Cargando…

Lipooligosaccharide locus classes and putative virulence genes among chicken and human Campylobacter jejuni isolates

BACKGROUND: Campylobacter cause morbidity and considerable economic loss due to hospitalization and post infectious sequelae such as reactive arthritis, Guillain Barré- and Miller Fischer syndromes. Such sequelae have been linked to C. jejuni harboring sialic acid structures in their lipooligosaccha...

Descripción completa

Detalles Bibliográficos
Autores principales: Ellström, Patrik, Hansson, Ingrid, Nilsson, Anna, Rautelin, Hilpi, Olsson Engvall, Eva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5118878/
https://www.ncbi.nlm.nih.gov/pubmed/27871232
http://dx.doi.org/10.1186/s12866-016-0740-5
_version_ 1782469010489606144
author Ellström, Patrik
Hansson, Ingrid
Nilsson, Anna
Rautelin, Hilpi
Olsson Engvall, Eva
author_facet Ellström, Patrik
Hansson, Ingrid
Nilsson, Anna
Rautelin, Hilpi
Olsson Engvall, Eva
author_sort Ellström, Patrik
collection PubMed
description BACKGROUND: Campylobacter cause morbidity and considerable economic loss due to hospitalization and post infectious sequelae such as reactive arthritis, Guillain Barré- and Miller Fischer syndromes. Such sequelae have been linked to C. jejuni harboring sialic acid structures in their lipooligosaccharide (LOS) layer of the cell wall. Poultry is an important source of human Campylobacter infections but little is known about the prevalence of sialylated C. jejuni isolates and the extent of transmission of such isolates to humans. RESULTS: Genotypes of C. jejuni isolates from enteritis patients were compared with those of broiler chicken with pulsed-field gel electrophoresis (PFGE), to study the patterns of LOS biosynthesis genes and other virulence associated genes and to what extent these occur among Campylobacter genotypes found both in humans and chickens. Chicken and human isolates generally had similar distributions of the putative virulence genes and LOS locus classes studied. However, there were significant differences regarding LOS locus class of PFGE types that were overlapping between chicken and human isolates and those that were distinct to each source. CONCLUSIONS: The study highlights the prevalence of virulence associated genes among Campylobacter isolates from humans and chickens and suggests possible patterns of transmission between the two species.
format Online
Article
Text
id pubmed-5118878
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-51188782016-11-28 Lipooligosaccharide locus classes and putative virulence genes among chicken and human Campylobacter jejuni isolates Ellström, Patrik Hansson, Ingrid Nilsson, Anna Rautelin, Hilpi Olsson Engvall, Eva BMC Microbiol Research Article BACKGROUND: Campylobacter cause morbidity and considerable economic loss due to hospitalization and post infectious sequelae such as reactive arthritis, Guillain Barré- and Miller Fischer syndromes. Such sequelae have been linked to C. jejuni harboring sialic acid structures in their lipooligosaccharide (LOS) layer of the cell wall. Poultry is an important source of human Campylobacter infections but little is known about the prevalence of sialylated C. jejuni isolates and the extent of transmission of such isolates to humans. RESULTS: Genotypes of C. jejuni isolates from enteritis patients were compared with those of broiler chicken with pulsed-field gel electrophoresis (PFGE), to study the patterns of LOS biosynthesis genes and other virulence associated genes and to what extent these occur among Campylobacter genotypes found both in humans and chickens. Chicken and human isolates generally had similar distributions of the putative virulence genes and LOS locus classes studied. However, there were significant differences regarding LOS locus class of PFGE types that were overlapping between chicken and human isolates and those that were distinct to each source. CONCLUSIONS: The study highlights the prevalence of virulence associated genes among Campylobacter isolates from humans and chickens and suggests possible patterns of transmission between the two species. BioMed Central 2016-11-21 /pmc/articles/PMC5118878/ /pubmed/27871232 http://dx.doi.org/10.1186/s12866-016-0740-5 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Ellström, Patrik
Hansson, Ingrid
Nilsson, Anna
Rautelin, Hilpi
Olsson Engvall, Eva
Lipooligosaccharide locus classes and putative virulence genes among chicken and human Campylobacter jejuni isolates
title Lipooligosaccharide locus classes and putative virulence genes among chicken and human Campylobacter jejuni isolates
title_full Lipooligosaccharide locus classes and putative virulence genes among chicken and human Campylobacter jejuni isolates
title_fullStr Lipooligosaccharide locus classes and putative virulence genes among chicken and human Campylobacter jejuni isolates
title_full_unstemmed Lipooligosaccharide locus classes and putative virulence genes among chicken and human Campylobacter jejuni isolates
title_short Lipooligosaccharide locus classes and putative virulence genes among chicken and human Campylobacter jejuni isolates
title_sort lipooligosaccharide locus classes and putative virulence genes among chicken and human campylobacter jejuni isolates
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5118878/
https://www.ncbi.nlm.nih.gov/pubmed/27871232
http://dx.doi.org/10.1186/s12866-016-0740-5
work_keys_str_mv AT ellstrompatrik lipooligosaccharidelocusclassesandputativevirulencegenesamongchickenandhumancampylobacterjejuniisolates
AT hanssoningrid lipooligosaccharidelocusclassesandputativevirulencegenesamongchickenandhumancampylobacterjejuniisolates
AT nilssonanna lipooligosaccharidelocusclassesandputativevirulencegenesamongchickenandhumancampylobacterjejuniisolates
AT rautelinhilpi lipooligosaccharidelocusclassesandputativevirulencegenesamongchickenandhumancampylobacterjejuniisolates
AT olssonengvalleva lipooligosaccharidelocusclassesandputativevirulencegenesamongchickenandhumancampylobacterjejuniisolates