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Expanding and exploring the diversity of phytoplasmas from lucerne (Medicago sativa)
Phytoplasmas are a group of insect-vectored bacteria responsible for disease in many plant species worldwide. Among the crop species affected is the economically valuable forage species lucerne. Here we provide comprehensive molecular evidence for infection in multiple lucerne plants by a phytoplasm...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5123570/ https://www.ncbi.nlm.nih.gov/pubmed/27886229 http://dx.doi.org/10.1038/srep37746 |
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author | Gopurenko, David Fletcher, Murray J. Liu, Jian Gurr, Geoff M. |
author_facet | Gopurenko, David Fletcher, Murray J. Liu, Jian Gurr, Geoff M. |
author_sort | Gopurenko, David |
collection | PubMed |
description | Phytoplasmas are a group of insect-vectored bacteria responsible for disease in many plant species worldwide. Among the crop species affected is the economically valuable forage species lucerne. Here we provide comprehensive molecular evidence for infection in multiple lucerne plants by a phytoplasma not previously known from this plant species. This phytoplasma had a >99% genetic similarity to an unclassified 16S rRNA subgroup previously reported as Stylosanthes little leaf from Stylosanthes spp. and was genetically and symptomatically distinct from a co-occurring but less common 16SrIIA group phytoplasma. Neighbour-joining analyses with publicly available sequence data confirmed the presence of two distinct phytoplasma lineages in the plant population. No PCR detections were made among 38 individuals of 12 co-occurring weed species. Sequence analysis revealed that all nine PCR detections from among 106 individuals of five Hemiptera insect species from the site, three of which had previously been reported as likely vectors, were false positives. This study demonstrates the importance of sequencing to complement PCR detection and avoid potentially inaccurate conclusions regarding vectors, highlights that sampling over a wide spatio-temporal scale is important for vector and alternative host studies, and extends to eight the number of phytoplasma 16 Sr groups known from lucerne. |
format | Online Article Text |
id | pubmed-5123570 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-51235702016-12-07 Expanding and exploring the diversity of phytoplasmas from lucerne (Medicago sativa) Gopurenko, David Fletcher, Murray J. Liu, Jian Gurr, Geoff M. Sci Rep Article Phytoplasmas are a group of insect-vectored bacteria responsible for disease in many plant species worldwide. Among the crop species affected is the economically valuable forage species lucerne. Here we provide comprehensive molecular evidence for infection in multiple lucerne plants by a phytoplasma not previously known from this plant species. This phytoplasma had a >99% genetic similarity to an unclassified 16S rRNA subgroup previously reported as Stylosanthes little leaf from Stylosanthes spp. and was genetically and symptomatically distinct from a co-occurring but less common 16SrIIA group phytoplasma. Neighbour-joining analyses with publicly available sequence data confirmed the presence of two distinct phytoplasma lineages in the plant population. No PCR detections were made among 38 individuals of 12 co-occurring weed species. Sequence analysis revealed that all nine PCR detections from among 106 individuals of five Hemiptera insect species from the site, three of which had previously been reported as likely vectors, were false positives. This study demonstrates the importance of sequencing to complement PCR detection and avoid potentially inaccurate conclusions regarding vectors, highlights that sampling over a wide spatio-temporal scale is important for vector and alternative host studies, and extends to eight the number of phytoplasma 16 Sr groups known from lucerne. Nature Publishing Group 2016-11-25 /pmc/articles/PMC5123570/ /pubmed/27886229 http://dx.doi.org/10.1038/srep37746 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Gopurenko, David Fletcher, Murray J. Liu, Jian Gurr, Geoff M. Expanding and exploring the diversity of phytoplasmas from lucerne (Medicago sativa) |
title | Expanding and exploring the diversity of phytoplasmas from lucerne (Medicago sativa) |
title_full | Expanding and exploring the diversity of phytoplasmas from lucerne (Medicago sativa) |
title_fullStr | Expanding and exploring the diversity of phytoplasmas from lucerne (Medicago sativa) |
title_full_unstemmed | Expanding and exploring the diversity of phytoplasmas from lucerne (Medicago sativa) |
title_short | Expanding and exploring the diversity of phytoplasmas from lucerne (Medicago sativa) |
title_sort | expanding and exploring the diversity of phytoplasmas from lucerne (medicago sativa) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5123570/ https://www.ncbi.nlm.nih.gov/pubmed/27886229 http://dx.doi.org/10.1038/srep37746 |
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