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Determination of Genotoxic Effects of Hookah Smoking by Micronucleus and Chromosome Aberration Methods
BACKGROUND: Use of a hookah (a type of water pipe) is a traditional way of smoking tobacco, particularly in the Middle East. In Turkey, its popularity has been growing in recent years, especially among young people. It is known that cigarette smoking has genotoxic effects and causes mutations, but n...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Scientific Literature, Inc.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5120646/ https://www.ncbi.nlm.nih.gov/pubmed/27869111 http://dx.doi.org/10.12659/MSM.898593 |
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author | Eker, Ebru Derici Koyuncu, Hayri Şahin, Nefise Özlen Yüksel, Altan Berköz, Mehmet Diler, Songul Budak Akgül, Sema Altan |
author_facet | Eker, Ebru Derici Koyuncu, Hayri Şahin, Nefise Özlen Yüksel, Altan Berköz, Mehmet Diler, Songul Budak Akgül, Sema Altan |
author_sort | Eker, Ebru Derici |
collection | PubMed |
description | BACKGROUND: Use of a hookah (a type of water pipe) is a traditional way of smoking tobacco, particularly in the Middle East. In Turkey, its popularity has been growing in recent years, especially among young people. It is known that cigarette smoking has genotoxic effects and causes mutations, but no comprehensive study has been done on the genotoxic effects of hookah usage, particularly in Turkey. MATERIAL/METHODS: We collected peripheral blood/buccal smear samples from 30 subjects who did not smoke cigarettes but who regularly smoke a hookah an average of 2 times per week, and from 30 control subjects who had never smoked cigarettes or a hookah. Chromosome analyses were performed on the samples obtained from peripheral blood of each individual, 25 metaphase plaques were counted for each, and chromosome/chromatid breakage/gap parameters were evaluated. Micronucleus analysis was done on buccal smear samples and micronucleus/binucleus parameters were investigated by counting 2000 cells of each individual. RESULTS: Chromosome breakage ratios were found to be 0.64±0.86 and 0.46±0.71 in the study and control groups, respectively, while chromatid breakage ratios were 0.53±0.83 and 0.53±0.71; fragment ratios were 0.82±1.24 and 0.21±0.49 (p<0.05); and gap ratios were 0.57±0.83 and 0.18±0.53 (p<0.05), respectively. Micronucleus ratio was 6.03±2.06 and 4.43±2.27 (p<0.05) in the study and control groups, respectively, and binucleus ratios were 8.53±3.23 and 12.15±5.18, respectively (p<0.05). CONCLUSIONS: Results of our study reveal significant statistical differences between the individuals who smoked hookah and those who did not in terms of fragment, gap, micronucleus, and binucleus parameters, suggesting that smoking a hookah may cause genotoxic effects. |
format | Online Article Text |
id | pubmed-5120646 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | International Scientific Literature, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-51206462016-11-29 Determination of Genotoxic Effects of Hookah Smoking by Micronucleus and Chromosome Aberration Methods Eker, Ebru Derici Koyuncu, Hayri Şahin, Nefise Özlen Yüksel, Altan Berköz, Mehmet Diler, Songul Budak Akgül, Sema Altan Med Sci Monit Clinical Research BACKGROUND: Use of a hookah (a type of water pipe) is a traditional way of smoking tobacco, particularly in the Middle East. In Turkey, its popularity has been growing in recent years, especially among young people. It is known that cigarette smoking has genotoxic effects and causes mutations, but no comprehensive study has been done on the genotoxic effects of hookah usage, particularly in Turkey. MATERIAL/METHODS: We collected peripheral blood/buccal smear samples from 30 subjects who did not smoke cigarettes but who regularly smoke a hookah an average of 2 times per week, and from 30 control subjects who had never smoked cigarettes or a hookah. Chromosome analyses were performed on the samples obtained from peripheral blood of each individual, 25 metaphase plaques were counted for each, and chromosome/chromatid breakage/gap parameters were evaluated. Micronucleus analysis was done on buccal smear samples and micronucleus/binucleus parameters were investigated by counting 2000 cells of each individual. RESULTS: Chromosome breakage ratios were found to be 0.64±0.86 and 0.46±0.71 in the study and control groups, respectively, while chromatid breakage ratios were 0.53±0.83 and 0.53±0.71; fragment ratios were 0.82±1.24 and 0.21±0.49 (p<0.05); and gap ratios were 0.57±0.83 and 0.18±0.53 (p<0.05), respectively. Micronucleus ratio was 6.03±2.06 and 4.43±2.27 (p<0.05) in the study and control groups, respectively, and binucleus ratios were 8.53±3.23 and 12.15±5.18, respectively (p<0.05). CONCLUSIONS: Results of our study reveal significant statistical differences between the individuals who smoked hookah and those who did not in terms of fragment, gap, micronucleus, and binucleus parameters, suggesting that smoking a hookah may cause genotoxic effects. International Scientific Literature, Inc. 2016-11-21 /pmc/articles/PMC5120646/ /pubmed/27869111 http://dx.doi.org/10.12659/MSM.898593 Text en © Med Sci Monit, 2016 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) |
spellingShingle | Clinical Research Eker, Ebru Derici Koyuncu, Hayri Şahin, Nefise Özlen Yüksel, Altan Berköz, Mehmet Diler, Songul Budak Akgül, Sema Altan Determination of Genotoxic Effects of Hookah Smoking by Micronucleus and Chromosome Aberration Methods |
title | Determination of Genotoxic Effects of Hookah Smoking by Micronucleus and Chromosome Aberration Methods |
title_full | Determination of Genotoxic Effects of Hookah Smoking by Micronucleus and Chromosome Aberration Methods |
title_fullStr | Determination of Genotoxic Effects of Hookah Smoking by Micronucleus and Chromosome Aberration Methods |
title_full_unstemmed | Determination of Genotoxic Effects of Hookah Smoking by Micronucleus and Chromosome Aberration Methods |
title_short | Determination of Genotoxic Effects of Hookah Smoking by Micronucleus and Chromosome Aberration Methods |
title_sort | determination of genotoxic effects of hookah smoking by micronucleus and chromosome aberration methods |
topic | Clinical Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5120646/ https://www.ncbi.nlm.nih.gov/pubmed/27869111 http://dx.doi.org/10.12659/MSM.898593 |
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