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A comparison of the performance of molecularly imprinted polymer nanoparticles for small molecule targets and antibodies in the ELISA format
Here we show that molecularly imprinted polymer nanoparticles, prepared in aqueous media by solid phase synthesis with immobilised L-thyroxine, glucosamine, fumonisin B2 or biotin as template, can demonstrate comparable or better performance to commercially produced antibodies in enzyme-linked compe...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5121619/ https://www.ncbi.nlm.nih.gov/pubmed/27883023 http://dx.doi.org/10.1038/srep37638 |
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author | Smolinska-Kempisty, Katarzyna Guerreiro, Antonio Canfarotta, Francesco Cáceres, César Whitcombe, Michael J. Piletsky, Sergey |
author_facet | Smolinska-Kempisty, Katarzyna Guerreiro, Antonio Canfarotta, Francesco Cáceres, César Whitcombe, Michael J. Piletsky, Sergey |
author_sort | Smolinska-Kempisty, Katarzyna |
collection | PubMed |
description | Here we show that molecularly imprinted polymer nanoparticles, prepared in aqueous media by solid phase synthesis with immobilised L-thyroxine, glucosamine, fumonisin B2 or biotin as template, can demonstrate comparable or better performance to commercially produced antibodies in enzyme-linked competitive assays. Imprinted nanoparticles-based assays showed detection limits in the pM range and polymer-coated microplates are stable to storage at room temperature for at least 1 month. No response to analyte was detected in control experiments with nanoparticles imprinted with an unrelated template (trypsin) but prepared with the same polymer composition. The ease of preparation, high affinity of solid-phase synthesised imprinted nanoparticles and the lack of requirement for cold chain logistics make them an attractive alternative to traditional antibodies for use in immunoassays. |
format | Online Article Text |
id | pubmed-5121619 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-51216192016-11-28 A comparison of the performance of molecularly imprinted polymer nanoparticles for small molecule targets and antibodies in the ELISA format Smolinska-Kempisty, Katarzyna Guerreiro, Antonio Canfarotta, Francesco Cáceres, César Whitcombe, Michael J. Piletsky, Sergey Sci Rep Article Here we show that molecularly imprinted polymer nanoparticles, prepared in aqueous media by solid phase synthesis with immobilised L-thyroxine, glucosamine, fumonisin B2 or biotin as template, can demonstrate comparable or better performance to commercially produced antibodies in enzyme-linked competitive assays. Imprinted nanoparticles-based assays showed detection limits in the pM range and polymer-coated microplates are stable to storage at room temperature for at least 1 month. No response to analyte was detected in control experiments with nanoparticles imprinted with an unrelated template (trypsin) but prepared with the same polymer composition. The ease of preparation, high affinity of solid-phase synthesised imprinted nanoparticles and the lack of requirement for cold chain logistics make them an attractive alternative to traditional antibodies for use in immunoassays. Nature Publishing Group 2016-11-24 /pmc/articles/PMC5121619/ /pubmed/27883023 http://dx.doi.org/10.1038/srep37638 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Smolinska-Kempisty, Katarzyna Guerreiro, Antonio Canfarotta, Francesco Cáceres, César Whitcombe, Michael J. Piletsky, Sergey A comparison of the performance of molecularly imprinted polymer nanoparticles for small molecule targets and antibodies in the ELISA format |
title | A comparison of the performance of molecularly imprinted polymer nanoparticles for small molecule targets and antibodies in the ELISA format |
title_full | A comparison of the performance of molecularly imprinted polymer nanoparticles for small molecule targets and antibodies in the ELISA format |
title_fullStr | A comparison of the performance of molecularly imprinted polymer nanoparticles for small molecule targets and antibodies in the ELISA format |
title_full_unstemmed | A comparison of the performance of molecularly imprinted polymer nanoparticles for small molecule targets and antibodies in the ELISA format |
title_short | A comparison of the performance of molecularly imprinted polymer nanoparticles for small molecule targets and antibodies in the ELISA format |
title_sort | comparison of the performance of molecularly imprinted polymer nanoparticles for small molecule targets and antibodies in the elisa format |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5121619/ https://www.ncbi.nlm.nih.gov/pubmed/27883023 http://dx.doi.org/10.1038/srep37638 |
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