAs and is applicable to a variety of clinical applications. Nature Publishing Group 2016-11-22 /pmc/articles/PMC5118695/ /pubmed/27874036 http://dx.doi.org/10.1038/srep37458 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Yang, Jing
Cai, Nana
Zhai, Hongwen
Zhang, Jiamin
Zhu, Yingnan
Zhang, Lei
Natural zwitterionic betaine enables cells to survive ultrarapid cryopreservation
title Natural zwitterionic betaine enables cells to survive ultrarapid cryopreservation
title_full Natural zwitterionic betaine enables cells to survive ultrarapid cryopreservation
title_fullStr Natural zwitterionic betaine enables cells to survive ultrarapid cryopreservation
title_full_unstemmed Natural zwitterionic betaine enables cells to survive ultrarapid cryopreservation
title_short Natural zwitterionic betaine enables cells to survive ultrarapid cryopreservation
title_sort natural zwitterionic betaine enables cells to survive ultrarapid cryopreservation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5118695/
https://www.ncbi.nlm.nih.gov/pubmed/27874036
http://dx.doi.org/10.1038/srep37458
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