Control of bacterial nitrate assimilation by stabilization of G-quadruplex DNA

Here we present a chemical-biology study in the model soil bacterium Paracoccus denitrificans, where we show ligand-specific control of nitrate assimilation. Stabilization of a G-quadruplex in the promoter region of the nas genes, encoding the assimilatory nitrate/nitrite reductase system, is achiev...

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Detalles Bibliográficos
Autores principales: Waller, Zoë A. E., Pinchbeck, Benjamin J., Buguth, Bhovina Seewoodharry, Meadows, Timothy G., Richardson, David J., Gates, Andrew J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2016
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5123632/
https://www.ncbi.nlm.nih.gov/pubmed/27805200
http://dx.doi.org/10.1039/c6cc06057a
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author Waller, Zoë A. E.
Pinchbeck, Benjamin J.
Buguth, Bhovina Seewoodharry
Meadows, Timothy G.
Richardson, David J.
Gates, Andrew J.
author_facet Waller, Zoë A. E.
Pinchbeck, Benjamin J.
Buguth, Bhovina Seewoodharry
Meadows, Timothy G.
Richardson, David J.
Gates, Andrew J.
author_sort Waller, Zoë A. E.
collection PubMed
description Here we present a chemical-biology study in the model soil bacterium Paracoccus denitrificans, where we show ligand-specific control of nitrate assimilation. Stabilization of a G-quadruplex in the promoter region of the nas genes, encoding the assimilatory nitrate/nitrite reductase system, is achieved using known quadruplex ligands and results in attenuation of gene transcription.
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spelling pubmed-51236322016-12-05 Control of bacterial nitrate assimilation by stabilization of G-quadruplex DNA Waller, Zoë A. E. Pinchbeck, Benjamin J. Buguth, Bhovina Seewoodharry Meadows, Timothy G. Richardson, David J. Gates, Andrew J. Chem Commun (Camb) Chemistry Here we present a chemical-biology study in the model soil bacterium Paracoccus denitrificans, where we show ligand-specific control of nitrate assimilation. Stabilization of a G-quadruplex in the promoter region of the nas genes, encoding the assimilatory nitrate/nitrite reductase system, is achieved using known quadruplex ligands and results in attenuation of gene transcription. Royal Society of Chemistry 2016-11-28 2016-11-01 /pmc/articles/PMC5123632/ /pubmed/27805200 http://dx.doi.org/10.1039/c6cc06057a Text en This journal is © The Royal Society of Chemistry 2016 http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Chemistry
Waller, Zoë A. E.
Pinchbeck, Benjamin J.
Buguth, Bhovina Seewoodharry
Meadows, Timothy G.
Richardson, David J.
Gates, Andrew J.
Control of bacterial nitrate assimilation by stabilization of G-quadruplex DNA
title Control of bacterial nitrate assimilation by stabilization of G-quadruplex DNA
title_full Control of bacterial nitrate assimilation by stabilization of G-quadruplex DNA
title_fullStr Control of bacterial nitrate assimilation by stabilization of G-quadruplex DNA
title_full_unstemmed Control of bacterial nitrate assimilation by stabilization of G-quadruplex DNA
title_short Control of bacterial nitrate assimilation by stabilization of G-quadruplex DNA
title_sort control of bacterial nitrate assimilation by stabilization of g-quadruplex dna
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5123632/
https://www.ncbi.nlm.nih.gov/pubmed/27805200
http://dx.doi.org/10.1039/c6cc06057a
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