Cargando…
Small RNA Library Preparation Method for Next-Generation Sequencing Using Chemical Modifications to Prevent Adapter Dimer Formation
For most sample types, the automation of RNA and DNA sample preparation workflows enables high throughput next-generation sequencing (NGS) library preparation. Greater adoption of small RNA (sRNA) sequencing has been hindered by high sample input requirements and inherent ligation side products form...
Autores principales: | Shore, Sabrina, Henderson, Jordana M., Lebedev, Alexandre, Salcedo, Michelle P., Zon, Gerald, McCaffrey, Anton P., Paul, Natasha, Hogrefe, Richard I. |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5119831/ https://www.ncbi.nlm.nih.gov/pubmed/27875576 http://dx.doi.org/10.1371/journal.pone.0167009 |
Ejemplares similares
-
Uridine Depletion and Chemical Modification Increase Cas9 mRNA Activity and Reduce Immunogenicity without HPLC Purification
por: Vaidyanathan, Sriram, et al.
Publicado: (2018) -
Efficient Production of On-Target Reads for Small
RNA Sequencing of Single Cells Using Modified Adapters
por: Khnouf, Ruba, et al.
Publicado: (2018) -
Hot Start PCR with heat-activatable primers: a novel approach for improved PCR performance
por: Lebedev, Alexandre V., et al.
Publicado: (2008) -
5′ Unlocked Nucleic Acid Modification Improves siRNA Targeting
por: Snead, Nicholas M, et al.
Publicado: (2013) -
Systematic assessment of commercially available low-input miRNA library preparation kits
por: Heinicke, Fatima, et al.
Publicado: (2019)