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Rac1/RhoA antagonism defines cell-to-cell heterogeneity during epidermal morphogenesis in nematodes
The antagonism between the GTPases Rac1 and RhoA controls cell-to-cell heterogeneity in isogenic populations of cells in vitro and epithelial morphogenesis in vivo. Its involvement in the regulation of cell-to-cell heterogeneity during epidermal morphogenesis has, however, never been addressed. We u...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5119937/ https://www.ncbi.nlm.nih.gov/pubmed/27821782 http://dx.doi.org/10.1083/jcb.201604015 |
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author | Martin, Emmanuel Ouellette, Marie-Hélène Jenna, Sarah |
author_facet | Martin, Emmanuel Ouellette, Marie-Hélène Jenna, Sarah |
author_sort | Martin, Emmanuel |
collection | PubMed |
description | The antagonism between the GTPases Rac1 and RhoA controls cell-to-cell heterogeneity in isogenic populations of cells in vitro and epithelial morphogenesis in vivo. Its involvement in the regulation of cell-to-cell heterogeneity during epidermal morphogenesis has, however, never been addressed. We used a quantitative cell imaging approach to characterize epidermal morphogenesis at a single-cell level during early elongation of Caenorhabditis elegans embryos. This study reveals that a Rac1-like pathway, involving the Rac/Cdc42 guanine-exchange factor β-PIX/PIX-1 and effector PAK1/PAK-1, and a RhoA-like pathway, involving ROCK/LET-502, control the remodeling of apical junctions and the formation of basolateral protrusions in distinct subsets of hypodermal cells. In these contexts, protrusions adopt lamellipodia or an amoeboid morphology. We propose that lamella formation may reduce tension building at cell–cell junctions during morphogenesis. Cell-autonomous antagonism between these pathways enables cells to switch between Rac1- and RhoA-like morphogenetic programs. This study identifies the first case of cell-to-cell heterogeneity controlled by Rac1/RhoA antagonism during epidermal morphogenesis. |
format | Online Article Text |
id | pubmed-5119937 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-51199372017-05-21 Rac1/RhoA antagonism defines cell-to-cell heterogeneity during epidermal morphogenesis in nematodes Martin, Emmanuel Ouellette, Marie-Hélène Jenna, Sarah J Cell Biol Research Articles The antagonism between the GTPases Rac1 and RhoA controls cell-to-cell heterogeneity in isogenic populations of cells in vitro and epithelial morphogenesis in vivo. Its involvement in the regulation of cell-to-cell heterogeneity during epidermal morphogenesis has, however, never been addressed. We used a quantitative cell imaging approach to characterize epidermal morphogenesis at a single-cell level during early elongation of Caenorhabditis elegans embryos. This study reveals that a Rac1-like pathway, involving the Rac/Cdc42 guanine-exchange factor β-PIX/PIX-1 and effector PAK1/PAK-1, and a RhoA-like pathway, involving ROCK/LET-502, control the remodeling of apical junctions and the formation of basolateral protrusions in distinct subsets of hypodermal cells. In these contexts, protrusions adopt lamellipodia or an amoeboid morphology. We propose that lamella formation may reduce tension building at cell–cell junctions during morphogenesis. Cell-autonomous antagonism between these pathways enables cells to switch between Rac1- and RhoA-like morphogenetic programs. This study identifies the first case of cell-to-cell heterogeneity controlled by Rac1/RhoA antagonism during epidermal morphogenesis. The Rockefeller University Press 2016-11-21 /pmc/articles/PMC5119937/ /pubmed/27821782 http://dx.doi.org/10.1083/jcb.201604015 Text en © 2016 Martin et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Martin, Emmanuel Ouellette, Marie-Hélène Jenna, Sarah Rac1/RhoA antagonism defines cell-to-cell heterogeneity during epidermal morphogenesis in nematodes |
title | Rac1/RhoA antagonism defines cell-to-cell heterogeneity during epidermal morphogenesis in nematodes |
title_full | Rac1/RhoA antagonism defines cell-to-cell heterogeneity during epidermal morphogenesis in nematodes |
title_fullStr | Rac1/RhoA antagonism defines cell-to-cell heterogeneity during epidermal morphogenesis in nematodes |
title_full_unstemmed | Rac1/RhoA antagonism defines cell-to-cell heterogeneity during epidermal morphogenesis in nematodes |
title_short | Rac1/RhoA antagonism defines cell-to-cell heterogeneity during epidermal morphogenesis in nematodes |
title_sort | rac1/rhoa antagonism defines cell-to-cell heterogeneity during epidermal morphogenesis in nematodes |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5119937/ https://www.ncbi.nlm.nih.gov/pubmed/27821782 http://dx.doi.org/10.1083/jcb.201604015 |
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