Cargando…

Making standards for quantitative real-time pneumococcal PCR

Quantitative lytA PCR is often performed using in-house standards. We hypothesised equivalence when measuring a standard suspension of Streptococcus pneumoniae by colony-forming-units (CFU) or genome-copies. Median (IQR) ratio of CFU/genome-copies was 0.19 (0.1–1.2). Genome-copies were less variable...

Descripción completa

Detalles Bibliográficos
Autores principales:	Morpeth, Susan C., Huggett, Jim F., Murdoch, David R., Scott, J. Anthony G.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2014
Materias:	Short Communication
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5121202/ https://www.ncbi.nlm.nih.gov/pubmed/27896138 http://dx.doi.org/10.1016/j.bdq.2014.11.003

Ejemplares similares

Improving the standardization of mRNA measurement by RT-qPCR
por: Sanders, Rebecca, et al.
Publicado: (2018)

Comparison of droplet digital PCR and quantitative real-time PCR in mcrA-based methanogen community analysis
por: Kim, Tae Gwan, et al.
Publicado: (2014)

MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments
por: Bustin, Stephen A, et al.
Publicado: (2010)

Clinical performance of a quantitative pan-genus Leishmania Real-time PCR assay for diagnosis of cutaneous and visceral leishmaniasis
por: Ramírez, Juan David, et al.
Publicado: (2023)

Whatman FTA cards versus plasma specimens for the quantitation of HIV-1 RNA using two real-time PCR assays
por: Yacouba, Abdourahamane, et al.
Publicado: (2020)

Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD
por: Leal, C.A.G., et al.
Publicado: (2013)

Development of a SYBR Green based real-time PCR assay for detection and quantitation of canine parvovirus in faecal samples
por: Kumar, Manoj, et al.
Publicado: (2010)

Quantification of bacteria by in vivo bioluminescence imaging in comparison with standard spread plate method and reverse transcription quantitative PCR (RT-qPCR)
por: Briestenská, Katarína, et al.
Publicado: (2021)

An updated TaqMan real-time PCR for canine and feline parvoviruses
por: Streck, André Felipe, et al.
Publicado: (2013)

Comparison of real-time PCR and hemagglutination assay for quantitation of human polyomavirus JC
por: Chapagain, Moti L, et al.
Publicado: (2006)

Development of a real-time SYBR Green PCR assay for the rapid detection of Dermatophilus congolensis
por: García, Alfredo, et al.
Publicado: (2013)

Real-time PCR assay for the detection of tanapox virus and yaba-like disease virus
por: Zimmermann, Pia, et al.
Publicado: (2005)

Development of a Taqman Real-Time PCR for the Identification of Haemaphysalis longicornis (Acari: Ixodidae)
por: Xu, Guang, et al.
Publicado: (2022)

Designing Two Synthetic Constructs for Real Time PCR Detection of Francisella tularensis and Ebola Virus
por: Dehghan Esmat Abadi, Mohammadjavad, et al.
Publicado: (2021)

Analysis of ZAP70 expression in adult acute lymphoblastic leukaemia by real time quantitative PCR
por: Chakupurakal, Geothy, et al.
Publicado: (2012)

Development and use of real-time PCR to detect and quantify Mycoplasma haemocanis and “Candidatus Mycoplasma haematoparvum” in dogs
por: Barker, E.N., et al.
Publicado: (2010)

Development of an SYBR Green I-based real-time PCR assay for the rapid detection of canine kobuvirus
por: Wang, Yong, et al.
Publicado: (2020)

Comparative evaluation of Panther Fusion and real-time PCR for detection of Burkholderia pseudomallei in spiked human blood
por: Gassiep, Ian, et al.
Publicado: (2022)

Development of real-time RT-PCR assays to detect measles virus on the Hologic Panther Fusion® System
por: Grant, Jenny, et al.
Publicado: (2023)

Quantitative Real-Time PCR
por: Sluijter, J. P. G., et al.
Publicado: (2006)

Implementation of an in-house real-time reverse transcription-PCR assay for the rapid detection of the SARS-CoV-2 Marseille-4 variant
por: Bedotto, Marielle, et al.
Publicado: (2021)

Detection of Capripoxvirus DNA Using a Field‐Ready Nucleic Acid Extraction and Real‐Time PCR Platform
por: Armson, B., et al.
Publicado: (2015)

Real-time PCR assays for the specific detection of monkeypox virus West African and Congo Basin strain DNA
por: Li, Yu, et al.
Publicado: (2010)

Lack of correlation between in silico projection of function and quantitative real-time PCR-determined gene expression levels in colon tissue
por: Penney, Rosalind B, et al.
Publicado: (2013)

Comparison of culture, single and multiplex real‐time PCR for detection of Sabin poliovirus shedding in recently vaccinated Indian children
por: Giri, Sidhartha, et al.
Publicado: (2017)

Application of real-time PCR for testing antiviral compounds against Lassa virus, SARS coronavirus and Ebola virus in vitro
por: Günther, Stephan, et al.
Publicado: (2004)

Performance of SARS-CoV-2 rapid antigen test compared with real-time RT-PCR in asymptomatic individuals
por: Peña, Mónica, et al.
Publicado: (2021)

How to choose the right real-time RT-PCR primer sets for the SARS-CoV-2 genome detection?
por: Anantharajah, Ahalieyah, et al.
Publicado: (2021)

Prevalence of COVID-19 in 10,000 samples from 7853 patients in Eastern Turkey by positive real-time PCR
por: Karamese, Murat, et al.
Publicado: (2021)

The emerging role of real-world data in advanced breast cancer therapy: Recommendations for collaborative decision-making
por: Cottu, Paul, et al.
Publicado: (2021)

Impact of viral upper respiratory tract infection on the concentration of nasopharyngeal pneumococcal carriage among Kenyan children
por: Morpeth, Susan C., et al.
Publicado: (2018)

Ability of quantitative PCR to discriminate Pneumocystis jirovecii pneumonia from colonization
por: Perret, Thomas, et al.
Publicado: (2020)

Selection of reference genes for normalization of quantitative real-time PCR in organ culture of the rat and rabbit intervertebral disc
por: Seol, Dongrim, et al.
Publicado: (2011)

Enumeration of Mycobacterium avium subsp. paratuberculosis by quantitative real-time PCR, culture on solid media and optical densitometry
por: Kralik, Petr, et al.
Publicado: (2012)

Selection of reference genes for quantitative real-time PCR in equine in vivo and fresh and frozen-thawed in vitro blastocysts
por: Smits, Katrien, et al.
Publicado: (2009)

The First Investigation of West Nile Virus in Horses Using Real Time RT-PCR in Middle Black Sea Region in Turkey
por: Yazici, Zafer, et al.
Publicado: (2012)

Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation
por: Whale, Alexandra S., et al.
Publicado: (2012)

Design and Application of Nucleic Acid Standards for Quantitative Detection of Enteric Viruses by Real-Time PCR
por: Martínez-Martínez, Mónica, et al.
Publicado: (2011)

Quantitation of pulmonary fungal burden in Paracoccidioides brasiliensis-infected mice by real-time PCR
por: Costa, Marcelo Vieira, et al.
Publicado: (2018)

Genomic DNA functions as a universal external standard in quantitative real-time PCR
por: Yun, James J., et al.
Publicado: (2006)

Cannot write session to /tmp/vufind_sessions/sess_ntk5bq8gghmsskutqdana4riup