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A single heterochronic blood exchange reveals rapid inhibition of multiple tissues by old blood
Heterochronic parabiosis rejuvenates the performance of old tissue stem cells at some expense to the young, but whether this is through shared circulation or shared organs is unclear. Here we show that heterochronic blood exchange between young and old mice without sharing other organs, affects tiss...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5121415/ https://www.ncbi.nlm.nih.gov/pubmed/27874859 http://dx.doi.org/10.1038/ncomms13363 |
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author | Rebo, Justin Mehdipour, Melod Gathwala, Ranveer Causey, Keith Liu, Yan Conboy, Michael J. Conboy, Irina M. |
author_facet | Rebo, Justin Mehdipour, Melod Gathwala, Ranveer Causey, Keith Liu, Yan Conboy, Michael J. Conboy, Irina M. |
author_sort | Rebo, Justin |
collection | PubMed |
description | Heterochronic parabiosis rejuvenates the performance of old tissue stem cells at some expense to the young, but whether this is through shared circulation or shared organs is unclear. Here we show that heterochronic blood exchange between young and old mice without sharing other organs, affects tissues within a few days, and leads to different outcomes than heterochronic parabiosis. Investigating muscle, liver and brain hippocampus, in the presence or absence of muscle injury, we find that, in many cases, the inhibitory effects of old blood are more pronounced than the benefits of young, and that peripheral tissue injury compounds the negative effects. We also explore mechanistic explanations, including the role of B2M and TGF-beta. We conclude that, compared with heterochronic parabiosis, heterochronic blood exchange in small animals is less invasive and enables better-controlled studies with more immediate translation to therapies for humans. |
format | Online Article Text |
id | pubmed-5121415 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-51214152016-12-02 A single heterochronic blood exchange reveals rapid inhibition of multiple tissues by old blood Rebo, Justin Mehdipour, Melod Gathwala, Ranveer Causey, Keith Liu, Yan Conboy, Michael J. Conboy, Irina M. Nat Commun Article Heterochronic parabiosis rejuvenates the performance of old tissue stem cells at some expense to the young, but whether this is through shared circulation or shared organs is unclear. Here we show that heterochronic blood exchange between young and old mice without sharing other organs, affects tissues within a few days, and leads to different outcomes than heterochronic parabiosis. Investigating muscle, liver and brain hippocampus, in the presence or absence of muscle injury, we find that, in many cases, the inhibitory effects of old blood are more pronounced than the benefits of young, and that peripheral tissue injury compounds the negative effects. We also explore mechanistic explanations, including the role of B2M and TGF-beta. We conclude that, compared with heterochronic parabiosis, heterochronic blood exchange in small animals is less invasive and enables better-controlled studies with more immediate translation to therapies for humans. Nature Publishing Group 2016-11-22 /pmc/articles/PMC5121415/ /pubmed/27874859 http://dx.doi.org/10.1038/ncomms13363 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Rebo, Justin Mehdipour, Melod Gathwala, Ranveer Causey, Keith Liu, Yan Conboy, Michael J. Conboy, Irina M. A single heterochronic blood exchange reveals rapid inhibition of multiple tissues by old blood |
title | A single heterochronic blood exchange reveals rapid inhibition of multiple tissues by old blood |
title_full | A single heterochronic blood exchange reveals rapid inhibition of multiple tissues by old blood |
title_fullStr | A single heterochronic blood exchange reveals rapid inhibition of multiple tissues by old blood |
title_full_unstemmed | A single heterochronic blood exchange reveals rapid inhibition of multiple tissues by old blood |
title_short | A single heterochronic blood exchange reveals rapid inhibition of multiple tissues by old blood |
title_sort | single heterochronic blood exchange reveals rapid inhibition of multiple tissues by old blood |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5121415/ https://www.ncbi.nlm.nih.gov/pubmed/27874859 http://dx.doi.org/10.1038/ncomms13363 |
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