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Mapping Quantitative Trait Loci Controlling High Iron and Zinc Content in Self and Open Pollinated Grains of Pearl Millet [Pennisetum glaucum (L.) R. Br.]

Pearl millet is a multipurpose grain/fodder crop of the semi-arid tropics, feeding many of the world’s poorest and most undernourished people. Genetic variation among adapted pearl millet inbreds and hybrids suggests it will be possible to improve grain micronutrient concentrations by selective bree...

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Autores principales: Kumar, Sushil, Hash, Charles T., Thirunavukkarasu, Nepolean, Singh, Govind, Rajaram, Vengaldas, Rathore, Abhishek, Senapathy, Senthilvel, Mahendrakar, Mahesh D., Yadav, Rattan S., Srivastava, Rakesh K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5120122/
https://www.ncbi.nlm.nih.gov/pubmed/27933068
http://dx.doi.org/10.3389/fpls.2016.01636
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author Kumar, Sushil
Hash, Charles T.
Thirunavukkarasu, Nepolean
Singh, Govind
Rajaram, Vengaldas
Rathore, Abhishek
Senapathy, Senthilvel
Mahendrakar, Mahesh D.
Yadav, Rattan S.
Srivastava, Rakesh K.
author_facet Kumar, Sushil
Hash, Charles T.
Thirunavukkarasu, Nepolean
Singh, Govind
Rajaram, Vengaldas
Rathore, Abhishek
Senapathy, Senthilvel
Mahendrakar, Mahesh D.
Yadav, Rattan S.
Srivastava, Rakesh K.
author_sort Kumar, Sushil
collection PubMed
description Pearl millet is a multipurpose grain/fodder crop of the semi-arid tropics, feeding many of the world’s poorest and most undernourished people. Genetic variation among adapted pearl millet inbreds and hybrids suggests it will be possible to improve grain micronutrient concentrations by selective breeding. Using 305 loci, a linkage map was constructed to map QTLs for grain iron [Fe] and zinc [Zn] using replicated samples of 106 pearl millet RILs (F(6)) derived from ICMB 841-P3 × 863B-P2. The grains of the RIL population were evaluated for Fe and Zn content using atomic absorption spectrophotometer. Grain mineral concentrations ranged from 28.4 to 124.0 ppm for Fe and 28.7 to 119.8 ppm for Zn. Similarly, grain Fe and Zn in open pollinated seeds ranged between 22.4–77.4 and 21.9–73.7 ppm, respectively. Mapping with 305 (96 SSRs; 208 DArT) markers detected seven linkage groups covering 1749 cM (Haldane) with an average intermarker distance of 5.73 cM. On the basis of two environment phenotypic data, two co-localized QTLs for Fe and Zn content on linkage group (LG) 3 were identified by composite interval mapping (CIM). Fe QTL explained 19% phenotypic variation, whereas the Zn QTL explained 36% phenotypic variation. Likewise for open pollinated seeds, the QTL analysis led to the identification of two QTLs for grain Fe content on LG3 and 5, and two QTLs for grain Zn content on LG3 and 7. The total phenotypic variance for Fe and Zn QTLs in open pollinated seeds was 16 and 42%, respectively. Analysis of QTL × QTL and QTL × QTL × environment interactions indicated no major epistasis.
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spelling pubmed-51201222016-12-08 Mapping Quantitative Trait Loci Controlling High Iron and Zinc Content in Self and Open Pollinated Grains of Pearl Millet [Pennisetum glaucum (L.) R. Br.] Kumar, Sushil Hash, Charles T. Thirunavukkarasu, Nepolean Singh, Govind Rajaram, Vengaldas Rathore, Abhishek Senapathy, Senthilvel Mahendrakar, Mahesh D. Yadav, Rattan S. Srivastava, Rakesh K. Front Plant Sci Plant Science Pearl millet is a multipurpose grain/fodder crop of the semi-arid tropics, feeding many of the world’s poorest and most undernourished people. Genetic variation among adapted pearl millet inbreds and hybrids suggests it will be possible to improve grain micronutrient concentrations by selective breeding. Using 305 loci, a linkage map was constructed to map QTLs for grain iron [Fe] and zinc [Zn] using replicated samples of 106 pearl millet RILs (F(6)) derived from ICMB 841-P3 × 863B-P2. The grains of the RIL population were evaluated for Fe and Zn content using atomic absorption spectrophotometer. Grain mineral concentrations ranged from 28.4 to 124.0 ppm for Fe and 28.7 to 119.8 ppm for Zn. Similarly, grain Fe and Zn in open pollinated seeds ranged between 22.4–77.4 and 21.9–73.7 ppm, respectively. Mapping with 305 (96 SSRs; 208 DArT) markers detected seven linkage groups covering 1749 cM (Haldane) with an average intermarker distance of 5.73 cM. On the basis of two environment phenotypic data, two co-localized QTLs for Fe and Zn content on linkage group (LG) 3 were identified by composite interval mapping (CIM). Fe QTL explained 19% phenotypic variation, whereas the Zn QTL explained 36% phenotypic variation. Likewise for open pollinated seeds, the QTL analysis led to the identification of two QTLs for grain Fe content on LG3 and 5, and two QTLs for grain Zn content on LG3 and 7. The total phenotypic variance for Fe and Zn QTLs in open pollinated seeds was 16 and 42%, respectively. Analysis of QTL × QTL and QTL × QTL × environment interactions indicated no major epistasis. Frontiers Media S.A. 2016-11-23 /pmc/articles/PMC5120122/ /pubmed/27933068 http://dx.doi.org/10.3389/fpls.2016.01636 Text en Copyright © 2016 Kumar, Hash, Thirunavukkarasu, Singh, Rajaram, Rathore, Senapathy, Mahendrakar, Yadav and Srivastava. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Kumar, Sushil
Hash, Charles T.
Thirunavukkarasu, Nepolean
Singh, Govind
Rajaram, Vengaldas
Rathore, Abhishek
Senapathy, Senthilvel
Mahendrakar, Mahesh D.
Yadav, Rattan S.
Srivastava, Rakesh K.
Mapping Quantitative Trait Loci Controlling High Iron and Zinc Content in Self and Open Pollinated Grains of Pearl Millet [Pennisetum glaucum (L.) R. Br.]
title Mapping Quantitative Trait Loci Controlling High Iron and Zinc Content in Self and Open Pollinated Grains of Pearl Millet [Pennisetum glaucum (L.) R. Br.]
title_full Mapping Quantitative Trait Loci Controlling High Iron and Zinc Content in Self and Open Pollinated Grains of Pearl Millet [Pennisetum glaucum (L.) R. Br.]
title_fullStr Mapping Quantitative Trait Loci Controlling High Iron and Zinc Content in Self and Open Pollinated Grains of Pearl Millet [Pennisetum glaucum (L.) R. Br.]
title_full_unstemmed Mapping Quantitative Trait Loci Controlling High Iron and Zinc Content in Self and Open Pollinated Grains of Pearl Millet [Pennisetum glaucum (L.) R. Br.]
title_short Mapping Quantitative Trait Loci Controlling High Iron and Zinc Content in Self and Open Pollinated Grains of Pearl Millet [Pennisetum glaucum (L.) R. Br.]
title_sort mapping quantitative trait loci controlling high iron and zinc content in self and open pollinated grains of pearl millet [pennisetum glaucum (l.) r. br.]
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5120122/
https://www.ncbi.nlm.nih.gov/pubmed/27933068
http://dx.doi.org/10.3389/fpls.2016.01636
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