Making standards for quantitative real-time pneumococcal PCR

Quantitative lytA PCR is often performed using in-house standards. We hypothesised equivalence when measuring a standard suspension of Streptococcus pneumoniae by colony-forming-units (CFU) or genome-copies. Median (IQR) ratio of CFU/genome-copies was 0.19 (0.1–1.2). Genome-copies were less variable...

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Detalles Bibliográficos
Autores principales: Morpeth, Susan C., Huggett, Jim F., Murdoch, David R., Scott, J. Anthony G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5121202/
https://www.ncbi.nlm.nih.gov/pubmed/27896138
http://dx.doi.org/10.1016/j.bdq.2014.11.003
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author Morpeth, Susan C.
Huggett, Jim F.
Murdoch, David R.
Scott, J. Anthony G.
author_facet Morpeth, Susan C.
Huggett, Jim F.
Murdoch, David R.
Scott, J. Anthony G.
author_sort Morpeth, Susan C.
collection PubMed
description Quantitative lytA PCR is often performed using in-house standards. We hypothesised equivalence when measuring a standard suspension of Streptococcus pneumoniae by colony-forming-units (CFU) or genome-copies. Median (IQR) ratio of CFU/genome-copies was 0.19 (0.1–1.2). Genome-copies were less variable than CFU, but the discrepancy between the methods highlights challenges with absolute quantification.
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spelling pubmed-51212022016-11-28 Making standards for quantitative real-time pneumococcal PCR Morpeth, Susan C. Huggett, Jim F. Murdoch, David R. Scott, J. Anthony G. Biomol Detect Quantif Short Communication Quantitative lytA PCR is often performed using in-house standards. We hypothesised equivalence when measuring a standard suspension of Streptococcus pneumoniae by colony-forming-units (CFU) or genome-copies. Median (IQR) ratio of CFU/genome-copies was 0.19 (0.1–1.2). Genome-copies were less variable than CFU, but the discrepancy between the methods highlights challenges with absolute quantification. Elsevier 2014-12-04 /pmc/articles/PMC5121202/ /pubmed/27896138 http://dx.doi.org/10.1016/j.bdq.2014.11.003 Text en © 2014 The Authors http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
spellingShingle Short Communication
Morpeth, Susan C.
Huggett, Jim F.
Murdoch, David R.
Scott, J. Anthony G.
Making standards for quantitative real-time pneumococcal PCR
title Making standards for quantitative real-time pneumococcal PCR
title_full Making standards for quantitative real-time pneumococcal PCR
title_fullStr Making standards for quantitative real-time pneumococcal PCR
title_full_unstemmed Making standards for quantitative real-time pneumococcal PCR
title_short Making standards for quantitative real-time pneumococcal PCR
title_sort making standards for quantitative real-time pneumococcal pcr
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5121202/
https://www.ncbi.nlm.nih.gov/pubmed/27896138
http://dx.doi.org/10.1016/j.bdq.2014.11.003
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