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MRI-based assessment of liver perfusion and hepatocyte injury in the murine model of acute hepatitis
OBJECTIVE: To assess alterations in perfusion and liver function in the concanavalin A (ConA)-induced mouse model of acute liver failure (ALF) using two magnetic resonance imaging (MRI)-based methods: dynamic contrast-enhanced MRI (DCE-MRI) with Gd-EOB-DTPA contrast agent and arterial spin labelling...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5124046/ https://www.ncbi.nlm.nih.gov/pubmed/27160299 http://dx.doi.org/10.1007/s10334-016-0563-2 |
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author | Byk, Katarzyna Jasinski, Krzysztof Bartel, Zaneta Jasztal, Agnieszka Sitek, Barbara Tomanek, Boguslaw Chlopicki, Stefan Skorka, Tomasz |
author_facet | Byk, Katarzyna Jasinski, Krzysztof Bartel, Zaneta Jasztal, Agnieszka Sitek, Barbara Tomanek, Boguslaw Chlopicki, Stefan Skorka, Tomasz |
author_sort | Byk, Katarzyna |
collection | PubMed |
description | OBJECTIVE: To assess alterations in perfusion and liver function in the concanavalin A (ConA)-induced mouse model of acute liver failure (ALF) using two magnetic resonance imaging (MRI)-based methods: dynamic contrast-enhanced MRI (DCE-MRI) with Gd-EOB-DTPA contrast agent and arterial spin labelling (ASL). MATERIALS AND METHODS: BALB/c mice were studied using a 9.4 T MRI system. The IntraGateFLASH(TM) and FAIR-EPI pulse sequences were used for optimum mouse abdomen imaging. RESULTS: The average perfusion values for the liver of the control and ConA group were equal to 245 ± 20 and 200 ± 32 ml/min/100 g (p = 0.008, respectively). DCE-MRI showed that the time to the peak of the image enhancement was 6.14 ± 1.07 min and 9.72 ± 1.69 min in the control and ConA group (p < 0.001, respectively), while the rate of the contrast wash-out in the control and ConA group was 0.037 ± 0.008 and 0.021 ± 0.008 min(−1) (p = 0.004, respectively). These results were consistent with hepatocyte injury in the ConA-treated mice as confirmed by histopathological staining. CONCLUSIONS: Both the ASL and DCE-MRI techniques represent a reliable methodology to assess alterations in liver perfusion and hepatocyte integrity in murine hepatitis. |
format | Online Article Text |
id | pubmed-5124046 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-51240462016-12-09 MRI-based assessment of liver perfusion and hepatocyte injury in the murine model of acute hepatitis Byk, Katarzyna Jasinski, Krzysztof Bartel, Zaneta Jasztal, Agnieszka Sitek, Barbara Tomanek, Boguslaw Chlopicki, Stefan Skorka, Tomasz MAGMA Research Article OBJECTIVE: To assess alterations in perfusion and liver function in the concanavalin A (ConA)-induced mouse model of acute liver failure (ALF) using two magnetic resonance imaging (MRI)-based methods: dynamic contrast-enhanced MRI (DCE-MRI) with Gd-EOB-DTPA contrast agent and arterial spin labelling (ASL). MATERIALS AND METHODS: BALB/c mice were studied using a 9.4 T MRI system. The IntraGateFLASH(TM) and FAIR-EPI pulse sequences were used for optimum mouse abdomen imaging. RESULTS: The average perfusion values for the liver of the control and ConA group were equal to 245 ± 20 and 200 ± 32 ml/min/100 g (p = 0.008, respectively). DCE-MRI showed that the time to the peak of the image enhancement was 6.14 ± 1.07 min and 9.72 ± 1.69 min in the control and ConA group (p < 0.001, respectively), while the rate of the contrast wash-out in the control and ConA group was 0.037 ± 0.008 and 0.021 ± 0.008 min(−1) (p = 0.004, respectively). These results were consistent with hepatocyte injury in the ConA-treated mice as confirmed by histopathological staining. CONCLUSIONS: Both the ASL and DCE-MRI techniques represent a reliable methodology to assess alterations in liver perfusion and hepatocyte integrity in murine hepatitis. Springer Berlin Heidelberg 2016-05-09 2016 /pmc/articles/PMC5124046/ /pubmed/27160299 http://dx.doi.org/10.1007/s10334-016-0563-2 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Research Article Byk, Katarzyna Jasinski, Krzysztof Bartel, Zaneta Jasztal, Agnieszka Sitek, Barbara Tomanek, Boguslaw Chlopicki, Stefan Skorka, Tomasz MRI-based assessment of liver perfusion and hepatocyte injury in the murine model of acute hepatitis |
title | MRI-based assessment of liver perfusion and hepatocyte injury in the murine model of acute hepatitis |
title_full | MRI-based assessment of liver perfusion and hepatocyte injury in the murine model of acute hepatitis |
title_fullStr | MRI-based assessment of liver perfusion and hepatocyte injury in the murine model of acute hepatitis |
title_full_unstemmed | MRI-based assessment of liver perfusion and hepatocyte injury in the murine model of acute hepatitis |
title_short | MRI-based assessment of liver perfusion and hepatocyte injury in the murine model of acute hepatitis |
title_sort | mri-based assessment of liver perfusion and hepatocyte injury in the murine model of acute hepatitis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5124046/ https://www.ncbi.nlm.nih.gov/pubmed/27160299 http://dx.doi.org/10.1007/s10334-016-0563-2 |
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