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Proteomic profile of the Bradysia odoriphaga in response to the microbial secondary metabolite benzothiazole
Benzothiazole, a microbial secondary metabolite, has been demonstrated to possess fumigant activity against Sclerotinia sclerotiorum, Ditylenchus destructor and Bradysia odoriphaga. However, to facilitate the development of novel microbial pesticides, the mode of action of benzothiazole needs to be...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5121901/ https://www.ncbi.nlm.nih.gov/pubmed/27883048 http://dx.doi.org/10.1038/srep37730 |
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author | Zhao, Yunhe Cui, Kaidi Xu, Chunmei Wang, Qiuhong Wang, Yao Zhang, Zhengqun Liu, Feng Mu, Wei |
author_facet | Zhao, Yunhe Cui, Kaidi Xu, Chunmei Wang, Qiuhong Wang, Yao Zhang, Zhengqun Liu, Feng Mu, Wei |
author_sort | Zhao, Yunhe |
collection | PubMed |
description | Benzothiazole, a microbial secondary metabolite, has been demonstrated to possess fumigant activity against Sclerotinia sclerotiorum, Ditylenchus destructor and Bradysia odoriphaga. However, to facilitate the development of novel microbial pesticides, the mode of action of benzothiazole needs to be elucidated. Here, we employed iTRAQ-based quantitative proteomics analysis to investigate the effects of benzothiazole on the proteomic expression of B. odoriphaga. In response to benzothiazole, 92 of 863 identified proteins in B. odoriphaga exhibited altered levels of expression, among which 14 proteins were related to the action mechanism of benzothiazole, 11 proteins were involved in stress responses, and 67 proteins were associated with the adaptation of B. odoriphaga to benzothiazole. Further bioinformatics analysis indicated that the reduction in energy metabolism, inhibition of the detoxification process and interference with DNA and RNA synthesis were potentially associated with the mode of action of benzothiazole. The myosin heavy chain, succinyl-CoA synthetase and Ca(+)-transporting ATPase proteins may be related to the stress response. Increased expression of proteins involved in carbohydrate metabolism, energy production and conversion pathways was responsible for the adaptive response of B. odoriphaga. The results of this study provide novel insight into the molecular mechanisms of benzothiazole at a large-scale translation level and will facilitate the elucidation of the mechanism of action of benzothiazole. |
format | Online Article Text |
id | pubmed-5121901 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-51219012016-11-28 Proteomic profile of the Bradysia odoriphaga in response to the microbial secondary metabolite benzothiazole Zhao, Yunhe Cui, Kaidi Xu, Chunmei Wang, Qiuhong Wang, Yao Zhang, Zhengqun Liu, Feng Mu, Wei Sci Rep Article Benzothiazole, a microbial secondary metabolite, has been demonstrated to possess fumigant activity against Sclerotinia sclerotiorum, Ditylenchus destructor and Bradysia odoriphaga. However, to facilitate the development of novel microbial pesticides, the mode of action of benzothiazole needs to be elucidated. Here, we employed iTRAQ-based quantitative proteomics analysis to investigate the effects of benzothiazole on the proteomic expression of B. odoriphaga. In response to benzothiazole, 92 of 863 identified proteins in B. odoriphaga exhibited altered levels of expression, among which 14 proteins were related to the action mechanism of benzothiazole, 11 proteins were involved in stress responses, and 67 proteins were associated with the adaptation of B. odoriphaga to benzothiazole. Further bioinformatics analysis indicated that the reduction in energy metabolism, inhibition of the detoxification process and interference with DNA and RNA synthesis were potentially associated with the mode of action of benzothiazole. The myosin heavy chain, succinyl-CoA synthetase and Ca(+)-transporting ATPase proteins may be related to the stress response. Increased expression of proteins involved in carbohydrate metabolism, energy production and conversion pathways was responsible for the adaptive response of B. odoriphaga. The results of this study provide novel insight into the molecular mechanisms of benzothiazole at a large-scale translation level and will facilitate the elucidation of the mechanism of action of benzothiazole. Nature Publishing Group 2016-11-24 /pmc/articles/PMC5121901/ /pubmed/27883048 http://dx.doi.org/10.1038/srep37730 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Zhao, Yunhe Cui, Kaidi Xu, Chunmei Wang, Qiuhong Wang, Yao Zhang, Zhengqun Liu, Feng Mu, Wei Proteomic profile of the Bradysia odoriphaga in response to the microbial secondary metabolite benzothiazole |
title | Proteomic profile of the Bradysia odoriphaga in response to the microbial secondary metabolite benzothiazole |
title_full | Proteomic profile of the Bradysia odoriphaga in response to the microbial secondary metabolite benzothiazole |
title_fullStr | Proteomic profile of the Bradysia odoriphaga in response to the microbial secondary metabolite benzothiazole |
title_full_unstemmed | Proteomic profile of the Bradysia odoriphaga in response to the microbial secondary metabolite benzothiazole |
title_short | Proteomic profile of the Bradysia odoriphaga in response to the microbial secondary metabolite benzothiazole |
title_sort | proteomic profile of the bradysia odoriphaga in response to the microbial secondary metabolite benzothiazole |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5121901/ https://www.ncbi.nlm.nih.gov/pubmed/27883048 http://dx.doi.org/10.1038/srep37730 |
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