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A comparison of the performance of molecularly imprinted polymer nanoparticles for small molecule targets and antibodies in the ELISA format

Here we show that molecularly imprinted polymer nanoparticles, prepared in aqueous media by solid phase synthesis with immobilised L-thyroxine, glucosamine, fumonisin B2 or biotin as template, can demonstrate comparable or better performance to commercially produced antibodies in enzyme-linked compe...

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Autores principales: Smolinska-Kempisty, Katarzyna, Guerreiro, Antonio, Canfarotta, Francesco, Cáceres, César, Whitcombe, Michael J., Piletsky, Sergey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5121619/
https://www.ncbi.nlm.nih.gov/pubmed/27883023
http://dx.doi.org/10.1038/srep37638
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author Smolinska-Kempisty, Katarzyna
Guerreiro, Antonio
Canfarotta, Francesco
Cáceres, César
Whitcombe, Michael J.
Piletsky, Sergey
author_facet Smolinska-Kempisty, Katarzyna
Guerreiro, Antonio
Canfarotta, Francesco
Cáceres, César
Whitcombe, Michael J.
Piletsky, Sergey
author_sort Smolinska-Kempisty, Katarzyna
collection PubMed
description Here we show that molecularly imprinted polymer nanoparticles, prepared in aqueous media by solid phase synthesis with immobilised L-thyroxine, glucosamine, fumonisin B2 or biotin as template, can demonstrate comparable or better performance to commercially produced antibodies in enzyme-linked competitive assays. Imprinted nanoparticles-based assays showed detection limits in the pM range and polymer-coated microplates are stable to storage at room temperature for at least 1 month. No response to analyte was detected in control experiments with nanoparticles imprinted with an unrelated template (trypsin) but prepared with the same polymer composition. The ease of preparation, high affinity of solid-phase synthesised imprinted nanoparticles and the lack of requirement for cold chain logistics make them an attractive alternative to traditional antibodies for use in immunoassays.
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spelling pubmed-51216192016-11-28 A comparison of the performance of molecularly imprinted polymer nanoparticles for small molecule targets and antibodies in the ELISA format Smolinska-Kempisty, Katarzyna Guerreiro, Antonio Canfarotta, Francesco Cáceres, César Whitcombe, Michael J. Piletsky, Sergey Sci Rep Article Here we show that molecularly imprinted polymer nanoparticles, prepared in aqueous media by solid phase synthesis with immobilised L-thyroxine, glucosamine, fumonisin B2 or biotin as template, can demonstrate comparable or better performance to commercially produced antibodies in enzyme-linked competitive assays. Imprinted nanoparticles-based assays showed detection limits in the pM range and polymer-coated microplates are stable to storage at room temperature for at least 1 month. No response to analyte was detected in control experiments with nanoparticles imprinted with an unrelated template (trypsin) but prepared with the same polymer composition. The ease of preparation, high affinity of solid-phase synthesised imprinted nanoparticles and the lack of requirement for cold chain logistics make them an attractive alternative to traditional antibodies for use in immunoassays. Nature Publishing Group 2016-11-24 /pmc/articles/PMC5121619/ /pubmed/27883023 http://dx.doi.org/10.1038/srep37638 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Smolinska-Kempisty, Katarzyna
Guerreiro, Antonio
Canfarotta, Francesco
Cáceres, César
Whitcombe, Michael J.
Piletsky, Sergey
A comparison of the performance of molecularly imprinted polymer nanoparticles for small molecule targets and antibodies in the ELISA format
title A comparison of the performance of molecularly imprinted polymer nanoparticles for small molecule targets and antibodies in the ELISA format
title_full A comparison of the performance of molecularly imprinted polymer nanoparticles for small molecule targets and antibodies in the ELISA format
title_fullStr A comparison of the performance of molecularly imprinted polymer nanoparticles for small molecule targets and antibodies in the ELISA format
title_full_unstemmed A comparison of the performance of molecularly imprinted polymer nanoparticles for small molecule targets and antibodies in the ELISA format
title_short A comparison of the performance of molecularly imprinted polymer nanoparticles for small molecule targets and antibodies in the ELISA format
title_sort comparison of the performance of molecularly imprinted polymer nanoparticles for small molecule targets and antibodies in the elisa format
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5121619/
https://www.ncbi.nlm.nih.gov/pubmed/27883023
http://dx.doi.org/10.1038/srep37638
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