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Accurate Estimation of Fungal Diversity and Abundance through Improved Lineage-Specific Primers Optimized for Illumina Amplicon Sequencing

While high-throughput sequencing methods are revolutionizing fungal ecology, recovering accurate estimates of species richness and abundance has proven elusive. We sought to design internal transcribed spacer (ITS) primers and an Illumina protocol that would maximize coverage of the kingdom Fungi wh...

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Detalles Bibliográficos
Autores principales:	Taylor, D. Lee, Walters, William A., Lennon, Niall J., Bochicchio, James, Krohn, Andrew, Caporaso, J. Gregory, Pennanen, Taina
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society for Microbiology 2016
Materias:	Microbial Ecology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5118932/ https://www.ncbi.nlm.nih.gov/pubmed/27736792 http://dx.doi.org/10.1128/AEM.02576-16

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