Protocol for the purification of protected carbohydrates: toward coupling automated synthesis to alternate-pump recycling high-performance liquid chromatography

Given recent advances in automated oligosaccharide synthesis, analytical techniques that can be coupled to a synthetic framework are needed to not just identify but also purify to homogeneity protected carbohydrate compounds at levels of ≥99.5% purity. Herein, an alternate-pump recycling high-perfor...

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Detalles Bibliográficos
Autores principales: Nagy, Gabe, Peng, Tianyuan, Kabotso, Daniel E. K., Novotny, Milos V., Pohl, Nicola L. B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2016
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5123635/
https://www.ncbi.nlm.nih.gov/pubmed/27775116
http://dx.doi.org/10.1039/c6cc07584c
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author Nagy, Gabe
Peng, Tianyuan
Kabotso, Daniel E. K.
Novotny, Milos V.
Pohl, Nicola L. B.
author_facet Nagy, Gabe
Peng, Tianyuan
Kabotso, Daniel E. K.
Novotny, Milos V.
Pohl, Nicola L. B.
author_sort Nagy, Gabe
collection PubMed
description Given recent advances in automated oligosaccharide synthesis, analytical techniques that can be coupled to a synthetic framework are needed to not just identify but also purify to homogeneity protected carbohydrate compounds at levels of ≥99.5% purity. Herein, an alternate-pump recycling high-performance liquid chromatography (R-HPLC) method has been developed to allow purification of protected carbohydrates at levels of ≥99.5% purity.
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spelling pubmed-51236352016-12-05 Protocol for the purification of protected carbohydrates: toward coupling automated synthesis to alternate-pump recycling high-performance liquid chromatography Nagy, Gabe Peng, Tianyuan Kabotso, Daniel E. K. Novotny, Milos V. Pohl, Nicola L. B. Chem Commun (Camb) Chemistry Given recent advances in automated oligosaccharide synthesis, analytical techniques that can be coupled to a synthetic framework are needed to not just identify but also purify to homogeneity protected carbohydrate compounds at levels of ≥99.5% purity. Herein, an alternate-pump recycling high-performance liquid chromatography (R-HPLC) method has been developed to allow purification of protected carbohydrates at levels of ≥99.5% purity. Royal Society of Chemistry 2016-11-21 2016-10-24 /pmc/articles/PMC5123635/ /pubmed/27775116 http://dx.doi.org/10.1039/c6cc07584c Text en This journal is © The Royal Society of Chemistry 2016 http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Chemistry
Nagy, Gabe
Peng, Tianyuan
Kabotso, Daniel E. K.
Novotny, Milos V.
Pohl, Nicola L. B.
Protocol for the purification of protected carbohydrates: toward coupling automated synthesis to alternate-pump recycling high-performance liquid chromatography
title Protocol for the purification of protected carbohydrates: toward coupling automated synthesis to alternate-pump recycling high-performance liquid chromatography
title_full Protocol for the purification of protected carbohydrates: toward coupling automated synthesis to alternate-pump recycling high-performance liquid chromatography
title_fullStr Protocol for the purification of protected carbohydrates: toward coupling automated synthesis to alternate-pump recycling high-performance liquid chromatography
title_full_unstemmed Protocol for the purification of protected carbohydrates: toward coupling automated synthesis to alternate-pump recycling high-performance liquid chromatography
title_short Protocol for the purification of protected carbohydrates: toward coupling automated synthesis to alternate-pump recycling high-performance liquid chromatography
title_sort protocol for the purification of protected carbohydrates: toward coupling automated synthesis to alternate-pump recycling high-performance liquid chromatography
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5123635/
https://www.ncbi.nlm.nih.gov/pubmed/27775116
http://dx.doi.org/10.1039/c6cc07584c
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