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Klotho sensitive regulation of dendritic cell functions by vitamin E

BACKGROUND: Dendritic cells (DCs) are the most potent professional antigen-presenting cells for naive T cells to link innate and acquired immunity. Klotho, an anti-aging protein, participates in the regulation of Ca(2+) dependent migration in DCs. Vitamin E (VitE) is an essential antioxidant to prot...

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Autores principales: Xuan, Nguyen Thi, Trang, Phi Thi Thu, Van Phong, Nguyen, Toan, Nguyen Linh, Trung, Do Minh, Bac, Nguyen Duy, Nguyen, Viet Linh, Hoang, Nguyen Huy, Van Hai, Nong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5121936/
https://www.ncbi.nlm.nih.gov/pubmed/27881156
http://dx.doi.org/10.1186/s40659-016-0105-4
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author Xuan, Nguyen Thi
Trang, Phi Thi Thu
Van Phong, Nguyen
Toan, Nguyen Linh
Trung, Do Minh
Bac, Nguyen Duy
Nguyen, Viet Linh
Hoang, Nguyen Huy
Van Hai, Nong
author_facet Xuan, Nguyen Thi
Trang, Phi Thi Thu
Van Phong, Nguyen
Toan, Nguyen Linh
Trung, Do Minh
Bac, Nguyen Duy
Nguyen, Viet Linh
Hoang, Nguyen Huy
Van Hai, Nong
author_sort Xuan, Nguyen Thi
collection PubMed
description BACKGROUND: Dendritic cells (DCs) are the most potent professional antigen-presenting cells for naive T cells to link innate and acquired immunity. Klotho, an anti-aging protein, participates in the regulation of Ca(2+) dependent migration in DCs. Vitamin E (VitE) is an essential antioxidant to protect cells from damage and elicits its inhibitory effects on NF-κB-mediated inflammatory response. However, the roles of VitE on mouse DC functions and the contribution of klotho to those effects both are unknown. The present study explored the effects of VitE on klotho expression, maturation, ROS production and migration in DCs. METHODS: The mouse bone marrow cells were isolated and cultured with GM-CSF to attain bone marrow-derived DCs (BMDCs). Cells were stimulated with LPS (100 ng/ml) in the presence or absence of VitE (500 µM). RT-PCR and immunoprecipitation methods were employed to determine klotho expression, ELISA to determine cytokine release, flow cytometry to analyze number of CD86(+)CD11c(+) cells, the intracellular expression of cytokines and reactive oxygen species (ROS) production and a transwell migration assay to trace migration. RESULTS: Klotho transcript level and this hormone secretion in DC supernatant were enhanced by VitE treatment and further increased in the presence of NF-κB inhibitor Bay 11-7082 (10 µM). Moreover, VitE treatment inhibited IL-12p70 protein expression of, ROS accumulation in and CCL21-dependent migration of LPS-triggered mature DCs, these effects were reversed following klotho silencing. CONCLUSION: The up-regulation of klotho by VitE could contribute to the inhibitory effects of VitE on NF-κB-mediated DC functional maturation. The events might contribute to immunotherapeutic effect of VitE on the pathophysiology of klotho-related disease.
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spelling pubmed-51219362016-11-30 Klotho sensitive regulation of dendritic cell functions by vitamin E Xuan, Nguyen Thi Trang, Phi Thi Thu Van Phong, Nguyen Toan, Nguyen Linh Trung, Do Minh Bac, Nguyen Duy Nguyen, Viet Linh Hoang, Nguyen Huy Van Hai, Nong Biol Res Research Article BACKGROUND: Dendritic cells (DCs) are the most potent professional antigen-presenting cells for naive T cells to link innate and acquired immunity. Klotho, an anti-aging protein, participates in the regulation of Ca(2+) dependent migration in DCs. Vitamin E (VitE) is an essential antioxidant to protect cells from damage and elicits its inhibitory effects on NF-κB-mediated inflammatory response. However, the roles of VitE on mouse DC functions and the contribution of klotho to those effects both are unknown. The present study explored the effects of VitE on klotho expression, maturation, ROS production and migration in DCs. METHODS: The mouse bone marrow cells were isolated and cultured with GM-CSF to attain bone marrow-derived DCs (BMDCs). Cells were stimulated with LPS (100 ng/ml) in the presence or absence of VitE (500 µM). RT-PCR and immunoprecipitation methods were employed to determine klotho expression, ELISA to determine cytokine release, flow cytometry to analyze number of CD86(+)CD11c(+) cells, the intracellular expression of cytokines and reactive oxygen species (ROS) production and a transwell migration assay to trace migration. RESULTS: Klotho transcript level and this hormone secretion in DC supernatant were enhanced by VitE treatment and further increased in the presence of NF-κB inhibitor Bay 11-7082 (10 µM). Moreover, VitE treatment inhibited IL-12p70 protein expression of, ROS accumulation in and CCL21-dependent migration of LPS-triggered mature DCs, these effects were reversed following klotho silencing. CONCLUSION: The up-regulation of klotho by VitE could contribute to the inhibitory effects of VitE on NF-κB-mediated DC functional maturation. The events might contribute to immunotherapeutic effect of VitE on the pathophysiology of klotho-related disease. BioMed Central 2016-11-24 /pmc/articles/PMC5121936/ /pubmed/27881156 http://dx.doi.org/10.1186/s40659-016-0105-4 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Xuan, Nguyen Thi
Trang, Phi Thi Thu
Van Phong, Nguyen
Toan, Nguyen Linh
Trung, Do Minh
Bac, Nguyen Duy
Nguyen, Viet Linh
Hoang, Nguyen Huy
Van Hai, Nong
Klotho sensitive regulation of dendritic cell functions by vitamin E
title Klotho sensitive regulation of dendritic cell functions by vitamin E
title_full Klotho sensitive regulation of dendritic cell functions by vitamin E
title_fullStr Klotho sensitive regulation of dendritic cell functions by vitamin E
title_full_unstemmed Klotho sensitive regulation of dendritic cell functions by vitamin E
title_short Klotho sensitive regulation of dendritic cell functions by vitamin E
title_sort klotho sensitive regulation of dendritic cell functions by vitamin e
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5121936/
https://www.ncbi.nlm.nih.gov/pubmed/27881156
http://dx.doi.org/10.1186/s40659-016-0105-4
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